Invitrogen

CD48 Monoclonal Antibody (HM48-1), FITC, eBioscience™

FIGURE: 1 / 14

CD48 Antibody (11-0481-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD48 FITC (purple histogram). Total viable cells were used for analysis.

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Product Details

11-0481-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HM48-1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465077

Product Specific Information

Description: The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen; also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells (HSC) are highly purified as CD150(+)CD244(-)CD48(-) cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244(+)CD150(-)CD48(-) and the most restricted progenitors are CD48(+)CD244(+)CD150(-). CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo.

Applications Reported: This HM48-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM48-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Deletion of CD36 exhibits limited impact on normal hematopoiesis and the leukemia microenvironment.

Cellular & molecular biology letters

Meng Y,Pospiech M,Ali A,Chandwani R,Vergel M,Onyemaechi S,Yaghmour G,Lu R,Alachkar H

11-0481-82 was used in Flow cytometry/Cell sorting to investigate whether targeting CD36 has any detrimental impact on normal hematopoietic cells.

Wed May 24 00:00:00 EDT 2023

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

11-0481-82 was used in Flow cytometry/Cell sorting to demonstrate that Phf6R274X plays a critical role in fine-tuning T cells and HSC homeostasis.

Fri Jun 16 00:00:00 EDT 2023

Nociceptive nerves regulate haematopoietic stem cell mobilization.

Nature

Gao X,Zhang D,Xu C,Li H,Caron KM,Frenette PS

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Conditionally pathogenic genetic variants of a hematopoietic disease-suppressing enhancer.

Science advances

Soukup AA,Matson DR,Liu P,Johnson KD,Bresnick EH

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Fri Dec 10 00:00:00 EST 2021

MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells.

Nature communications

Wei Q,Pinho S,Dong S,Pierce H,Li H,Nakahara F,Xu J,Xu C,Boulais PE,Zhang D,Maryanovich M,Cuervo AM,Frenette PS

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

p53-dependent induction of P2X7 on hematopoietic stem and progenitor cells regulates hematopoietic response to genotoxic stress.

Cell death & disease

Tung LT,Wang H,Belle JI,Petrov JC,Langlais D,Nijnik A

11-0481-82 was used in Flow Cytometry to analyze the in vivo transcriptional response of hematopoietic stem and progenitor cells to genotoxic stress in a mouse whole-body irradiation model and characterize the p53-dependent and p53-independent branches of this transcriptional response.

Fri Oct 08 00:00:00 EDT 2021

Adaptive response to inflammation contributes to sustained myelopoiesis and confers a competitive advantage in myelodysplastic syndrome HSCs.

Nature immunology

Muto T,Walker CS,Choi K,Hueneman K,Smith MA,Gul Z,Garcia-Manero G,Ma A,Zheng Y,Starczynowski DT

11-0481 was used in Flow cytometry/Cell sorting to show that chronic inflammation is a determinant for the competitive advantage of myelodysplastic syndrome (MDS) hematopoietic stem and progenitor cells (HSPCs) and for disease progression.

Fri May 01 00:00:00 EDT 2020

Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency.

Cell stem cell

Liang R,Arif T,Kalmykova S,Kasianov A,Lin M,Menon V,Qiu J,Bernitz JM,Moore K,Lin F,Benson DL,Tzavaras N,Mahajan M,Papatsenko D,Ghaffari S

11-0481-82 was used in Flow Cytometry to investigate the overall metabolic properties of hematopoietic stem cells.

Thu Mar 05 00:00:00 EST 2020

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

11-0481-82 was used in Immunohistochemistry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis.

Cell reports

Chen C,Sun MA,Warzecha C,Bachu M,Dey A,Wu T,Adams PD,Macfarlan T,Love P,Ozato K

11-0481 was used in Flow cytometry/Cell sorting to show that HIRA provides a chromatin environment essential for HSCs, thereby steering their development and survival.

Tue Feb 18 00:00:00 EST 2020

Ptpn21 Controls Hematopoietic Stem Cell Homeostasis and Biomechanics.

Cell stem cell

Ni F,Yu WM,Wang X,Fay ME,Young KM,Qiu Y,Lam WA,Sulchek TA,Cheng T,Scadden DT,Qu CK

11-0481 was used in Flow cytometry/Cell sorting to show that Ptpn21 maintains cellular mechanics, which is correlated with its important functions in HSC niche retention and preservation of hematopoietic regeneration capacity.

Thu Apr 04 00:00:00 EDT 2019

Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer.

The Journal of clinical investigation

Soukup AA,Zheng Y,Mehta C,Wu J,Liu P,Cao M,Hofmann I,Zhou Y,Zhang J,Johnson KD,Choi K,Keles S,Bresnick EH

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Classification of mouse B cell types using surfaceome proteotype maps.

Nature communications

van Oostrum M,Müller M,Klein F,Bruderer R,Zhang H,Pedrioli PGA,Reiter L,Tsapogas P,Rolink A,Wollscheid B

11-0481 was used in Flow cytometry/Cell sorting to develop population-specific surfaceome proteotype maps to classify developing B cell types in mice.

Mon Dec 16 00:00:00 EST 2019

PPM1D Mutations Drive Clonal Hematopoiesis in Response to Cytotoxic Chemotherapy.

Cell stem cell

Hsu JI,Dayaram T,Tovy A,De Braekeleer E,Jeong M,Wang F,Zhang J,Heffernan TP,Gera S,Kovacs JJ,Marszalek JR,Bristow C,Yan Y,Garcia-Manero G,Kantarjian H,Vassiliou G,Futreal PA,Donehower LA,Takahashi K,Goodell MA

11-0481 was used in Flow cytometry/Cell sorting to establish the clinical relevance of PPM1D mutations in clonal haematopoiesis and the importance of studying mutation-treatment interactions.

Thu Nov 01 00:00:00 EDT 2018

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia.

Developmental cell

Hewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH

11-0481 was used in Flow cytometry/Cell sorting to propose that Samd14-Enh and an ensemble of anemia-responsive enhancers are essential for erythrocyte regeneration in stress erythropoiesis.

Mon Aug 07 00:00:00 EDT 2017

Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

Tue Dec 12 00:00:00 EST 2017

Integrating Enhancer Mechanisms to Establish a Hierarchical Blood Development Program.

Cell reports

Mehta C,Johnson KD,Gao X,Ong IM,Katsumura KR,McIver SC,Ranheim EA,Bresnick EH

Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

MLL-AF9- and HOXA9-mediated acute myeloid leukemia stem cell self-renewal requires JMJD1C.

The Journal of clinical investigation

Zhu N,Chen M,Eng R,DeJong J,Sinha AU,Rahnamay NF,Koche R,Al-Shahrour F,Minehart JC,Chen CW,Deshpande AJ,Xu H,Chu SH,Ebert BL,Roeder RG,Armstrong SA

11-0481 was used in Flow cytometry/Cell sorting to show that loss of JMJD1C substantially decreased LSC frequency and caused differentiation of MLL-AF9- and homeobox A9-driven leukemias.

Tue Mar 01 00:00:00 EST 2016

Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance.

Cell death and differentiation

Belle JI,Petrov JC,Langlais D,Robert F,Cencic R,Shen S,Pelletier J,Gros P,Nijnik A

11-0481 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the transcriptional activation of p53 stress responses in Mysm1-deficient haematopoietic stem and progenitor cells.

Sun May 01 00:00:00 EDT 2016

Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton.

eLife

Yue R,Shen B,Morrison SJ

11-0481 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts.

Tue Dec 13 00:00:00 EST 2016

Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors.

Nature communications

Cheng H,Ang HY,A El Farran C,Li P,Fang HT,Liu TM,Kong SL,Chin ML,Ling WY,Lim EK,Li H,Huber T,Loh KM,Loh YH,Lim B

11-0481 was used in Flow cytometry/Cell sorting to demonstrate how it is possible to reprogram mouse embryonic fibroblasts into engraftable blood progenitors.

Mon Nov 21 00:00:00 EST 2016

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

11-0481 was used in Flow cytometry/Cell sorting to illustrate a paradigm in which cis-elements in a locus differentially control stem and progenitor cell transitions.

Tue Sep 01 00:00:00 EDT 2015

High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice.

Stem cell research

Rao TN,Marks-Bluth J,Sullivan J,Gupta MK,Chandrakanthan V,Fitch SR,Ottersbach K,Jang YC,Piao X,Kulkarni RN,Serwold T,Pimanda JE,Wagers AJ

11-0481 was used in Flow cytometry/Cell sorting to indicate that high-level expression of GPR56 by primitive HSPCs is largely dispensable for steady-state and regenerative hematopoiesis.

Fri May 01 00:00:00 EDT 2015

Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma.

Nature communications

Lee CL,Castle KD,Moding EJ,Blum JM,Williams N,Luo L,Ma Y,Borst LB,Kim Y,Kirsch DG

11-0481 was used in Flow cytometry/Cell sorting to show that total-body irradiation promotes the expansion of a rare population of thymocytes that express oncogenic Kras(G12D).

Thu Sep 24 00:00:00 EDT 2015

Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity.

The Journal of clinical investigation

Johnson KD,Hsu AP,Ryu MJ,Wang J,Gao X,Boyer ME,Liu Y,Lee Y,Calvo KR,Keles S,Zhang J,Holland SM,Bresnick EH

11-0481 was used in Flow cytometry/Cell sorting to identify a novel mutation in a GATA2 cis-element which causes deficits in haematopoietic stem/progenitor activity.

Mon Oct 01 00:00:00 EDT 2012

ZBTB1 is a determinant of lymphoid development.

The Journal of experimental medicine

Siggs OM,Li X,Xia Y,Beutler B

11-0481 was used in Flow cytometry/Cell sorting to identify a mutation causative of complete and cell-intrinsic T cell deficiency.

Mon Jan 16 00:00:00 EST 2012

Loss of Cxcl12/Sdf-1 in adult mice decreases the quiescent state of hematopoietic stem/progenitor cells and alters the pattern of hematopoietic regeneration after myelosuppression.

Blood

Tzeng YS,Li H,Kang YL,Chen WC,Cheng WC,Lai DM

11-0481 was used in Immunofluorescence to elucidate the function of the chemokine, Cxcl12, in adult hematopoietic stem/progenitor cell (HSPC) regulation.

Thu Jan 13 00:00:00 EST 2011

Defective hematopoietic stem cell and lymphoid progenitor development in the Ts65Dn mouse model of Down syndrome: potential role of oxidative stress.

Antioxidants & redox signaling

Lorenzo LP,Chen H,Shatynski KE,Clark S,Yuan R,Harrison DE,Yarowsky PJ,Williams MS

11-0481 was used in Flow cytometry/Cell sorting to identify a link between oxidative stress and diminished IL-7Rα expression and function associated with defective haematologic development in a DS mouse model.

Sat Oct 15 00:00:00 EDT 2011

Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells.

Blood

Tolar J,Ishida-Yamamoto A,Riddle M,McElmurry RT,Osborn M,Xia L,Lund T,Slattery C,Uitto J,Christiano AM,Wagner JE,Blazar BR

11-0481 was used in Flow cytometry/Cell sorting to investigate whether bone marrow cell infusion can be used to treat disease in a murine model of recessive dystrophic epidermolysis bullosa.

Thu Jan 29 00:00:00 EST 2009

Bioinformatics

Protein Aliases: B-cell activation marker; BCM1 surface antigen; BLAST-1; CD48; CD48 antigen; CD48 antigen (B cell membrane protein); CD48d; HM48-1; MRC OX-45 surface antigen; sCD 48; sCD48; sgp-60; Signaling lymphocytic activation molecule 2; Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2; SLAMF2; soluble CD 48; soluble CD48

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Gene Aliases: AI449234; AW610730; Bcm-1; BCM1; BLAST; BLAST-1; BLAST1; Cd48; MEM-102; Sgp-60; SLAMF2

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UniProt ID: (Mouse) P18181

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Entrez Gene ID: (Mouse) 12506

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Function(s)

antigen binding receptor activity protein binding immunoglobulin receptor superfamily

Process(es)

signal transduction T cell activation mast cell activation

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CD48 Monoclonal Antibody (HM48-1), FITC, eBioscience™

CD48 Antibody (11-0481-82) in Flow

Product Details

11-0481-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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