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Invitrogen
CD42b Monoclonal Antibody (HIP1), eBioscience™
Product Details
14-0429-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HIP1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467238
Product Specific Information
Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.
Applications Reported: The HIP1 antibody has been reported for use in flow cytometric analysis, in vitro blocking of human CD42b, immunoblotting (WB), and immunohistochemical staining. (Please use Functional Grade purified HIP1, Product # 16-0429, in functional assays).
Applications Tested: The HIP1 antibody has been tested by flow cytometric analysis of human platelets and can be used less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibodies be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antigen CD42b-alpha; CD42b; glycoprotein Ib (platelet), alpha polypeptide; GP-Ib alpha; GPIB; GPIb alpha; GPIX; MGC34595; mutant platelet membrane glycoprotein Ib-alpha; Platelet glycoprotein Ib alpha chain; Platelet GPIX; platelet membrane glycoprotein 1b-alpha subunit; platelet membrane glycoprotein Ib-alpha
Gene Aliases: BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; GP1BA; GPIbA; GPIbalpha; VWDP
UniProt ID: (Human) P07359
Entrez Gene ID: (Human) 2811
cell morphogenesis
negative regulation of protein kinase activity
cell adhesion
cell surface receptor signaling pathway
blood coagulation
blood coagulation, intrinsic pathway
cytokine-mediated signaling pathway
platelet activation
regulation of blood coagulation
fibrinolysis
negative regulation of JAK-STAT cascade
thrombin receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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