Invitrogen

CD4 Monoclonal Antibody (RM4-5), PE

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CD4 Antibody (MCD0404) in Flow

Cell death discovery 2021 - Fig. 3 Wip1 -/- MSCs exhibited lower immunosuppression response in T1DM mice. A Frequency of CD4 + IFN-gamma + Th1 cells was analyzed by flow cytometry in MSCs-treated T1DM mice. B The quantitative results for CD4 + IFN-gamma + Th1 cells in splenic lymphocytes. C Serum IFN-gamma, IL-17a, and IL-4 were detected by ELISA. ( n = 7, three independent experiments) ns, not significant, * P < 0.05, ** P < 0.01.

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Product Details

MCD0404

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Immunogen

Mouse CD4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10392550

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2.

Cell death discovery

Zhou N,Liu W,Zhang W,Liu Y,Li X,Wang Y,Zheng R,Zhang Y

Published figure using CD4 monoclonal antibody (Product # MCD0404) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Histone deacetylase 1 and 2 are essential for normal T-cell development and genomic stability in mice.

Blood

Dovey OM,Foster CT,Conte N,Edwards SA,Edwards JM,Singh R,Vassiliou G,Bradley A,Cowley SM

Published figure using CD4 monoclonal antibody (Product # MCD0404) in Flow Cytometry

Thu Feb 21 00:00:00 EST 2013

CD4+ Foxp3+ regulatory T cells mediate Toxoplasma gondii-induced T-cell suppression through an IL-2-related mechanism but independently of IL-10.

European journal of immunology

Tenorio EP,Fernández J,Castellanos C,Olguín JE,Saavedra R

MCD0404 was used in Flow cytometry/Cell sorting to study the role of Treg cells in Toxoplasma gondii induced suppression.

Thu Dec 01 00:00:00 EST 2011

The ΔfbpA attenuated candidate vaccine from Mycobacterium tuberculosis, H37Rv primes for a stronger T-bet dependent Th1 immunity in mice.

Tuberculosis (Edinburgh, Scotland)

Roche CM,Smith A,Lindsey DR,Meher A,Schluns K,Arora A,Armitige LY,Jagannath C

Published figure using CD4 monoclonal antibody (Product # MCD0404) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Th17 cells are involved in the local control of tumor progression in primary intraocular lymphoma.

PloS one

Galand C,Donnou S,Crozet L,Brunet S,Touitou V,Ouakrim H,Fridman WH,Sautès-Fridman C,Fisson S

MCD0404 was used in flow cytometry to examine Th17 cells in the microenvironment of primary intraocular B-cell lymphoma

Fri Feb 03 00:00:00 EST 2012

Dysregulated macrophage-inflammatory protein-2 expression drives illness in bacterial superinfection of influenza.

Journal of immunology (Baltimore, Md. : 1950)

Zavitz CC,Bauer CM,Gaschler GJ,Fraser KM,Strieter RM,Hogaboam CM,Stampfli MR

MCD0404 was used in flow cytometry to develop and characterize a novel model of heterologous pulmonary infection using Bordetella parapertussis and influenza virus

Mon Feb 15 00:00:00 EST 2010

Early infection termination affects number of CD8+ memory T cells and protective capacities in listeria monocytogenes-infected mice upon rechallenge.

Journal of immunology (Baltimore, Md. : 1950)

Tseng KE,Chung CY,H'ng WS,Wang SL

MCD0404 was used in flow cytometry to assess the effects of early termination of infection on primary T cell expansion, subsequent memory cell development, and protective immunity

Wed Apr 15 00:00:00 EDT 2009

Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6.

Journal of immunology (Baltimore, Md. : 1950)

King SB,Knorn AM,Ohnmacht C,Voehringer D

MCD0404 was used in flow cytometry to investigate the effect of Stat6 in T cell expansion, survival, and recruitment to the lung

Tue Jan 15 00:00:00 EST 2008

Dectin-1 is required for beta-glucan recognition and control of fungal infection.

Nature immunology

Taylor PR,Tsoni SV,Willment JA,Dennehy KM,Rosas M,Findon H,Haynes K,Steele C,Botto M,Gordon S,Brown GD

MCD0404 was used in flow cytometry to report that dectin-1 deficiency renders mice susceptible to infection with Candida albicans

Mon Jan 01 00:00:00 EST 2007

Complement C5a anaphylatoxin is an innate determinant of dendritic cell-induced Th1 immunity to Mycobacterium bovis BCG infection in mice.

Journal of leukocyte biology

Moulton RA,Mashruwala MA,Smith AK,Lindsey DR,Wetsel RA,Haviland DL,Hunter RL,Jagannath C

MCD0404 was used in flow cytometry to assess the effects of C5a on IL-12p70 secretion by murine dendritic cells and Th1 immunity

Mon Oct 01 00:00:00 EDT 2007

Characterization of early gamma interferon (IFN-gamma) expression during murine listeriosis: identification of NK1.1+ CD11c+ cells as the primary IFN-gamma-expressing cells.

Infection and immunity

Chang SR,Wang KJ,Lu YF,Yang LJ,Chen WJ,Lin YH,Chang HH,Wang SL

MCD0404 was used in flow cytometry to identify the cells that are producing gamma interferon during infection with Listeria monocytogenes

Thu Mar 01 00:00:00 EST 2007

Renal ischemia-reperfusion injury and adenosine 2A receptor-mediated tissue protection: the role of CD4+ T cells and IFN-gamma.

Journal of immunology (Baltimore, Md. : 1950)

Day YJ,Huang L,Ye H,Li L,Linden J,Okusa MD

MCD0404 was used in flow cytometry to characterize the role of CD4+ T cells and IFN-gamma and adenosine 2A receptor-mediated tissue protection and renal ischemia-reperfusion injury

Wed Mar 01 00:00:00 EST 2006

Gene therapy of experimental autoimmune thyroiditis by in vivo administration of plasmid DNA coding for Fas ligand.

Journal of immunology (Baltimore, Md. : 1950)

Batteux F,Tourneur L,Trebeden H,Charreire J,Chiocchia G

MCD0404 was used in flow cytometry to study FasL expression on thyrocytes.

Fri Jan 01 00:00:00 EST 1999

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CD4 Monoclonal Antibody (RM4-5), PE

CD4 Antibody (MCD0404) in Flow

Product Details

MCD0404

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Performance Guarantee

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