Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 8
CD46 Antibody (PA5-78985) in ICC/IF
Immunocytochemistry analysis of CD46 using anti-CD46 antibody (Product # PA5-78985) . CD46 was detected in a section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-CD46 antibody (Product # PA5-78985) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visu... View More
Product Details
PA5-78985
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD46 (366-392aa YRYLQRRKKKGTYLTDETHREVKFTSL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746101
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody TRA-2-10; CD46; CD46 antigen, complement regulatory protein; CD46 molecule, complement regulatory protein; complement membrane cofactor protein; measles virus receptor; Membrane cofactor protein; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MGC26544; TLX; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen
Gene Aliases: AHUS2; CD46; MCP; MIC10; TLX; TRA2.10
UniProt ID: (Human) P15529, (Mouse) O88174, (Rat) Q9Z0M4
Entrez Gene ID: (Human) 4179, (Mouse) 17221, (Rat) 29333
T cell mediated immunity
proteolysis
single fertilization
regulation of Notch signaling pathway
positive regulation of gene expression
negative regulation of gene expression
interleukin-10 production
positive regulation of interleukin-10 production
sequestering of extracellular ligand from receptor
positive regulation of T cell proliferation
negative regulation of catalytic activity
positive regulation of memory T cell differentiation
positive regulation of regulatory T cell differentiation
negative regulation of complement activation
positive regulation of transforming growth factor beta production
adaptive immune response
complement activation, classical pathway
regulation of complement activation
innate immune response
viral entry into host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support