Invitrogen

CD3e Monoclonal Antibody (eBio500A2 (500A2)), Alexa Fluor™ 700, eBioscience™

FIGURE: 1 / 4

CD3e Antibody (56-0033-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Anti-Mouse CD3e Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

CD3e Antibody in Immunohistochemistry (IHC)

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Product Details

56-0033-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBio500A2 (500A2)

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837094

Product Specific Information

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity.

PLoS neglected tropical diseases

Bhattacharya P,Ismail N,Saxena A,Gannavaram S,Dey R,Oljuskin T,Akue A,Takeda K,Yu J,Karmakar S,Dagur PK,McCoy JP,Nakhasi HL

56-0033-82 was used in Flow Cytometry to show that LdCen-/- containing neutrophils phagocytized by DC markedly influence the phenotype and antigen presenting capacity of DCs early on and thus play an immune-regulatory role in shaping vaccine induced host protective response.

Tue Feb 01 00:00:00 EST 2022

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using CD3e monoclonal antibody (Product # 56-0033-82) in Immunohistochemistry

Tue Nov 23 00:00:00 EST 2021

Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2.

iScience

Park JG,Oladunni FS,Rohaim MA,Whittingham-Dowd J,Tollitt J,Hodges MDJ,Fathallah N,Assas MB,Alhazmi W,Almilaibary A,Iqbal M,Chang P,Escalona R,Shivanna V,Torrelles JB,Worthington JJ,Jackson-Jones LH,Martinez-Sobrido L,Munir M

56-0033-82 was used in Flow Cytometry to evaluate a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2.

Fri Sep 24 00:00:00 EDT 2021

Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist.

eLife

Glassman CR,Su L,Majri-Morrison SS,Winkelmann H,Mo F,Li P,Pérez-Cruz M,Ho PP,Koliesnik I,Nagy N,Hnizdilova T,Picton LK,Kovar M,Bollyky P,Steinman L,Meyer E,Piehler J,Leonard WJ,Garcia KC

56-0033 was used in Flow cytometry/Cell sorting to show that cytokine partial agonists can be used to calibrate intrinsic differences in response thresholds across responding cell types to narrow pleiotropic actions, which may be generalizable to other cytokine and growth factor systems.

Tue May 18 00:00:00 EDT 2021

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD3e monoclonal antibody (Product # 56-0033-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferrets.

Nature communications

Kwak JE,Kim YI,Park SJ,Yu MA,Kwon HI,Eo S,Kim TS,Seok J,Choi WS,Jeong JH,Lee H,Cho Y,Kwon JA,Jeong M,Maslow JN,Kim YE,Jeon H,Kim KK,Shin EC,Song MS,Jung JU,Choi YK,Park SH

56-0033 was used in Flow cytometry/Cell sorting to investigate the development of the SFTSV DNA vaccine in ferrets.

Fri Aug 23 00:00:00 EDT 2019

Herpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell Immunity.

Immune network

Kim AR,Park J,Kim JH,Kwak JE,Cho Y,Lee H,Jeong M,Park SH,Shin EC

56-0033 was used in Flow cytometry/Cell sorting to evaluate the T cell immunogenicity of Herpes Zoster DNA vaccines.

Mon Oct 01 00:00:00 EDT 2018

γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

Nature communications

Rezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HL

56-0033 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

Wed Aug 08 00:00:00 EDT 2018

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

56-0033-82 was used in Flow Cytometry to compare the innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Sat Jan 09 00:00:00 EST 2021

Cytokine and hormonal regulation of bone marrow immune cell Wnt10b expression.

PloS one

Collins FL,Rios-Arce ND,McCabe LR,Parameswaran N

56-0033 was used in Flow cytometry/Cell sorting to determine the potential regulation of Wnt10b by estrogen and TNFα, showing regulation of expression by cytokines and hormones.

Mon Oct 09 00:00:00 EDT 2017

Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential.

PloS one

Qualai J,Li LX,Cantero J,Tarrats A,Fernández MA,Sumoy L,Rodolosse A,McSorley SJ,Genescà M

56-0033 was used in Flow cytometry/Cell sorting to find markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T cells.

Thu Mar 16 00:00:00 EDT 2017

Selective Targeting of a Disease-Related Conformational Isoform of Macrophage Migration Inhibitory Factor Ameliorates Inflammatory Conditions.

Journal of immunology (Baltimore, Md. : 1950)

Thiele M,Kerschbaumer RJ,Tam FW,Völkel D,Douillard P,Schinagl A,Kühnel H,Smith J,McDaid JP,Bhangal G,Yu MC,Pusey CD,Cook HT,Kovarik J,Magelky E,Bhan A,Rieger M,Mudde GC,Ehrlich H,Jilma B,Tilg H,Moschen A,Terhorst C,Scheiflinger F

56-0033 was used in Flow cytometry/Cell sorting to demonstrate that oxMIF represents the disease-related isoform of MIF and is therefore a new diagnostic marker for inflammation.

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3e Monoclonal Antibody (eBio500A2 (500A2)), Alexa Fluor™ 700, eBioscience™

CD3e Antibody (56-0033-82) in Flow

Product Details

56-0033-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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