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CD48 Antibody (MA5-38105) in WB
Western blot analysis of CD48 in various lysates. Samples were incubated with CD48 Monoclonal antibody (Product # MA5-38105) using a dilution of 1:20,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-38105
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1B6W3
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-220 of human CD48 (NP_0017692)
Conjugate
Form
Liquid
Concentration
2.50 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2898023
Product Specific Information
Positive Samples: Raji
Immunogen sequence: QGHLVHMTVV SGSNVTLNIS ESLPENYKQL TWFYTFDQKI VEWDSRKSKY FESKFKGRVR LDPQSGALYI SKVQKEDNST YIMRVLKKTG NEQEWKIKLQ VLDPVPKPVI KIEKIEDMDD NCYLKLSCVI PGESVNYTWY GDKRPFPKEL QNSVLETTLM PHNYSRCYTC QVSNSVSSKN GTVCLSPPCT LARS
Target Information
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell activation marker; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; BLAST-1; CD48; CD48 antigen; CD48 antigen (B cell membrane protein); CD48 antigen (B-cell membrane protein); CD48d; HM48-1; Leukocyte antigen MEM-102; MRC OX-45 surface antigen; sCD 48; sCD48; sgp-60; Signaling lymphocytic activation molecule 2; Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2; SLAMF2; soluble CD 48; soluble CD48; TCT.1
Gene Aliases: AI449234; AW610730; Bcm-1; BCM1; BLAST; BLAST-1; BLAST1; CD48; hCD48; mCD48; MEM-102; Sgp-60; SLAMF2
UniProt ID: (Human) P09326, (Mouse) P18181
Entrez Gene ID: (Human) 962, (Mouse) 12506
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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