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Invitrogen
CD42b Monoclonal Antibody (HIP1), NovaFluor™ Blue 510, eBioscience™
FIGURE: 1 / 3
CD42b Antibody (H051T03B01-A) in Flow
Human platelets were unstained (left) or stained with CD42b Monoclonal Antibody, NovaFluor Blue 510 (right). All cells were co-stained with CD41a Monoclonal Antibody, eFluor 450 (Product # 14-0419-82). Total viable cells were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
H051T03B01-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HIP1
Conjugate
Excitation/Emission Max
493/513 nm
View spectra
Form
Liquid
Concentration
0.2 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3098612 (AB_2925997)
Product Specific Information
Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This HIP1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue) Laser
Target Information
CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen CD42b-alpha; CD42b; glycoprotein Ib (platelet), alpha polypeptide; GP-Ib alpha; GPIB; GPIb alpha; GPIX; MGC34595; mutant platelet membrane glycoprotein Ib-alpha; Platelet glycoprotein Ib alpha chain; Platelet GPIX; platelet membrane glycoprotein 1b-alpha subunit; platelet membrane glycoprotein Ib-alpha
Gene Aliases: BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; GP1BA; GPIbA; GPIbalpha; VWDP
UniProt ID: (Human) P07359
Entrez Gene ID: (Human) 2811
cell morphogenesis
negative regulation of protein kinase activity
cell adhesion
cell surface receptor signaling pathway
blood coagulation
blood coagulation, intrinsic pathway
cytokine-mediated signaling pathway
platelet activation
regulation of blood coagulation
fibrinolysis
negative regulation of JAK-STAT cascade
thrombin receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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