Invitrogen

CD45R Monoclonal Antibody (RA3-6B2), APC

FIGURE: 1 / 1

CD45R Antibody (RM2605) in Flow

Fresh mouse splenocytes were stained using APC of rat anti-mouse CD45R (B220) antibody (clone: RA3-6B2). The negative control profiles represent unstained cells.

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Product Details

RM2605

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Immunogen

exon A-specific epitope on CD45

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10392558

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Novel mode of ISG15-mediated protection against influenza A virus and Sendai virus in mice.

Journal of virology

Morales DJ,Monte K,Sun L,Struckhoff JJ,Agapov E,Holtzman MJ,Stappenbeck TS,Lenschow DJ

RM2605 was used in flow cytometry to describe the pathogenesis of influenza A virus and Sendai virus in ISG15 knockout mice

Thu Jan 01 00:00:00 EST 2015

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Immunology letters

Guo Z,Li H,Han M,Xu T,Wu X,Zhuang Y

RM2605 was used in flow cytometry to generate and characterize Id3 conditional T cell knock out mice

Wed Mar 30 00:00:00 EDT 2011

p53 represses class switch recombination to IgG2a through its antioxidant function.

Journal of immunology (Baltimore, Md. : 1950)

Guikema JE,Schrader CE,Brodsky MH,Linehan EK,Richards A,El Falaky N,Li DH,Sluss HK,Szomolanyi-Tsuda E,Stavnezer J

RM2605 was used in Flow cytometry/Cell sorting to demonstrate that the reactive oxygen species scavenger N-acetylcysteine inhibits immunoglobulin class switch recombination.

Tue Jun 01 00:00:00 EDT 2010

Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.

Journal of immunology (Baltimore, Md. : 1950)

Wells JW,Cowled CJ,Farzaneh F,Noble A

RM2605 was used in flow cytometry to identify key signals required for the exponential expansion of specific CD8 positive mouse T cells in vivo

Mon Sep 01 00:00:00 EDT 2008

Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching.

Journal of immunology (Baltimore, Md. : 1950)

Guikema JE,Schrader CE,Leus NG,Ucher A,Linehan EK,Werling U,Edelmann W,Stavnezer J

RM2605 was used in Flow cytometry/Cell sorting to assess the role of Mut S homologue 5 in Ig class switch recombination.

Mon Dec 15 00:00:00 EST 2008

APE1- and APE2-dependent DNA breaks in immunoglobulin class switch recombination.

The Journal of experimental medicine

Guikema JE,Linehan EK,Tsuchimoto D,Nakabeppu Y,Strauss PR,Stavnezer J,Schrader CE

RM2605 was used in Flow cytometry/Cell sorting to study how abasic sites are converted into single-stranded breaks and, subsequently, double-standed breaks.

Mon Nov 26 00:00:00 EST 2007

Antibody class switching differs among SJL, C57BL/6 and 129 mice.

International immunology

Kaminski DA,Stavnezer J

RM2605 was used in flow cytometry to compare Ig class switching in different inbred mice

Sun Apr 01 00:00:00 EDT 2007

TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire.

European journal of immunology

Silver K,Ferry H,Crockford T,Cornall RJ

RM2605 was used in flow cytometry to demonstrate that the effects of Toll-like receptors on autoimmunity occur in the established repertoire and not during B cell development

Thu Jun 01 00:00:00 EDT 2006

Enhanced IgA class switching in marginal zone and B1 B cells relative to follicular/B2 B cells.

Journal of immunology (Baltimore, Md. : 1950)

Kaminski DA,Stavnezer J

RM2605 was used in flow cytometry to investigate IgA production by different B cell subsets

Wed Nov 01 00:00:00 EST 2006

Expansion of bone marrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFN-alpha in NZB bone marrow.

Journal of immunology (Baltimore, Md. : 1950)

Lian ZX,Kikuchi K,Yang GX,Ansari AA,Ikehara S,Gershwin ME

RM2605 was used in flow cytometry to elucidate the mechanism of hyper-IFN-alpha production using New Zealand Black mice

Fri Oct 15 00:00:00 EDT 2004

Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer T cells.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Lan F,Zeng D,Higuchi M,Higgins JP,Strober S

RM2605 was used in flow cytometry to test if a total lymphoid irradiation and anti-thymocyte serum regimen can prevent graft verses host disease.

Sun Jun 01 00:00:00 EDT 2003

Naive B lymphocytes undergo homeostatic proliferation in response to B cell deficit.

Journal of immunology (Baltimore, Md. : 1950)

Cabatingan MS,Schmidt MR,Sen R,Woodland RT

RM2605 was used in flow cytometry to investigate homeostatic B cell proliferation.

Sun Dec 15 00:00:00 EST 2002

An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus.

Journal of immunology (Baltimore, Md. : 1950)

Pan L,Hanrahan J,Li J,Hale LP,Zhuang Y

RM2605 was used in flow cytometry to explore the role of E2A in T cell lineage.

Mon Apr 15 00:00:00 EDT 2002

Allogeneic bone marrow cells that facilitate complete chimerism and eliminate tumor cells express both CD8 and T-cell antigen receptor-alphabeta.

Blood

Lan F,Zeng D,Huie P,Higgins JP,Strober S

RM2605 was used in flow cytometry to test if specific subsets of cells in allogeneic bone marrow transplants can treat the BCL(1) B-cell lymphoma without inducing graft verses host disease.

Fri Jun 01 00:00:00 EDT 2001

Predominance of NK1.1+TCR alpha beta+ or DX5+TCR alpha beta+ T cells in mice conditioned with fractionated lymphoid irradiation protects against graft-versus-host disease: "natural suppressor" cells.

Journal of immunology (Baltimore, Md. : 1950)

Lan F,Zeng D,Higuchi M,Huie P,Higgins JP,Strober S

RM2605 was used in flow cytometry to develop a nonmyeloablative host conditioning regimen for transplantation.

Wed Aug 15 00:00:00 EDT 2001

Immature multipotent hemopoietic progenitors lacking long-term bone marrow-reconstituting activity in the aorta-gonad-mesonephros region of murine day 10 fetuses.

Journal of immunology (Baltimore, Md. : 1950)

Ohmura K,Kawamoto H,Lu M,Ikawa T,Ozaki S,Nakao K,Katsura Y

RM2605 was used in flow cytometry to examine multipotent progenitors from early fetuses.

Thu Mar 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45R Monoclonal Antibody (RA3-6B2), APC

CD45R Antibody (RM2605) in Flow

Product Details

RM2605

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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