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Invitrogen
CD42d Monoclonal Antibody (1C2), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 1
CD42d Antibody (53-0421-82) in Flow
C57BL/6 mouse platelets were stained with CD41a Monoclonal Antibody, eFluor 450 (Product # 48-0411-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, Alexa Fluor 488 (Product # 53-4888-80) (left) or 0.25 µg of CD42d Monoclonal Antibody, Alexa Fluor 488 (right). Total events were used for analysis.
Product Details
53-0421-82
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1C2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2811853
Product Specific Information
Description: The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV). CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin. This functional complex plays a role in platelet adhesion. Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes.
Applications Reported: This 1C2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets. The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD42d; Glycoprotein 5; glycoprotein 5, platelet; GPV; Platelet glycoprotein 5; Platelet glycoprotein V; Platelete glycoprotein 5; Platelets GP V
Gene Aliases: Gp5; GPV; PLGPV
UniProt ID: (Rat) O08770
Entrez Gene ID: (Rat) 25259, (Mouse) 14729
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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