Invitrogen

CD45RO Monoclonal Antibody (UCHL1)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

31 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

CD45RO Antibody (MA5-11532) in IHC (P)

Formalin-fixed, paraffin-embedded human lymph node stained with CD45RO antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T lymphocytes.

CD45RO Antibody in Flow Cytometry (Flow)

CD45RO Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-11532

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Interleukin-2-dependent human T lymphocytes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.02 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979361

Product Specific Information

MA5-11532 targets CD45RO in WB, FACS and IHC (P) applications and shows reactivity with Human, Mouse, Rat and Non-human primate samples.

The MA5-11532 immunogen is interleukin-2-dependent human T lymphocytes.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A nanofiber-hydrogel composite improves tissue repair in a rat model of Crohn's disease perianal fistulas.

Science advances

Li L,Yao ZC,Parian A,Yang YH,Chao J,Yin J,Salimian KJ,Reddy SK,Zaheer A,Gearhart SL,Mao HQ,Selaru FM

MA5-11532 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that ADSC-loaded mfNHC is a promising therapy for CD-PAF, and warrants further studies to advance mfNHC toward clinical translation.

Wed Jan 04 00:00:00 EST 2023

Lung Inflammation in alpha-1-antitrypsin deficient individuals with normal lung function.

Respiratory research

Kokturk N,Khodayari N,Lascano J,Riley EL,Brantly ML

MA5-11532 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to investigate the characteristics of lower respiratory tract of AATD individuals prior to the onset of clinically significant COPD.

Thu Feb 02 00:00:00 EST 2023

PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.

Nature communications

Liu XD,Kong W,Peterson CB,McGrail DJ,Hoang A,Zhang X,Lam T,Pilie PG,Zhu H,Beckermann KE,Haake SM,Isgandrova S,Martinez-Moczygemba M,Sahni N,Tannir NM,Lin SY,Rathmell WK,Jonasch E

MA5-11532 was used in Immunohistochemistry to investigate if PBRM1/Pbrm1 deficiency can reduce the binding of brahma-related gene 1 (BRG1) to the IFNγ receptor 2 (Ifngr2) promoter, thus decreasing STAT1 phosphorylation and the subsequent expression of IFNγ target genes.

Fri May 01 00:00:00 EDT 2020

PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.

Nature communications

Liu XD,Kong W,Peterson CB,McGrail DJ,Hoang A,Zhang X,Lam T,Pilie PG,Zhu H,Beckermann KE,Haake SM,Isgandrova S,Martinez-Moczygemba M,Sahni N,Tannir NM,Lin SY,Rathmell WK,Jonasch E

Fri May 01 00:00:00 EDT 2020

A case report of clonal EBV-like memory CD4⁺ T cell activation in fatal checkpoint inhibitor-induced encephalitis.

Nature medicine

Johnson DB,McDonnell WJ,Gonzalez-Ericsson PI,Al-Rohil RN,Mobley BC,Salem JE,Wang DY,Sanchez V,Wang Y,Chastain CA,Barker K,Liang Y,Warren S,Beechem JM,Menzies AM,Tio M,Long GV,Cohen JV,Guidon AC,O'Hare M,Chandra S,Chowdhary A,Lebrun-Vignes B,Goldinger SM,Rushing EJ,Buchbinder EI,Mallal SA,Shi C,Xu Y,Moslehi JJ,Sanders ME,Sosman JA,Balko JM

MA5-11532 was used in Immunohistochemistry to identify if CD4+ and CD8+ T cells are potential culprits of checkpoint inhibitor-associated immune encephalitis.

Thu Aug 01 00:00:00 EDT 2019

Nephrogenic Systemic Fibrosis Is Mediated by Myeloid C-C Chemokine Receptor 2.

The Journal of investigative dermatology

Do C,Drel V,Tan C,Lee D,Wagner B

MA5-11532 was used in Immunocytochemistry-immunoflourescence to establish a mouse model of nephrogenic systemic fibrosis.

Tue Oct 01 00:00:00 EDT 2019

Gadolinium-based contrast agents: Stimulators of myeloid-induced renal fibrosis and major metabolic disruptors.

Toxicology and applied pharmacology

Do C,Ford B,Lee DY,Tan C,Escobar P,Wagner B

MA5-11532 was used in Immunohistochemistry to examine the consequences of GBCA treatment in the kidneys from mice with normal kidney function and the potential interplay between obesity and gadolinium exposure.

Mon Jul 15 00:00:00 EDT 2019

The absence of HLA class I expression in non-small cell lung cancer correlates with the tumor tissue structure and the pattern of T cell infiltration.

International journal of cancer

Perea F,Bernal M,Sánchez-Palencia A,Carretero J,Torres C,Bayarri C,Gómez-Morales M,Garrido F,Ruiz-Cabello F

MA511532 was used in immunohistochemistry - frozen section to characterize HLA class I-positive and negative tumors

Wed Feb 15 00:00:00 EST 2017

Trichinella Spiralis Impact on Mesenchymal Stem Cells: Immunohistochemical Study by Image Analyzer in Murine Model.

Experimental and molecular pathology

Hasby Saad MA,Hasby EA

MA511532 was used in immunohistochemistry - paraffin section to test if Trichinella spiralis infection or its derivative stimulate recruitment of CD105+/CD45- cells

Thu Jun 01 00:00:00 EDT 2017

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

MA511532 was used in flow cytometry to define the contribution of CD103+ tumor-infiltrating lymphocytes to high-grade serous epithelial ovarian cancer

Tue Nov 15 00:00:00 EST 2016

Resistance to Antiangiogenic Therapy Is Associated with an Immunosuppressive Tumor Microenvironment in Metastatic Renal Cell Carcinoma.

Cancer immunology research

Liu XD,Hoang A,Zhou L,Kalra S,Yetil A,Sun M,Ding Z,Zhang X,Bai S,German P,Tamboli P,Rao P,Karam JA,Wood C,Matin S,Zurita A,Bex A,Griffioen AW,Gao J,Sharma P,Tannir N,Sircar K,Jonasch E

Published figure using CD45RO monoclonal antibody (Product # MA5-11532) in Immunohistochemistry

Tue Sep 01 00:00:00 EDT 2015

High frequency of CD8 positive lymphocyte infiltration correlates with lack of lymph node involvement in early rectal cancer.

Disease markers

Däster S,Eppenberger-Castori S,Hirt C,Zlobec I,Delko T,Nebiker CA,Soysal SD,Amicarella F,Iezzi G,Sconocchia G,Heberer M,Lugli A,Spagnoli GC,Kettelhack C,Terracciano L,Oertli D,von Holzen U,Tornillo L,Droeser RA

MA5-11532 was used in Immunohistochemistry to determine the efficacy of immunoprofiling in predicting lymph node involvement in early rectal cancer.

Fri Jul 31 00:00:00 EDT 2015

CD30⁺ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Oncology letters

Yang YJ,Li YX,Liu YB,Yang M,Liu K

MA5-11532 was used in immunohistochemistry (paraffin) to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma.

Thu May 01 00:00:00 EDT 2014

A giant primary sclerosing lipogranuloma of the scrotum.

Turk patoloji dergisi

Mungan S,Karagüzel E,Turan C,Reıs A

MA5-11532 was used in immunohistochemistry to report on a case of giant primary sclerosing lipogranuloma of the scrotum

Tue Jul 08 00:00:00 EDT 2014

IL-21 can supplement suboptimal Lck-independent MAPK activation in a STAT-3-dependent manner in human CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Imataki O,Ansén S,Tanaka M,Butler MO,Berezovskaya A,Milstein MI,Kuzushima K,Nadler LM,Hirano N

MA5-11532 was used in flow cytometry to investigate the role of CD8 and antigen in proliferation of CTLs.

Wed Feb 15 00:00:00 EST 2012

The significance of multiplex PCR/heteroduplex analysis-based TCR-γ gene rearrangement combined with laser-capture microdissection in the diagnosis of early mycosis fungoides.

Journal of cutaneous pathology

Yang H,Xu C,Tang Y,Wan C,Liu W,Wang L

MA5-11532 was used in immunohistochemistry (paraffin) to study the diagnostic value for detecting early mycosis fungoides of a combination of multiplex PCR analyis of TCRgamma gene rearrangements and laser-capture microdissection.

Thu Mar 01 00:00:00 EST 2012

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Cytopathology : official journal of the British Society for Clinical Cytology

Su XY,Huang J,Jiang Y,Tang Y,Li GD,Liu WP

MA5-11532 was used in flow cytometry to characterize extranodal natural killer/T-cell lymphoma, nasal type using patient samples.

Sun Apr 01 00:00:00 EDT 2012

Effector Memory T cells Are Associated With Atherosclerosis in Humans and Animal Models.

Journal of the American Heart Association

Ammirati E,Cianflone D,Vecchio V,Banfi M,Vermi AC,De Metrio M,Grigore L,Pellegatta F,Pirillo A,Garlaschelli K,Manfredi AA,Catapano AL,Maseri A,Palini AG,Norata GD

MA5-11532 was used in flow cytometry to study the relationship between circulating CD4+ T-cell subsets and atherosclerosis

Wed Feb 01 00:00:00 EST 2012

Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.

Development (Cambridge, England)

Honma Y,Kawano M,Kohsaka S,Ogawa M

MA5-11532 was used in immunohistochemistry to study the role of Ret in the neurturin-mediated formation of precise axonal projections in the central nervous system

Thu Jul 01 00:00:00 EDT 2010

A patient with clinicopathologic features of small plaque parapsoriasis presenting later with plaque-stage mycosis fungoides: report of a case and comparative retrospective study of 27 cases of "nonprogressive" small plaque parapsoriasis.

Journal of the American Academy of Dermatology

Belousova IE,Vanecek T,Samtsov AV,Michal M,Kazakov DV

MA5-11532 was used in immunohistochemistry to report on a case of small plaque parapsoriasis with plaque-stage mycosis fungoides

Mon Sep 01 00:00:00 EDT 2008

Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas.

The American journal of surgical pathology

Kazakov DV,Belousova IE,Kacerovska D,Sima R,Vanecek T,Vazmitel M,Pizinger K,Michal M

MA5-11532 was used in immunohistochemistry to study 53 cases of cutaneous lymphoproliferative disorders

Wed Oct 01 00:00:00 EDT 2008

Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis.

Clinical and experimental immunology

Yates J,Whittington A,Mitchell P,Lechler RI,Lightstone L,Lombardi G

MA5-11532 was used in flow cytometry to examine the frequency, phenotype, and function of T regulatory cells in patients with systemic lupus erythematosus and inactive disease

Tue Jul 01 00:00:00 EDT 2008

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Cancer research

Carolan BJ,Heguy A,Harvey BG,Leopold PL,Ferris B,Crystal RG

MA5-11532 was used in immunocytochemistry to investigate the expression of carboxyl-terminal hydrolyase L1 gene in human airway epithelium of cigarette smokers

Wed Nov 15 00:00:00 EST 2006

Monocyte-derived human macrophages mediate anergy in allogeneic T cells and induce regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Hoves S,Krause SW,Schütz C,Halbritter D,Schölmerich J,Herfarth H,Fleck M

MA5-11532 was used in flow cytometry to assess the T cell-stimulatory capacity of mature dendritic cells and macrophages using a model of allogeneic MLR

Tue Aug 15 00:00:00 EDT 2006

Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Journal of cutaneous pathology

Boudova L,Kazakov DV,Jindra P,Sima R,Vanecek T,Kuntscher V,Vera V,Bouda J,Michal M

MA5-11532 was used in immunohistochemistry to report on a case of utaneous lymphoma of the head co-expressing CD56 and CD30

Tue Aug 01 00:00:00 EDT 2006

CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R.

World journal of gastroenterology

Zhang YS,Yuan FJ,Jia GF,Zhang JF,Hu LY,Huang L,Wang J,Dai ZQ

MA5-11532 was used in immunohistochemistry to investigate the effectiveness of cytokine-induced killer cell treatment in patient with hepatocellular carcinoma

Tue Jun 14 00:00:00 EDT 2005

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA5-11532 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

T-cell-rich B-cell lymphoma: a clinicopathologic study of 21 cases and comparison with 43 cases of diffuse large B-cell lymphoma.

Leukemia research

Aki H,Tuzuner N,Ongoren S,Baslar Z,Soysal T,Ferhanoglu B,Sahinler I,Aydin Y,Ulku B,Aktuglu G

MA5-11532 was used in immunohistochemistry to compare the clinocopathology of T-cell rich B-cell lymphoma and diffuse large B-cell lymphoma

Mon Mar 01 00:00:00 EST 2004

Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.

Journal of immunology (Baltimore, Md. : 1950)

Thompson SD,Luyrink LK,Graham TB,Tsoras M,Ryan M,Passo MH,Glass DN

MA5-11532 was used in flow cytometry to elucidate the mechanisms that promote recruitment and survival of T cells within the pediatric inflamed joint.

Fri Jun 01 00:00:00 EDT 2001

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW

MA5-11532 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.

Tue Apr 27 00:00:00 EDT 1999

CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.

Journal of immunology (Baltimore, Md. : 1950)

Kim CH,Pelus LM,White JR,Applebaum E,Johanson K,Broxmeyer HE

MA5-11532 was used in flow cytometry to determine the functional properties of CK beta-11/MIP-3 beta/ELC.

Sun Mar 01 00:00:00 EST 1998

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Immunology

Smith SH,Brown MH,Rowe D,Callard RE,Beverley PC

MA5-11532 was used in flow cytometry, immunohistochemistry - frozen section, and immunoprecipitation to characterize the monoclonal antibody UCHL1.

Thu May 01 00:00:00 EDT 1986

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; RT7; T200

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UniProt ID: (Human) P08575, (Mouse) P06800, (Rat) P04157

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Entrez Gene ID: (Human) 5788, (Mouse) 19264, (Rat) 24699

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein kinase regulator activity protein phosphatase protein modifying enzyme extracellular matrix protein

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

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CD45RO Monoclonal Antibody (UCHL1)

CD45RO Antibody (MA5-11532) in IHC (P)

Product Details

MA5-11532

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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