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Leinco Technologies
CD44 Monoclonal Antibody (IM7), Functional Grade, In Vivo GOLD™
Product Details
C382-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
IM7
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient
Product Specific Information
Specificity: Clone IM7 recognizes an epitope common to alloantigens and all isoforms of CD44 that is located between amino acids 145 and 186.
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Immunoprecipitation, Immunohistochemistry-Fresh Frozen, Immunohistochemistry-Formalin-Fixed, Paraffin-Embedded, Immunocytochemistry, Flow Cytometry, ELISA Detection, CyTOF®, CODEX®.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44
Gene Aliases: AU023126; AW121933; AW146109; CD44; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1
UniProt ID: (Human) Q16064, (Mouse) P15379
Entrez Gene ID: (Human) 960, (Mouse) 12505
cell-matrix adhesion
single organismal cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
hyaluronan catabolic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of heterotypic cell-cell adhesion
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to fibroblast growth factor stimulus
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
cartilage development
interferon-gamma-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of cell growth
branching involved in ureteric bud morphogenesis
wound healing involved in inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
cell adhesion
positive regulation of gene expression
Wnt signaling pathway
cell migration
neuron projection development
positive regulation of neutrophil apoptotic process
macrophage fusion
branching involved in prostate gland morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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