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CD42b Antibody (MA5-51402) in IHC (P)
Immunohistochemistry analysis of CD42b in paraffin-embedded human spleen. Samples were incubated with CD42b Recombinant monoclonal antibody (Product # MA5-51402) using a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4U5A9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 15-531 of human CD42b (NP_000164.5).
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093011
Product Specific Information
Immunogen sequence: HPHPICEVSK VASHLEVNCD KRNLTALPPD LPKDTTILHL SENLLYTFSL ATLMPYTRLT QLNLDRCELT KLQVDGTLPV LGTLDLSHNQ LQSLPLLGQT LPALTVLDVS FNRLTSLPLG ALRGLGELQE LYLKGNELKT LPPGLLTPTP KLEKLSLANN NLTELPAGLL NGLENLDTLL LQENSLYTIP KGFFGSHLLP FAFLHGNPWL CNCEILYFRR WLQDNAENVY VWKQGVDVKA MTSNVASVQC DNSDKFPVYK YPGKGCPTLG DEGDTDLYDY YPEEDTEGDK VRATRTVVKF PTKAHTTPWG LFYSWSTASL DSQMPSSLHP TQESTKEQTT FPPRWTPNFT LHMESITFSK TPKSTTEPTP SPTTSEPVPE PAPNMTTLEP TPSPTTPEPT SEPAPSPTTP EPTSEPAPSP TTPEPTSEPA PSPTTPEPTP IPTIATSPTI LVSATSLITP KSTFLTTTKP VSLLESTKKT IPELDQPPKL RGVLQGHLES SRNDPFLHPD FCCLLPL
Target Information
CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen CD42b-alpha; CD42b; glycoprotein Ib (platelet), alpha polypeptide; GP-Ib alpha; GPIB; GPIb alpha; GPIX; MGC34595; mutant platelet membrane glycoprotein Ib-alpha; Platelet glycoprotein Ib alpha chain; Platelet GPIX; platelet membrane glycoprotein 1b-alpha subunit; platelet membrane glycoprotein Ib-alpha
Gene Aliases: BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; GP1BA; GPIbA; GPIbalpha; VWDP
UniProt ID: (Human) P07359
Entrez Gene ID: (Human) 2811
cell morphogenesis
negative regulation of protein kinase activity
cell adhesion
cell surface receptor signaling pathway
blood coagulation
blood coagulation, intrinsic pathway
cytokine-mediated signaling pathway
platelet activation
regulation of blood coagulation
fibrinolysis
negative regulation of JAK-STAT cascade
thrombin receptor signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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