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Invitrogen
CD49f Recombinant Rabbit Monoclonal Antibody (5H14L18)
FIGURE: 1 / 2
CD49f Antibody (701178) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane1), MDCK (Lane2), CaCo-2 (Lane 3) and A549 (Lane4). The blots were probed with Integrin alpha 6 Rabbit Monoclonal Antibody (Product # 701178, 2-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 150 kDa band corresponding to Integrin alpha 6 was observed across cell lines tested. Known quantity of protein samples were elect... View More
Product Details
701178
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H14L18
Immunogen
Recombinant protein corresponding to amino acids 102-299 of human Integrin alpha 6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532422
Product Specific Information
This antibody is predicted to react with equine, rabbit and rat based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6
Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6
UniProt ID: (Human) P23229, (Mouse) Q61739
Entrez Gene ID: (Human) 3655, (Dog) 100856563, (Mouse) 16403
cell-substrate junction assembly
cell-matrix adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
positive regulation of cell-cell adhesion
extracellular matrix organization
hemidesmosome assembly
cell-substrate adhesion
cellular response to extracellular stimulus
cell adhesion mediated by integrin
nail development
positive regulation of phosphorylation
positive regulation of apoptotic process
positive regulation of GTPase activity
skin development
positive regulation of transcription from RNA polymerase II promoter
filopodium assembly
digestive tract development
brown fat cell differentiation
leukocyte migration
renal system development
amelogenesis
cell-cell adhesion
negative regulation of extrinsic apoptotic signaling pathway
cell adhesion
positive regulation of cell-substrate adhesion
single organismal cell-cell adhesion
odontogenesis of dentin-containing tooth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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