Invitrogen

CD44 Monoclonal Antibody (1M7.8.1)

1 Published Figure

9 References

View all (259) CD44 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

CD44 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CD44 Antibody (MA4405) in ICC/IF

Immunofluorescent analysis of CD44 (green) showing staining in the membrane and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD44 monoclonal antibody (Product # MA4405) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLig... View More

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Product Details

MA4405

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

Inhibition Assays (IA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1M7.8.1

Immunogen

Mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223593

Product Specific Information

MA4405 targets CD44 in FACS, IA, IF, IP, and WB applications and shows reactivity with mouse and Human samples.

The MA4405 immunogen is mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).

MA4405 detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product is a Low Endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations.

World journal of stem cells

Freude KK,Saruhanian S,McCauley A,Paterson C,Odette M,Oostenink A,Hyttel P,Gillies M,Haukedal H,Kolko M

MA4405 was used in Immunocytochemistry to describe, evaluate, and refine methods with which to generate Müller glia and retinal ganglion cells progenitors, isolated them via magnetic-activated cell sorting, and assessed their lineage stability after prolonged 2D culture.

Mon Oct 26 00:00:00 EDT 2020

Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations.

World journal of stem cells

Freude KK,Saruhanian S,McCauley A,Paterson C,Odette M,Oostenink A,Hyttel P,Gillies M,Haukedal H,Kolko M

Mon Oct 26 00:00:00 EDT 2020

Hyaluronic acid, CD44 and RHAMM regulate myoblast behavior during embryogenesis.

Matrix biology : journal of the International Society for Matrix Biology

Leng Y,Abdullah A,Wendt MK,Calve S

MA4405 was used in Immunohistochemistry (Frozen) to investigate the role of hyaluronic acid (HA), the relative contribution of the two types of HA receptors, RHAMM and CD44, and the intracellular signaling pathways involved in HA-mediated effects, in myogenesis.

Wed May 01 00:00:00 EDT 2019

Exploiting Arginine Auxotrophy with Pegylated Arginine Deiminase (ADI-PEG20) to Sensitize Pancreatic Cancer to Radiotherapy via Metabolic Dysregulation.

Molecular cancer therapeutics

Singh PK,Deorukhkar AA,Venkatesulu BP,Li X,Tailor R,Bomalaski JS,Krishnan S

MA4405 was used in Immunocytochemistry-immunoflourescence to explore the therapeutic potential of depleting exogenous arginine via pegylated arginine deiminase treatment as an adjunct to radiotherapy.

Sun Dec 01 00:00:00 EST 2019

DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.

The Journal of experimental medicine

Randall KL,Chan SS,Ma CS,Fung I,Mei Y,Yabas M,Tan A,Arkwright PD,Al Suwairi W,Lugo Reyes SO,Yamazaki-Nakashimada MA,Garcia-Cruz Mde L,Smart JM,Picard C,Okada S,Jouanguy E,Casanova JL,Lambe T,Cornall RJ,Russell S,Oliaro J,Tangye SG,Bertram EM,Goodnow CC

MA4405 was used in flow cytometry to determine the role for DOCK8 in peripheral CD8 T cell survival and function

Mon Oct 24 00:00:00 EDT 2011

Lymphoid environment limits superantigen and antigen-induced T cell proliferation at high precursor frequency.

European journal of immunology

Attinger A,MacDonald HR,Acha-Orbea H

MA4405 was used in flow cytometry to report that the lymphoid environment limits T cell proliferation in response to high superantigen levels.

Thu Mar 01 00:00:00 EST 2001

Intervention of CD4+ cell subset shifts and autoimmunity in the BXSB mouse by murine CTLA4Ig.

Journal of immunology (Baltimore, Md. : 1950)

Chu EB,Hobbs MV,Wilson CB,Romball CG,Linsley PS,Weigle WO

MA4405 was used in flow cytometry to investigate the effect of CTLA4lg treatment on autoimmune disease in the BXSB mouse

Thu Feb 01 00:00:00 EST 1996

Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Pavlovitch JH,Hulier E,Rizk-Rabin M,Marussig M,Mazier D,Joffret ML,Hoos S,Papiernik M

MA4405 was used in flow cytometry to investigate the role of CD8 T cells in immune responses against murine leukemia viruses

Sat Jun 15 00:00:00 EDT 1996

Distinct phenotypes of antigen-selected CD8 T cells emerge at different stages of an in vivo immune response.

Journal of immunology (Baltimore, Md. : 1950)

Walker PR,Ohteki T,Lopez JA,MacDonald HR,Maryanski JL

MA4405 was used in flow cytometry to investigate the T cell differentiation and maturation after immunization

Sun Oct 01 00:00:00 EDT 1995

Genetic characterization of a polymorphic murine cell-surface glycoprotein.

Immunogenetics

Lesley J,Trowbridge IS

MA4405 was used in immunocytochemistry to characterize a murine cell-surface glycoprotein Pgp-1

Mon Mar 01 00:00:00 EST 1982

Bioinformatics

Protein Aliases: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

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Gene Aliases: AU023126; AW121933; AW146109; CD44; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1

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UniProt ID: (Human) Q16064, (Mouse) P15379

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Entrez Gene ID: (Human) 960, (Mouse) 12505

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Function(s)

cytokine receptor activity protein binding collagen binding hyaluronic acid binding hyalurononglucosaminidase activity epidermal growth factor receptor binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

Process(es)

cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of cell growth branching involved in ureteric bud morphogenesis wound healing involved in inflammatory response positive regulation of adaptive immune response negative regulation of mature B cell apoptotic process cell adhesion positive regulation of gene expression Wnt signaling pathway cell migration neuron projection development positive regulation of neutrophil apoptotic process macrophage fusion branching involved in prostate gland morphogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD44 Monoclonal Antibody (1M7.8.1)

CD44 Antibody (MA4405) in ICC/IF

Product Details

MA4405

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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