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Invitrogen
CD46 Monoclonal Antibody (8E2), PerCP-eFluor™ 710, eBioscience™
Product Details
46-0469-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
8E2
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573682
Product Specific Information
Description: The 8E2 monoclonal antibody reacts with human CD46 (membrane cofactor protein). CD46 is a transmembrane glycoprotein consisting of four regularly expressed isoforms, giving an electrophoretic profile of two heterogeneous protein species of 51-58 kDa ("lower band") and 59-68 kDa ("upper band"). CD46 is expressed on the majority of nucleated cells, and functions as a regulator of complement function by binding complement components C3b and C4b, which serves to protect the host from autologous complement attack. CD46 has been called a "pathogen magnet" as it is known to play a role in the entry of several pathogens including strains of measles virus, human herpes virus type 6, group A streptococci and Neisseria.
Cross-linking TCR on naive human peripheral blood CD4+ T cells together with CD46, rather than CD28, induces more robust proliferation than achieved with stimulation by the costimulatory molecule CD28, as well as other qualitative differences. There is evidence to suggest that stimulation of TCR and CD46 activates natural CD4+CD25+ T regs, which are non-proliferating, but produce high amounts of granzyme A, and kill activated CD4+, CD8+, CD14+ monocytes, immature dendritic cells and mature dendritic cells.
Applications Reported: This 8E2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 8E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody TRA-2-10; CD46; CD46 antigen, complement regulatory protein; CD46 molecule, complement regulatory protein; complement membrane cofactor protein; measles virus receptor; Membrane cofactor protein; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MGC26544; TLX; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen
Gene Aliases: AHUS2; CD46; MCP; MIC10; TLX; TRA2.10
UniProt ID: (Human) P15529
Entrez Gene ID: (Human) 4179
adaptive immune response
T cell mediated immunity
proteolysis
complement activation, classical pathway
single fertilization
regulation of Notch signaling pathway
positive regulation of gene expression
negative regulation of gene expression
regulation of complement activation
interleukin-10 production
positive regulation of interleukin-10 production
sequestering of extracellular ligand from receptor
positive regulation of T cell proliferation
negative regulation of catalytic activity
positive regulation of memory T cell differentiation
innate immune response
positive regulation of regulatory T cell differentiation
negative regulation of complement activation
viral entry into host cell
positive regulation of transforming growth factor beta production
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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