Invitrogen

CD44var (v6) Monoclonal Antibody (9A4), eBioscience™

2 Published Figures

7 References

View all (19) CD44v6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (2)

CD44var (v6) Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 2

CD44var (v6) Antibody (BMS145) in IHC

Nature 2018 - Figure 5 Foxl1 + telocytes provide essential Wnt ligands to the intestinal stem cell compartment (a,b) Wnt pathway activation analyzed by immunohistochemistry for beta catenin (brown). Insets a' and b' at high magnification. (c-j) Immunofluorescence staining of the Wnt targets CyclinD1 and Sox9. (k-n) Stem cell markers Olfm4 (in situ hybridization) and CD44 (immunohistochemistry) one day post induction in ... View More

CD44var (v6) Antibody in Flow Cytometry (Flow)

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Product Details

BMS145

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

9A4

Immunogen

Rec. Mouse CD44var (v6)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597738

Product Specific Information

Description: Specifically recognizes an epitope encoded by exon v6 on the variant portion of murine CD44.

Standard CD44 (CD44s) lacks the entire variable region and is found mainly in cells of lymphohematopoietic origin. The multiple isoforms (designated CD44v; v = variant) contain alternatively spliced exons inserted within the extracellular domain. These isoforms are known as CD44v1, CD44v2, CD44v3, CD44v4, CD44v5, CD44v6, CD44v7, CD44v8, CD44v9, CD44v10, CD44v11.

BMS145 is suitable for ELISA and Western Blot analysis.

Applications Tested: ELISA, Immunohistochemistry, Western Blotting.

Purity: >93%.

Target Information

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization.

Nature communications

Huang X,Nero T,Weerasekera R,Matej KH,Hinbest A,Jiang Z,Lee RF,Wu L,Chak C,Nijjer J,Gibaldi I,Yang H,Gamble N,Ng WL,Malaker SA,Sumigray K,Olson R,Yan J

BMS145 was used in Immunocytochemistry to show how the model biofilm-forming organism Vibrio cholerae uses two adhesins with overlapping but distinct functions to achieve robust adhesion to diverse surfaces.

Thu Apr 13 00:00:00 EDT 2023

Targeting epithelium-expressed sialyl Lewis glycans improves colonic mucosal wound healing and protects against colitis.

JCI insight

Kelm M,Quiros M,Azcutia V,Boerner K,Cummings RD,Nusrat A,Brazil JC,Parkos CA

BMS145 was used in Immunocytochemistry to suggest that sialylated Lewis glycans on CD44v6 represent epithelial targets for improved recovery of intestinal barrier function and restitution of mucosal homeostasis after inflammation or injury.

Thu Jun 18 00:00:00 EDT 2020

Subepithelial telocytes are an important source of Wnts that supports intestinal crypts.

Nature

Shoshkes-Carmel M,Wang YJ,Wangensteen KJ,Tóth B,Kondo A,Massasa EE,Itzkovitz S,Kaestner KH

Published figure using CD44var (v6) monoclonal antibody (Product # BMS145) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2018

TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis.

Cell death and differentiation

Cammareri P,Vincent DF,Hodder MC,Ridgway RA,Murgia C,Nobis M,Campbell AD,Varga J,Huels DJ,Subramani C,Prescott KLH,Nixon C,Hedley A,Barry ST,Greten FR,Inman GJ,Sansom OJ

BMS145 was used in Immunohistochemistry to investigate how activating mutations of Kras or the NF-κB pathway can drive dedifferentiation of intestinal cells lacking Apc.

Sun Oct 01 00:00:00 EDT 2017

Villification in the mouse: Bmp signals control intestinal villus patterning.

Development (Cambridge, England)

Walton KD,Whidden M,Kolterud Å,Shoffner SK,Czerwinski MJ,Kushwaha J,Parmar N,Chandhrasekhar D,Freddo AM,Schnell S,Gumucio DL

BMS145 was used in Immunofluorescence to elucidate the mechanisms driving villus emergence in the murine intestine.

Mon Feb 01 00:00:00 EST 2016

Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma.

Nature immunology

Finkin S,Yuan D,Stein I,Taniguchi K,Weber A,Unger K,Browning JL,Goossens N,Nakagawa S,Gunasekaran G,Schwartz ME,Kobayashi M,Kumada H,Berger M,Pappo O,Rajewsky K,Hoshida Y,Karin M,Heikenwalder M,Ben-Neriah Y,Pikarsky E

BMS145 was used in Immunofluorescence-paraffin to reveal the function of ectopic lymphoid-like structures in cancer.

Tue Dec 01 00:00:00 EST 2015

Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).

The Journal of experimental medicine

Wittig BM,Johansson B,Zöller M,Schwärzler C,Günthert U

Published figure using CD44var (v6) monoclonal antibody (Product # BMS145) in Flow Cytometry

Mon Jun 19 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: AL133330.1; CD44; CD44 antigen; CD44 splice variant; CD44 V6; CD44 var; CD44 variant; CD44v6; CD44var (v6); ECMR-III; ECMRIII; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; HUTCHI; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; MGC10468; MUTCH-I; PGP I; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Gene Aliases: AU023126; AW121933; AW146109; Cd44; HERMES; Ly-24; Pgp-1

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UniProt ID: (Mouse) P15379

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Entrez Gene ID: (Mouse) 12505

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Function(s)

hyalurononglucosaminidase activity cytokine receptor activity epidermal growth factor receptor binding protein binding hyaluronic acid binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD44var (v6) Monoclonal Antibody (9A4), eBioscience™

CD44var (v6) Antibody (BMS145) in IHC

Product Details

BMS145

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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