Invitrogen

CD40 Monoclonal Antibody (3/23)

Product Details

MA1-81395

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50

View 10 publications 10 publications

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3/23

Immunogen

Extracellular domain of Murine CD40 and the Fc portion of Human IgG1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928280

Product Specific Information

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model.

The Prostate

Sulek JE,Robinson SP,Petrossian AA,Zhou S,Goliadze E,Manjili MH,Toor A,Guruli G

MA181395 was used in flow cytometry to study cancer-testis antigen expression in a mouse model of prostate cancer

Wed Mar 01 00:00:00 EST 2017

Purified eicosapentaenoic acid induces prolonged survival of cardiac allografts and generates regulatory T cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Iwami D,Zhang Q,Aramaki O,Nonomura K,Shirasugi N,Niimi M

MA1-81395 was used in flow cytometry to test if eicosapentaenoic acid treatment alters the survival of fully mismatched murine cardiac allografts

Mon Jun 01 00:00:00 EDT 2009

Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.

The Journal of experimental medicine

Chen D,Carpenter A,Abrahams J,Chambers RC,Lechler RI,McVey JH,Dorling A

MA1-81395 was used in immunocytochemistry to study the role of PAR activation in vivo to initiate MCP-dependent leukocyte recruitment

Mon Aug 04 00:00:00 EDT 2008

Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors.

Blood

Zhang J,Raper A,Sugita N,Hingorani R,Salio M,Palmowski MJ,Cerundolo V,Crocker PR

MA1-81395 was used in flow cytometry to clone and characterize Siglec-H

Mon May 01 00:00:00 EDT 2006

A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D.

European journal of immunology

Savithri B,Khar A

MA1-81395 was used in flow cytometry to identify the ligand for the rat ortholog of human and mouse NKG2D

Sun Jan 01 00:00:00 EST 2006

Activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles are enhanced by CpG ODN or sorbitol.

Antiviral therapy

Freyschmidt EJ,Alonso A,Hartmann G,Gissmann L

MA1-81395 was used in flow cytometry to study the interaction of human papillomavirus 16 L1/E7 chimeric virus-like particles with mouse bone marrow-derived dendritic cells activated with different immune adjuvants

Sun Aug 01 00:00:00 EDT 2004

Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.

Clinical and experimental immunology

Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S

MA1-81395 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.

Mon Dec 01 00:00:00 EST 2003

Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Kumari AL,Patel M,Khar A

MA1-81395 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells

Mon Oct 01 00:00:00 EDT 2001

Uptake and presentation of antigen to T cells by primary colonic epithelial cells in normal and diseased states.

Gastroenterology

Telega GW,Baumgart DC,Carding SR

MA1-81395 was used in flow cytometry to determine the immunoregulatory properties of primary colonic epithelial cells.

Fri Dec 01 00:00:00 EST 2000

Efficient suicide gene therapy of transduced and distant untransduced ovary tumors is correlated with significant increase of intratumoral T and NK cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Nagy HJ,Panis Y,Fabre M,Engelmann C,Soubrane O,Houssin D,Klatzmann D

MA1-81395 was used in immunohistochemistry to study the mechanism for the suicide gene therapy of ovary tumors

Sun Oct 01 00:00:00 EDT 2000

Target-cell-induced anergy in natural killer cells: suppression of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Khar A

MA1-81395 was used in flow cytometry to investigate the cytotoxic functions of natural killer cells

Mon May 01 00:00:00 EDT 2000

Dendritic cells in the induction of protective and nonprotective anticryptococcal cell-mediated immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Bauman SK,Nichols KL,Murphy JW

MA1-81395 was used in flow cytometry to examine the contribution of different DC subsets during infection with Cryptococcus neoformans.

Sat Jul 01 00:00:00 EDT 2000

Interleukin-2-induced nitric oxide synthase and nuclear factor-kappaB activity in activated natural killer cells and the production of interferon-gamma.

Scandinavian journal of immunology

Jyothi MD,Khar A

MA1-81395 was used in flow cytometry to investigate the role of interleukin 2 in the regulation of natural killer cell function

Tue Aug 01 00:00:00 EDT 2000

Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.

Cancer research

Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G

MA1-81395 was used in immunohistochemistry to evaluate the interleukin 4 gene therapy of rat malignant gliomas

Mon Feb 01 00:00:00 EST 1999

Administration of anti-IL-12 antibody in vivo inhibits rejection of a rat histiocytoma and suppresses cytokine response in a tumour-bearing host.

Immunology

Rao KL,Varalakshmi C,Ali AM,Khar A

MA1-81395 was used in flow cytometry to investigate the effect of interleukin 12 inhibition on tumor rejection

Sat Nov 01 00:00:00 EST 1997

Phospholipase A2-mediated inflammation induces regression of malignant gliomas.

Cancer letters

Goddard DH,Bomalaski JS,Lipper S,Shorr RG,Clark MA

MA1-81395 was used in immunohistochemistry to investigate the effect of phospholipase A2 on glioma inhibition in vivo

Fri Apr 19 00:00:00 EDT 1996

Linomide inhibits angiogenesis, growth, metastasis, and macrophage infiltration within rat prostatic cancers.

Cancer research

Vukanovic J,Isaacs JT

MA1-81395 was used in immunohistochemistry to study the role of mitochondrial pathway in neutrophil survival modulated by IgE in asthma

Sat Apr 01 00:00:00 EST 1995

Natural killer cell activation-associated induction of target cell DNA fragmentation in a spontaneously regressing rat histiocytoma.

Immunology

Bright JJ,Kausalya S,Khar A

MA1-81395 was used in flow cytometry to investigate the effect of natural killer cells on apoptosis in a regressing histiocytoma

Tue Aug 01 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

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UniProt ID: (Mouse) Q99NE2

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Entrez Gene ID: (Mouse) 21939

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Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD40 Monoclonal Antibody (3/23)

Product Details

MA1-81395

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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