Invitrogen

CD45RA Monoclonal Antibody (MEM-56)

CD45RA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD45RA Antibody (MA1-19113) in IHC (P)

Immunohistochemical analysis of CD45RA was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More

CD45RA Antibody in Flow Cytometry (Flow)

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Product Details

MA1-19113

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Flow Cytometry (Flow)

1-4 µg/mL

View 22 publications 22 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Baboon, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MEM-56

Immunogen

Human thymocytes and T lymphocytes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072449

Product Specific Information

This antibody reacts with an extracellular epitope of CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.

This antibody has been successfully used in Mass Cytometry (CyTof).

Immunohistochemistry (Paraffin): No pre-treatment of tissue sections is needed.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells.

Cytotherapy

Ma Q,Garber HR,Lu S,He H,Tallis E,Ding X,Sergeeva A,Wood MS,Dotti G,Salvado B,Ruisaard K,Clise-Dwyer K,John LS,Rezvani K,Alatrash G,Shpall EJ,Molldrem JJ

MA119113 was used in flow cytometry to generate a T-cell receptor-like monoclonal antibody (8F4) that binds the PR1/HLA-A2 complex and test it in preclinical models of acute myeloid leukemia

Mon Aug 01 00:00:00 EDT 2016

Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate.

Vaccine

Graves SF,Kouriba B,Diarra I,Daou M,Niangaly A,Coulibaly D,Keita Y,Laurens MB,Berry AA,Vekemans J,Ripley Ballou W,Lanar DE,Dutta S,Gray Heppner D,Soisson L,Diggs CL,Thera MA,Doumbo OK,Plowe CV,Sztein MB,Lyke KE

MA119113 was used in flow cytometry to characterize the immune responses of children immunized with Plasmodium falciparum apical membrane antigen 1

Tue May 17 00:00:00 EDT 2016

Use of Alefacept for Preconditioning in Multiply Transfused Pediatric Patients with Nonmalignant Diseases.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Stenger EO,Chiang KY,Haight A,Qayed M,Kean L,Horan J

MA1-19113 was used in flow cytometry to report that alefacept depletes both memory T cells and NK cells.

Thu Oct 01 00:00:00 EDT 2015

Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo.

Immunobiology

Kreiser S,Eckhardt J,Kuhnt C,Stein M,Krzyzak L,Seitz C,Tucher C,Knippertz I,Becker C,Günther C,Steinkasserer A,Lechmann M

MA1-19113 was used in flow cytometry to study the role of CD93 molecule in mature dendritic cells and T cells

Sun Feb 01 00:00:00 EST 2015

Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.

PloS one

Grieco AJ,Billett HH,Green NS,Driscoll MC,Bouhassira EE

MA1-19113 was used in flow cytometry to elucidate regulation of γ-globin expression in F-cells.

Mon Feb 29 00:00:00 EST 2016

Impaired survival of regulatory T cells in pulmonary sarcoidosis.

Respiratory research

Broos CE,van Nimwegen M,Kleinjan A,ten Berge B,Muskens F,in 't Veen JC,Annema JT,Lambrecht BN,Hoogsteden HC,Hendriks RW,Kool M,van den Blink B

MA1-19113 was used in flow cytometry to determine the proportions, phenotype, survival, and apoptotic susceptibility of Tregs in sarcoidosis.

Wed Sep 16 00:00:00 EDT 2015

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

MA1-19113 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets

Thu Dec 18 00:00:00 EST 2014

The effects of age and cytomegalovirus on markers of inflammation and lymphocyte populations in captive baboons.

PloS one

Willis EL,Eberle R,Wolf RF,White GL,McFarlane D

MA1-19113 was used in flow cytometry to study the effect of age, gender, social status and peer group on T cells in baboons

Tue Jun 16 00:00:00 EDT 2015

Changes in natural Foxp3(+)Treg but not mucosally-imprinted CD62L(neg)CD38(+)Foxp3(+)Treg in the circulation of celiac disease patients.

PloS one

van Leeuwen MA,du Pré MF,van Wanrooij RL,de Ruiter LF,Raatgeep HR,Lindenbergh-Kortleve DJ,Mulder CJ,de Ridder L,Escher JC,Samsom JN

MA1-19113 was used in flow cytometry to examine Tregs in patients with Celiac disease.

Tue Feb 18 00:00:00 EST 2014

Identification of a human Th1-like IFNγ-secreting Treg subtype deriving from effector T cells.

Journal of autoimmunity

Venigalla RK,Guttikonda PJ,Eckstein V,Ho AD,Sertel S,Lorenz HM,Tretter T

MA1-19113 was used in flow cytometry to characterize and study human IFNγ-producing T regulatory cells.

Sat Dec 01 00:00:00 EST 2012

Functional deficits of pertussis-specific CD4+ T cells in infants compared to adults following DTaP vaccination.

Clinical and experimental immunology

Sharma SK,Pichichero ME

MA1-19113 was used in flow cytometry to determine the function and phenotypes of antigen-specific CD4(+) T cells in adults compared to infants upon acellular pertussis vaccine administration

Sat Sep 01 00:00:00 EDT 2012

IL-21 can supplement suboptimal Lck-independent MAPK activation in a STAT-3-dependent manner in human CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Imataki O,Ansén S,Tanaka M,Butler MO,Berezovskaya A,Milstein MI,Kuzushima K,Nadler LM,Hirano N

MA1-19113 was used in flow cytometry to investigate the role of CD8 and antigen in proliferation of CTLs.

Wed Feb 15 00:00:00 EST 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

MA1-19113 was used in flow cytometry to analyze the regulation of Amphiregulin expression in human T cell subsets

Thu Dec 13 00:00:00 EST 2012

Human TCR transgenic Bet v 1-specific Th1 cells suppress the effector function of Bet v 1-specific Th2 cells.

Journal of immunology (Baltimore, Md. : 1950)

Neunkirchner A,Leb-Reichl VM,Schmetterer KG,Mutschlechner S,Kueng HJ,Haiderer D,Schuch K,Wallner M,Jahn-Schmid B,Bohle B,Pickl WF

MA1-19113 was used in flow cytometry to clone and functionally assess a human αβ TCR specific for the immunodominant epitope of Bet v 1.

Sat Oct 15 00:00:00 EDT 2011

Long-term programming of antigen-specific immunity from gene expression signatures in the PBMC of rhesus macaques immunized with an SIV DNA vaccine.

PloS one

Belisle SE,Yin J,Shedlock DJ,Dai A,Yan J,Hirao L,Kutzler MA,Lewis MG,Andersen H,Lank SM,Karl JA,O'Connor DH,Khan A,Sardesai N,Chang J,Aicher L,Palermo RE,Weiner DB,Katze MG,Boyer J

MA1-19113 was used in flow cytometry to study DNA-based vaccines to treat Rhesus macaques-infected with SIV.

Fri Oct 14 00:00:00 EDT 2011

Polyclonal expansion of human CD4(+)CD25(+) regulatory T cells.

Methods in molecular biology (Clifton, N.J.)

Hoffmann P,Eder R,Edinger M

MA1-19113 was used in flow cytometry to discuss methods for the in vitro expansion of human Treg cells

Wed Feb 02 00:00:00 EST 2011

IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice.

Blood

Markley JC,Sadelain M

MA1-19113 was used in flow cytometry to compare the therapeutic efficacy of CD19-specific human primary T cells that constitutively express gamma(c)-cytokines

Thu Apr 29 00:00:00 EDT 2010

Th17 central memory T cells are reduced by FTY720 in patients with multiple sclerosis.

Neurology

Mehling M,Lindberg R,Raulf F,Kuhle J,Hess C,Kappos L,Brinkmann V

MA1-19113 was used in flow cytometry to determine the effect of FTY720 treatment on Th17 cells

Tue Aug 03 00:00:00 EDT 2010

Upregulation of K2P5.1 potassium channels in multiple sclerosis.

Annals of neurology

Bittner S,Bobak N,Herrmann AM,Göbel K,Meuth P,Höhn KG,Stenner MP,Budde T,Wiendl H,Meuth SG

MA1-19113 was used in flow cytometry to examine the function of K(2P)5.1 in T cells and multiple sclerosis

Thu Jul 01 00:00:00 EDT 2010

Ki-67 staining for determination of rhesus macaque T cell proliferative responses ex vivo.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Shedlock DJ,Talbott KT,Morrow MP,Ferraro B,Hokey DA,Muthumani K,Weiner DB

MA1-19113 was used in flow cytometry to develop a new ex vivo method to study rhesus macaque T cell proliferation

Mon Mar 01 00:00:00 EST 2010

Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation.

Blood

Göbel F,Taschner S,Jurkin J,Konradi S,Vaculik C,Richter S,Kneidinger D,Mühlbacher C,Bieglmayer C,Elbe-Bürger A,Strobl H

MA1-19113 was used in flow cytometry to elucidate factors involved in myeloid dendritic cell differentiation

Thu Oct 29 00:00:00 EDT 2009

Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation.

European journal of immunology

Hoffmann P,Boeld TJ,Eder R,Huehn J,Floess S,Wieczorek G,Olek S,Dietmaier W,Andreesen R,Edinger M

MA1-19113 was used in flow cytometry to report that T regulatory cells lose FOXP3 expression and begin to produce pro-inflammatory cytokines when expanded in vitro

Wed Apr 01 00:00:00 EDT 2009

CD127 and CD25 expression defines CD4+ T cell subsets that are differentially depleted during HIV infection.

Journal of immunology (Baltimore, Md. : 1950)

Dunham RM,Cervasi B,Brenchley JM,Albrecht H,Weintrob A,Sumpter B,Engram J,Gordon S,Klatt NR,Frank I,Sodora DL,Douek DC,Paiardini M,Silvestri G

MA1-19113 was used in flow cytometry to determine how the proportions of CD4(+) T cell subsets change during HIV infection.

Tue Apr 15 00:00:00 EDT 2008

Comparative study of regulatory T cell function of human CD25CD4 T cells from thymocytes, cord blood, and adult peripheral blood.

Clinical & developmental immunology

Fujimaki W,Takahashi N,Ohnuma K,Nagatsu M,Kurosawa H,Yoshida S,Dang NH,Uchiyama T,Morimoto C

MA1-19113 was used in flow cytometry to assess the regulatory function of human CD25+CD4+ T cells at various stages of maturity

Fri Jan 02 00:00:00 EST 2009

In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood.

Nature protocols

Park CY,Majeti R,Weissman IL

MA1-19113 was used in flow cytometry to develop a xenotransplantation system using human hematopoietic stem cells and mice

Wed Apr 01 00:00:00 EDT 2009

The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Jamieson CH,Gotlib J,Durocher JA,Chao MP,Mariappan MR,Lay M,Jones C,Zehnder JL,Lilleberg SL,Weissman IL

MA1-19113 was used in flow cytometry to detect the JAK2 V617F mutation in hematopoietic stem cells and their progeny in patients with polycythemia vera

Tue Apr 18 00:00:00 EDT 2006

Perturbations of cell cycle control in T cells contribute to the different outcomes of simian immunodeficiency virus infection in rhesus macaques and sooty mangabeys.

Journal of virology

Paiardini M,Cervasi B,Sumpter B,McClure HM,Sodora DL,Magnani M,Staprans SI,Piedimonte G,Silvestri G

MA1-19113 was used in flow cytometry to compare cell cycle dysregulation in simian immunodeficiency virus-infected rhesus macaques and sooty mangabeys

Sun Jan 01 00:00:00 EST 2006

Monocyte-derived human macrophages mediate anergy in allogeneic T cells and induce regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Hoves S,Krause SW,Schütz C,Halbritter D,Schölmerich J,Herfarth H,Fleck M

MA1-19113 was used in flow cytometry to assess the T cell-stimulatory capacity of mature dendritic cells and macrophages using a model of allogeneic MLR

Tue Aug 15 00:00:00 EDT 2006

Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Broxmeyer HE,Kim CH

MA1-19113 was used in flow cytometry to report the expression of chemokine receptors on FOXP3+ T cells

Sat Jul 15 00:00:00 EDT 2006

Dynamic differentiation of activated human peripheral blood CD8+ and CD4+ effector memory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Schwendemann J,Choi C,Schirrmacher V,Beckhove P

MA1-19113 was used in flow cytometry to elucidate the lineage of central memory T cells and effector memory T cells

Mon Aug 01 00:00:00 EDT 2005

Varicella-zoster virus transfer to skin by T Cells and modulation of viral replication by epidermal cell interferon-alpha.

The Journal of experimental medicine

Ku CC,Zerboni L,Ito H,Graham BS,Wallace M,Arvin AM

MA1-19113 was used in flow cytometry to investigate T cell-mediated transfer of varicella-zoster virus to skin

Mon Oct 04 00:00:00 EDT 2004

Tropism of varicella-zoster virus for human tonsillar CD4(+) T lymphocytes that express activation, memory, and skin homing markers.

Journal of virology

Ku CC,Padilla JA,Grose C,Butcher EC,Arvin AM

MA1-19113 was used in flow cytometry to report that Varicella-zoster virus infects human tonsillar CD4(+) T cells.

Fri Nov 01 00:00:00 EST 2002

Hyposialated 185 kDa CD45RA+ molecules attain a high concentration in B lymphoma cells and in activated human B cells.

European journal of haematology

Yu Y,Rabinowitz R,Polliack A,Ben-Bassat H,Schlesinger M

MA1-19113 was used in western blot to study CD45 molecules in B cells

Tue Jan 01 00:00:00 EST 2002

A novel mutation in PTPRC interferes with splicing and alters the structure of the human CD45 molecule.

Immunogenetics

Jacobsen M,Hoffmann S,Cepok S,Stei S,Ziegler A,Sommer N,Hemmer B

MA1-19113 was used in flow cytometry to identify a mutation in PTPRCas a cause of variant CD45 expression in humans

Sat Jun 01 00:00:00 EDT 2002

Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.

Blood

Sharron M,Pöhlmann S,Price K,Lolis E,Tsang M,Kirchhoff F,Doms RW,Lee B

MA1-19113 was used in flow cytometry to characterize the localization and function of STRL33/Bonzo.

Sat Jul 01 00:00:00 EDT 2000

Generation of interferon alpha-producing predendritic cell (Pre-DC)2 from human CD34(+) hematopoietic stem cells.

The Journal of experimental medicine

Blom B,Ho S,Antonenko S,Liu YJ

MA1-19113 was used in flow cytometry to determine the developmental pathway of pre-DC2/IPCs.

Mon Dec 18 00:00:00 EST 2000

Normal T-cell turnover in sooty mangabeys harboring active simian immunodeficiency virus infection.

Journal of virology

Chakrabarti LA,Lewin SR,Zhang L,Gettie A,Luckay A,Martin LN,Skulsky E,Ho DD,Cheng-Mayer C,Marx PA

MA1-19113 was used in flow cytometry to compared the effect of SIV infection on T-cell regeneration in sooty mangabeys and rhesus macaques.

Tue Feb 01 00:00:00 EST 2000

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW

MA1-19113 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.

Tue Apr 27 00:00:00 EDT 1999

Increased expression of phosphatidylinositol-specific phospholipase C resistant prion proteins on the surface of activated platelets.

British journal of haematology

Holada K,Mondoro TH,Muller J,Vostal JG

MA1-19113 was used in western blot to investigate the cellular processing of PrP (C) using platelets.

Thu Oct 01 00:00:00 EDT 1998

CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.

Journal of immunology (Baltimore, Md. : 1950)

Kim CH,Pelus LM,White JR,Applebaum E,Johanson K,Broxmeyer HE

MA1-19113 was used in flow cytometry to determine the functional properties of CK beta-11/MIP-3 beta/ELC.

Sun Mar 01 00:00:00 EST 1998

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RA Monoclonal Antibody (MEM-56)

CD45RA Antibody (MA1-19113) in IHC (P)

Product Details

MA1-19113

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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