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Invitrogen
CD44 Chimeric Recombinant Rabbit Monoclonal Antibody (Hermes-1)
FIGURE: 1 / 1
CD44 Chimeric Antibody (MA5-48017) in Flow
Flow Cytometry analysis of CD44 in Human activated T lymphocytes using CD44 Chimeric Monoclonal Antibody (Product # MA5-48017). Cells were stained with an isotype control, FITC Chimeric monoclonal antibody (Product # MA5-47752) (panel A), or CD44 Chimeric Monoclonal Antibody (panel B) at a concentration of 1 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analyzed on a flow-cytometer.
Product Details
MA5-48017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Goat,
Human,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Hermes-1
Immunogen
This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074460
Product Specific Information
Specificity: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD44; CD44 antigen; CD44 molecule; CD44 molecule (Indian blood group); CDw44; cell surface glycoprotein CD44; Cell surface glycoprotein CD44 (hyaluronate binding protein); cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44
Gene Aliases: AU023126; AW121933; AW146109; CD44; CD44A; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; METAA; MIC4; Pgp-1; Pgp1; RHAMM
UniProt ID: (Human) Q16064, (Mouse) P15379, (Rat) P26051
Entrez Gene ID: (Human) 960, (Bovine) 281057, (Goat) 102182426, (Rabbit) 100301546, (Sheep) 101113884, (Mouse) 12505, (Rat) 25406
cell-matrix adhesion
single organismal cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
hyaluronan catabolic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of heterotypic cell-cell adhesion
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to fibroblast growth factor stimulus
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
cartilage development
interferon-gamma-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
cytokine-mediated signaling pathway
wound healing, spreading of cells
regulation of lamellipodium morphogenesis
regulation of cell growth
branching involved in ureteric bud morphogenesis
wound healing involved in inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
cell adhesion
positive regulation of gene expression
Wnt signaling pathway
cell migration
neuron projection development
positive regulation of neutrophil apoptotic process
macrophage fusion
branching involved in prostate gland morphogenesis
blood vessel maturation
inflammatory response
response to organic cyclic compound
response to vitamin A
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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