Invitrogen

CD45 Monoclonal Antibody (2D1), PerCP-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

14 Published Figures

29 References

View all (407) CD45 antibodies

Datasheet

Protocols

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Datasheet

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CD45 Antibody (45-9459-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (blue histogram) or Anti-Human CD45 PerCP-Cyanine5-5 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD45 Antibody — Antibody clone (2D1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 2D1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (2D1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

45-9459-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D1

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548697

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo.

Frontiers in immunology

Oyer JL,Croom-Perez TJ,Dieffenthaller TA,Robles-Carillo LD,Gitto SB,Altomare DA,Copik AJ

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

N⁶-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.

Cancer cell

Cheng Y,Xie W,Pickering BF,Chu KL,Savino AM,Yang X,Luo H,Nguyen DT,Mo S,Barin E,Velleca A,Rohwetter TM,Patel DJ,Jaffrey SR,Kharas MG

45-9459-42 was used in Flow cytometry/Cell sorting to investigate How N6-Methyladenosine dictates its diverse molecular and cellular effects in leukemias.

Mon Jul 12 00:00:00 EDT 2021

High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.

Cell metabolism

Jeong S,Savino AM,Chirayil R,Barin E,Cheng Y,Park SM,Schurer A,Mullarky E,Cantley LC,Kharas MG,Keshari KR

45-9459-42 was used in Flow Cytometry to show targeting the de novo serine synthesis pathway can inhibit fructose metabolism of leukemic cells.

Tue Jan 05 00:00:00 EST 2021

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

45-9459 was used in Flow cytometry/Cell sorting to suggest modifications to the costimulatory domains of CAR-T cells can enable longer persistence and thereby improve antitumor response.

Mon Jun 01 00:00:00 EDT 2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

45-9459 was used in Flow cytometry/Cell sorting to suggest modifications to the costimulatory domains of CAR-T cells can enable longer persistence and thereby improve antitumor response.

Mon Jun 01 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

A transcriptionally and functionally distinct PD-1⁺ CD8⁺ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.

Nature medicine

Thommen DS,Koelzer VH,Herzig P,Roller A,Trefny M,Dimeloe S,Kiialainen A,Hanhart J,Schill C,Hess C,Savic Prince S,Wiese M,Lardinois D,Ho PC,Klein C,Karanikas V,Mertz KD,Schumacher TN,Zippelius A

45-9459 was used in Flow cytometry/Cell sorting to compare transcriptional, metabolic and functional signatures of intratumoral CD8+ T lymphocyte populations with high, intermediate and no PD-1 expression from non-small-cell lung cancer patients.

Sun Jul 01 00:00:00 EDT 2018

Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.

Nature medicine

Zhang B,Nguyen LXT,Li L,Zhao D,Kumar B,Wu H,Lin A,Pellicano F,Hopcroft L,Su YL,Copland M,Holyoake TL,Kuo CJ,Bhatia R,Snyder DS,Ali H,Stein AS,Brewer C,Wang H,McDonald T,Swiderski P,Troadec E,Chen CC,Dorrance A,Pullarkat V,Yuan YC,Perrotti D,Carlesso N,Forman SJ,Kortylewski M,Kuo YH,Marcucci G

45-9459 was used in Flow cytometry/Cell sorting to demonstrate how bone marrow endothelial cells supply miR-126 to leukemia stem cells in chronic myelogenous leukemia which modulates their quiescence and self-renewal.

Tue May 01 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Thu Aug 30 00:00:00 EDT 2018

Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses.

JCI insight

Watanabe K,Luo Y,Da T,Guedan S,Ruella M,Scholler J,Keith B,Young RM,Engels B,Sorsa S,Siurala M,Havunen R,Tähtinen S,Hemminki A,June CH

45-9459 was used in Flow cytometry/Cell sorting to investigate whether combined mesothelin-redirected chimeric antigen receptor T cell therapy with an oncolytic adenovirus expressing TNF-α and IL-2 would improve anti-tumour efficacy.

Thu Apr 05 00:00:00 EDT 2018

The N⁶-methyladenosine (m⁶A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells.

Nature medicine

Vu LP,Pickering BF,Cheng Y,Zaccara S,Nguyen D,Minuesa G,Chou T,Chow A,Saletore Y,MacKay M,Schulman J,Famulare C,Patel M,Klimek VM,Garrett-Bakelman FE,Melnick A,Carroll M,Mason CE,Jaffrey SR,Kharas MG

45-9459 was used in Flow cytometry/Cell sorting to provide a rationale for the therapeutic targeting of METTL3 in myeloid leukemia.

Wed Nov 01 00:00:00 EDT 2017

Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.

Obesity (Silver Spring, Md.)

Subramanian S,Pallati PK,Rai V,Sharma P,Agrawal DK,Nandipati KC

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria.

PLoS pathogens

Yoder AC,Guo K,Dillon SM,Phang T,Lee EJ,Harper MS,Helm K,Kappes JC,Ochsenbauer C,McCarter MD,Wilson CC,Santiago ML

45-9459 was used in Flow cytometry/Cell sorting to profile the transcriptome of primary intestinal CD4+ T cells infected ex vivo with transmitted/founder HIV-1.

Wed Feb 01 00:00:00 EST 2017

Antibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo.

Scientific reports

Liew PX,Kim JH,Lee WY,Kubes P

45-9459 was used in Flow cytometry/Cell sorting to investigate a novel antibody-dependent cell death pathway.

Tue Sep 05 00:00:00 EDT 2017

Overexpression of TLR2 and TLR9 on monocyte subsets of active rheumatoid arthritis patients contributes to enhance responsiveness to TLR agonists.

Arthritis research & therapy

Lacerte P,Brunet A,Egarnes B,Duchêne B,Brown JP,Gosselin J

45-9459 was used in Flow cytometry/Cell sorting to show the presence of EBV genome in monocytes and neutrophils reinforces its role in the exacerbation of rheumatoid arthritis symptoms.

Wed Jan 13 00:00:00 EST 2016

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Thu Nov 17 00:00:00 EST 2016

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Progression of Lung Cancer Is Associated with Increased Dysfunction of T Cells Defined by Coexpression of Multiple Inhibitory Receptors.

Cancer immunology research

Thommen DS,Schreiner J,Müller P,Herzig P,Roller A,Belousov A,Umana P,Pisa P,Klein C,Bacac M,Fischer OS,Moersig W,Savic Prince S,Levitsky V,Karanikas V,Lardinois D,Zippelius A

45-9459 was used in Flow cytometry/Cell sorting to characterise the diversity and expression patterns of inhibitory receptors on tumour-infiltrating T cells from patients with non-small cell lung cancer.

Tue Dec 01 00:00:00 EST 2015

Functional heterogeneity of genetically defined subclones in acute myeloid leukemia.

Cancer cell

Klco JM,Spencer DH,Miller CA,Griffith M,Lamprecht TL,O'Laughlin M,Fronick C,Magrini V,Demeter RT,Fulton RS,Eades WC,Link DC,Graubert TA,Walter MJ,Mardis ER,Dipersio JF,Wilson RK,Ley TJ

45-9459 was used in Flow cytometry/Cell sorting to demonstrate the importance of integrating genetic and functional data in studies of primary cancer samples.

Mon Mar 17 00:00:00 EDT 2014

Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.

Cancer research

Lee CY,Lin Y,Bratman SV,Feng W,Kuo AH,Scheeren FA,Engreitz JM,Varma S,West RB,Diehn M

45-9459 was used in Flow cytometry/Cell sorting to demonstrate a mechanism through which HER2-targeting therapies could benefit a larger number of breast cancer patients.

Wed Jan 01 00:00:00 EST 2014

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD45 monoclonal antibody (Product # 45-9459-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Quantitative reconstruction of leukocyte subsets using DNA methylation.

Genome biology

Accomando WP,Wiencke JK,Houseman EA,Nelson HH,Kelsey KT

45-9459 was used in Flow cytometry/Cell sorting to study how precise immune cell differential estimates can be reconstructed using only DNA rather than whole cells.

Wed Mar 05 00:00:00 EST 2014

Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Ji N,Somanaboeina A,Dixit A,Kawamura K,Hayward NJ,Self C,Olson GL,Forsthuber TG

45-9459 was used in Flow cytometry/Cell sorting to investigate how blocking the HLA-DR2b allele with small molecule inhibitors may offer a promising therapeutic strategy for the treatment of multiple sclerosis.

Fri Nov 15 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (2D1), PerCP-Cyanine5.5, eBioscience™

CD45 Antibody (45-9459-42) in Flow

Product Details

45-9459-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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