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CD4 Antibody (MA5-29110) in WB
Western Blot using CD4 Recombinant Rabbit Monoclonal Antibody (104) (Product # MA5-29110) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 62 kDa.
Product Details
MA5-29110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
104
Immunogen
Recombinant Human CD4 protein (Met1-Trp390)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785037
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CD4/Leu-3.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Gene Aliases: CD4; CD4mut
UniProt ID: (Human) P01730
Entrez Gene ID: (Human) 920
virus receptor activity
glycoprotein binding
receptor activity
transmembrane signaling receptor activity
extracellular matrix structural constituent
protein binding
zinc ion binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein binding
protein homodimerization activity
protein tyrosine kinase binding
cytokine production
adaptive immune response
induction by virus of host cell-cell fusion
immune response
signal transduction
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of calcium ion transport into cytosol
fusion of virus membrane with host plasma membrane
T cell differentiation
entry into host cell
T cell costimulation
response to estradiol
maintenance of protein location in cell
response to vitamin D
helper T cell enhancement of adaptive immune response
positive regulation of T cell proliferation
T cell selection
positive regulation of interleukin-2 biosynthetic process
positive regulation of protein kinase activity
regulation of defense response to virus by virus
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
regulation of T cell activation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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