Invitrogen

CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), PE, eBioscience™

CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 52

CD49f (Integrin alpha 6) Antibody (12-0495-82) in Flow

Staining of normal human peripheral blood cells with Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (blue histogram) or Anti-Human/Mouse CD49f (Integrin alpha 6) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD49f (Integrin alpha 6) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

12-0495-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/test

View 75 publications 75 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Cat, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioGoH3 (GoH3)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891474

Product Specific Information

Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

12-0495-82 was used in Flow Cytometry to find that HSA was beneficial to maintaining CD34+ cell expansion and short-term colony formation in vitro and optimizing multilineage reconstitution in immunodeficient mice in vivo.

Tue Jan 31 00:00:00 EST 2023

Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium.

Conservation physiology

Wang DH,Wu XM,Chen JS,Cai ZG,An JH,Zhang MY,Li Y,Li FP,Hou R,Liu YL

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2022

Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis.

Cell reports

Wei X,Zhang L,Zhang Y,Cooper C,Brewer C,Tsai CF,Wang YT,Glaz M,Wessells HB,Que J,Titus MA,Cirulli V,Glaser A,Liu T,Reder NP,Creighton CJ,Xin L

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

ROR2 regulates self-renewal and maintenance of hair follicle stem cells.

Nature communications

Veltri A,Lang CMR,Cangiotti G,Chan CK,Lien WH

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression.

Nature communications

Li N,Liu Q,Han Y,Pei S,Cheng B,Xu J,Miao X,Pan Q,Wang H,Guo J,Wang X,Zhang G,Lian Y,Zhang W,Zang Y,Tan M,Li Q,Wang X,Xiao Y,Hu G,Jiang J,Huang H,Qin J

12-0495-82 was used in flow cytometry to show that ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, functions downstream of inflammation-induced IKKβ activation to shape the immunosuppressive tumor microenvironment (TME).

Sat Nov 26 00:00:00 EST 2022

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota.

Cell

Lima-Junior DS,Krishnamurthy SR,Bouladoux N,Collins N,Han SJ,Chen EY,Constantinides MG,Link VM,Lim AI,Enamorado M,Cataisson C,Gil L,Rao I,Farley TK,Koroleva G,Attig J,Yuspa SH,Fischbach MA,Kassiotis G,Belkaid Y

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Thu Jul 08 00:00:00 EDT 2021

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI insight

Kashgari G,Venkatesh S,Refuerzo S,Pham B,Bayat A,Klein RH,Ramos R,Ta AP,Plikus MV,Wang PH,Andersen B

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

Generation and functional characterization of murine mammary organoids.

STAR protocols

Yip HYK,Papa A

12-0495-82 was used in Flow Cytometry to describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway.

Fri Sep 17 00:00:00 EDT 2021

Generation and functional characterization of murine mammary organoids.

STAR protocols

Yip HYK,Papa A

Fri Sep 17 00:00:00 EDT 2021

Developmental genetics of color pattern establishment in cats.

Nature communications

Kaelin CB,McGowan KA,Barsh GS

12-0495-82 was used in Functional assays to identify when, where, and how, during fetal development, felid color patterns are established.

Tue Sep 07 00:00:00 EDT 2021

Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

Science advances

Abu El Maaty MA,Grelet E,Keime C,Rerra AI,Gantzer J,Emprou C,Terzic J,Lutzing R,Bornert JM,Laverny G,Metzger D

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

Science advances

Abu El Maaty MA,Grelet E,Keime C,Rerra AI,Gantzer J,Emprou C,Terzic J,Lutzing R,Bornert JM,Laverny G,Metzger D

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.

Cell

Shwartz Y,Gonzalez-Celeiro M,Chen CL,Pasolli HA,Sheu SH,Fan SM,Shamsi F,Assaad S,Lin ET,Zhang B,Tsai PC,He M,Tseng YH,Lin SJ,Hsu YC

12-0495-82 was used in Flow Cytometry to demonstrate sympathetic nerves can modulate stem cells through synapse-like connections and neurotransmitters to couple tissue production with demands.

Thu Aug 06 00:00:00 EDT 2020

Infiltrating Mast Cell-Mediated Stimulation of Estrogen Receptor Activity in Breast Cancer Cells Promotes the Luminal Phenotype.

Cancer research

Majorini MT,Cancila V,Rigoni A,Botti L,Dugo M,Triulzi T,De Cecco L,Fontanella E,Jachetti E,Tagliabue E,Chiodoni C,Tripodo C,Colombo MP,Lecis D

12-0495-82 was used in Flow Cytometry to support the notion that MCs influence the phenotype of breast cancer cells by stimulating a luminal phenotype and ultimately modifying the outcome of the disease.

Mon Jun 01 00:00:00 EDT 2020

A genetically defined disease model reveals that urothelial cells can initiate divergent bladder cancer phenotypes.

Proceedings of the National Academy of Sciences of the United States of America

Wang L,Smith BA,Balanis NG,Tsai BL,Nguyen K,Cheng MW,Obusan MB,Esedebe FN,Patel SJ,Zhang H,Clark PM,Sisk AE,Said JW,Huang J,Graeber TG,Witte ON,Chin AI,Park JW

12-0495 was used in Flow cytometry/Cell sorting to reveal distinct molecular features of small cell carcinoma of the bladder and provide a transcriptional dataset and a preclinical model for further investigating SCCB biology.

Tue Jan 07 00:00:00 EST 2020

An IFT20 mechanotrafficking axis is required for integrin recycling, focal adhesion dynamics, and polarized cell migration.

Molecular biology of the cell

Su S,Begum S,Ezratty EJ

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Tang C,Span PN,Rowan AE,Aalders TW,Schalken JA,Adema GJ,Kouwer PHJ,Zegers MMP,Ansems M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

12-0495 was used in Flow cytometry/Cell sorting to identify distinct populations of isotype-switched memory B lymphocytes of murine spleen and bone marrow.

Fri May 22 00:00:00 EDT 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

12-0495 was used in Flow cytometry/Cell sorting to identify distinct populations of isotype-switched memory B lymphocytes of murine spleen and bone marrow.

Fri May 22 00:00:00 EDT 2020

Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC.

Scientific reports

Li H,Chaitankar V,Zhu J,Chin K,Liu W,Pirooznia M,Rodgers GP

12-0495-82 was used in Flow Cytometry to indicate that OLFM4 is co-expressed with multiple stem/progenitor cell marker genes in prostate epithelial cells and acts as a novel mediator in prostate stem/progenitor cell proliferation and differentiation.

Mon Dec 14 00:00:00 EST 2020

Integrated Single-Cell Transcriptomics and Chromatin Accessibility Analysis Reveals Regulators of Mammary Epithelial Cell Identity.

Cell reports

Pervolarakis N,Nguyen QH,Williams J,Gong Y,Gutierrez G,Sun P,Jhutty D,Zheng GXY,Nemec CM,Dai X,Watanabe K,Kessenbrock K

12-0495-82 was used in Flow Cytometry to provide a resource to reveal cis/trans-regulatory elements associated with MEC identity and differentiation that will serve as a reference to determine how the chromatin accessibility landscape changes during breast cancer.

Tue Oct 20 00:00:00 EDT 2020

m6A RNA methylation impacts fate choices during skin morphogenesis.

eLife

Xi L,Carroll T,Matos I,Luo JD,Polak L,Pasolli HA,Jaffrey SR,Fuchs E

12-0495 was used in Flow cytometry/Cell sorting to study mouse skin embryogenesis to tackle m6A's functions and physiological importance.

Wed Aug 26 00:00:00 EDT 2020

Cytosine-5 RNA methylation links protein synthesis to cell metabolism.

PLoS biology

Gkatza NA,Castro C,Harvey RF,Heiß M,Popis MC,Blanco S,Bornelöv S,Sajini AA,Gleeson JG,Griffin JL,West JA,Kellner S,Willis AE,Dietmann S,Frye M

12-0495 was used in Flow cytometry/Cell sorting to study whether changes in tRNA methylation profiles are sufficient to specify cellular metabolic states and efficiently adapt protein synthesis rates to cell stress.

Sat Jun 01 00:00:00 EDT 2019

The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor.

Disease models & mechanisms

Pfefferle AD,Darr DB,Calhoun BC,Mott KR,Rosen JM,Perou CM

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Fri Jul 05 00:00:00 EDT 2019

A basal-enriched microRNA is required for prostate tumorigenesis in a Pten knockout mouse model.

Molecular carcinogenesis

Fan X,Bjerke GA,Riemondy K,Wang L,Yi R

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.

Cells

Rémy M,Ferraro F,Le Salver P,Rey S,Genot E,Djavaheri-Mergny M,Thébaud N,Boiziau C,Boeuf H

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Thu Nov 21 00:00:00 EST 2019

Blimp1⁺ cells generate functional mouse sebaceous gland organoids in vitro.

Nature communications

Feldman A,Mukha D,Maor II,Sedov E,Koren E,Yosefzon Y,Shlomi T,Fuchs Y

12-0495-82 was used in Flow cytometry/Cell sorting to demonstrate that single Blimp1+ cells have the potential to generate SG organoids in vitro.

Tue May 28 00:00:00 EDT 2019

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

12-0495-82 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Early lineage segregation of multipotent embryonic mammary gland progenitors.

Nature cell biology

Wuidart A,Sifrim A,Fioramonti M,Matsumura S,Brisebarre A,Brown D,Centonze A,Dannau A,Dubois C,Van Keymeulen A,Voet T,Blanpain C

12-0495 was used in Flow cytometry/Cell sorting to study the timing and mechanisms mediating early lineage segregation of multipotent progenitors during mammary gland development.

Fri Jun 01 00:00:00 EDT 2018

Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth.

The EMBO journal

Reichenbach B,Classon J,Aida T,Tanaka K,Genander M,Göritz C

12-0495-82 was used in Flow Cytometry to reveal that stem/progenitor cell activation is synchronized over distinct niches during growth and identify SLC1A3 as a general marker and effector of activated epithelial stem/progenitor cells throughout the skin.

Wed May 02 00:00:00 EDT 2018

Integrin-Rac signalling for mammary epithelial stem cell self-renewal.

Breast cancer research : BCR

Olabi S,Ucar A,Brennan K,Streuli CH

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Wed May 23 00:00:00 EDT 2018

Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer.

Cell reports

Barros-Silva JD,Linn DE,Steiner I,Guo G,Ali A,Pakula H,Ashton G,Peset I,Brown M,Clarke NW,Bronson RT,Yuan GC,Orkin SH,Li Z,Baena E

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.

Nature

Naik S,Larsen SB,Gomez NC,Alaverdyan K,Sendoel A,Yuan S,Polak L,Kulukian A,Chai S,Fuchs E

12-0495 was used in Flow cytometry/Cell sorting to study the prolonged memory to acute inflammation that enables mouse epithelial stem cells to hasten barrier restoration after subsequent tissue damage.

Thu Oct 26 00:00:00 EDT 2017

Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.

Cell host & microbe

Zhu F,Willette-Brown J,Song NY,Lomada D,Song Y,Xue L,Gray Z,Zhao Z,Davis SR,Sun Z,Zhang P,Wu X,Zhan Q,Richie ER,Hu Y

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Estrogen modulates mesenchyme-epidermis interactions in the adult nipple.

Development (Cambridge, England)

Wu HJ,Oh JW,Spandau DF,Tholpady S,Diaz J,Schroeder LJ,Offutt CD,Glick AB,Plikus MV,Koyama S,Foley J

12-0495 was used in Flow cytometry/Cell sorting to show that specialized nipple epidermis is maintained by estrogen-induced repression of TGFβ signalling in the local fibroblasts.

Sat Apr 15 00:00:00 EDT 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Induced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.

Nature communications

Tao L,Xiang D,Xie Y,Bronson RT,Li Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Defining stem cell dynamics and migration during wound healing in mouse skin epidermis.

Nature communications

Aragona M,Dekoninck S,Rulands S,Lenglez S,Mascré G,Simons BD,Blanpain C

12-0495 was used in Flow cytometry/Cell sorting to show implications for tissue regeneration, acute and chronic wound disorders.

Wed Mar 01 00:00:00 EST 2017

Stem cell function and stress response are controlled by protein synthesis.

Nature

Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M

12-0495 was used in Flow cytometry/Cell sorting to study how stem cells can revoke translation inhibition pathways to control their function and stress response..

Thu Jun 16 00:00:00 EDT 2016

Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.

Oncotarget

Duru N,Gernapudi R,Lo PK,Yao Y,Wolfson B,Zhang Y,Zhou Q

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2016

Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.

Oncotarget

Rane JK,Erb HH,Nappo G,Mann VM,Simms MS,Collins AT,Visakorpi T,Maitland NJ

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.

Nature communications

Yosef R,Pilpel N,Tokarsky-Amiel R,Biran A,Ovadya Y,Cohen S,Vadai E,Dassa L,Shahar E,Condiotti R,Ben-Porath I,Krizhanovsky V

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Wed Apr 06 00:00:00 EDT 2016

Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.

Oncotarget

Sun JG,Chen XW,Zhang LP,Wang J,Diehn M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion.

Skeletal muscle

Penton CM,Badarinarayana V,Prisco J,Powers E,Pincus M,Allen RE,August PR

12-0495 was used in Flow cytometry/Cell sorting to demonstrate laminin 521 is a superior substrate for both short-term and long-term myogenic cell culture applications compared to other commonly utilized substrates.

Tue Dec 13 00:00:00 EST 2016

Mouse dental pulp stem cells support human umbilical cord blood-derived hematopoietic stem/progenitor cells in vitro.

Cell transplantation

Nakatsuka R,Matsuoka Y,Uemura Y,Sumide K,Iwaki R,Takahashi M,Fujioka T,Sasaki Y,Sonoda Y

12-0495 was used in Flow cytometry/Cell sorting to demonstrate that prospectively isolated murine PDGFRα and Sca-1 double-positive dental pulp stem cells could support primitive human CD34+ and CD34- hematopoietic stem cells in vitro.

Wed Sep 16 00:00:00 EDT 2015

Characterization of autoimmune inflammation induced prostate stem cell expansion.

The Prostate

Wang HH,Wang L,Jerde TJ,Chan BD,Savran CA,Burcham GN,Crist S,Ratliff TL

Thu Oct 01 00:00:00 EDT 2015

The rod domain is not essential for the function of plectin in maintaining tissue integrity.

Molecular biology of the cell

Ketema M,Secades P,Kreft M,Nahidiazar L,Janssen H,Jalink K,de Pereda JM,Sonnenberg A

12-0495 was used in Immunofluorescence to demonstrate that rodless plectin can functionally compensate for the loss of full-length plectin in a knock-in mouse model.

Wed Jul 01 00:00:00 EDT 2015

The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.

The Journal of biological chemistry

Shahi P,Slorach EM,Wang CY,Chou J,Lu A,Ruderisch A,Werb Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Fri Feb 06 00:00:00 EST 2015

Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.

PloS one

Zoch A,Mayerl S,Schulz A,Greither T,Frappart L,Rübsam J,Heuer H,Giovannini M,Morrison H

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon May 23 00:00:00 EDT 2016

Spatially restricted Hedgehog signalling regulates HGF-induced branching of the adult prostate.

Nature cell biology

Lim A,Shin K,Zhao C,Kawano S,Beachy PA

12-0495 was used in Flow cytometry/Cell sorting to study how spatially restricting Hedgehog signalling can regulate HGF-induced branching of the adult prostate.

Mon Dec 01 00:00:00 EST 2014

In vivo transcriptional governance of hair follicle stem cells by canonical Wnt regulators.

Nature cell biology

Lien WH,Polak L,Lin M,Lay K,Zheng D,Fuchs E

12-0495 was used in Flow cytometry/Cell sorting to show that during hair follicle stem cell quiescence, TCF3, TCF4 and TLE bind coordinately and repress Wnt target genes.

Sat Feb 01 00:00:00 EST 2014

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

Mislocalization of the cell polarity protein scribble promotes mammary tumorigenesis and is associated with basal breast cancer.

Cancer research

Feigin ME,Akshinthala SD,Araki K,Rosenberg AZ,Muthuswamy LB,Martin B,Lehmann BD,Berman HK,Pietenpol JA,Cardiff RD,Muthuswamy SK

12-0495 was used in Flow cytometry/Cell sorting to report that SCRIB mRNA is overexpressed and that SCRIB protein is found away from cell-cell junctions in human breast cancers.

Sun Jun 01 00:00:00 EDT 2014

FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.

Development (Cambridge, England)

Zhang X,Martinez D,Koledova Z,Qiao G,Streuli CH,Lu P

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Prolactin-induced prostate tumorigenesis links sustained Stat5 signaling with the amplification of basal/stem cells and emergence of putative luminal progenitors.

The American journal of pathology

Sackmann-Sala L,Chiche A,Mosquera-Garrote N,Boutillon F,Cordier C,Pourmir I,Pascual-Mathey L,Kessal K,Pigat N,Camparo P,Goffin V

12-0495 was used in Flow cytometry/Cell sorting to provide a deeper understanding of the initial stages of prostate tumorigenesis induced by prolactin.

Sat Nov 01 00:00:00 EDT 2014

Impact of prostate inflammation on lesion development in the POET3(+)Pten(+/-) mouse model of prostate carcinogenesis.

The American journal of pathology

Burcham GN,Cresswell GM,Snyder PW,Chen L,Liu X,Crist SA,Henry MD,Ratliff TL

12-0495 was used in Flow cytometry/Cell sorting to study the link between prostatitis and prostate cancer development using an inducible mouse model of prostatitis.

Mon Dec 01 00:00:00 EST 2014

DNA methylation of E-cadherin is a priming mechanism for prostate development.

Developmental biology

Keil KP,Abler LL,Mehta V,Altmann HM,Laporta J,Plisch EH,Suresh M,Hernandez LL,Vezina CM

12-0495 was used in Flow cytometry/Cell sorting to investigate the parallels between the signalling during prostate cancer development and prostate organogenesis.

Sat Mar 15 00:00:00 EDT 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Dec 08 00:00:00 EST 2014

Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues.

Molecular cancer research : MCR

Smith DA,Kiba A,Zong Y,Witte ON

12-0495 was used in Flow cytometry/Cell sorting to indicate that pro-inflammatory cytokines can activate pathways associated with prostate cancer progression, and synergize with cell-autonomous oncogenic events to promote malignancy.

Tue Oct 01 00:00:00 EDT 2013

Leptin receptor maintains cancer stem-like properties in triple negative breast cancer cells.

Endocrine-related cancer

Zheng Q,Banaszak L,Fracci S,Basali D,Dunlap SM,Hursting SD,Rich JN,Hjlemeland AB,Vasanji A,Berger NA,Lathia JD,Reizes O

12-0495 was used in Flow cytometry/Cell sorting to explore the role of the leptin receptor in the maintenance of cancer stem cells.

Sun Dec 01 00:00:00 EST 2013

Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.

Genesis (New York, N.Y. : 2000)

Shiura H,Ikeda R,Lee J,Sato T,Ogonuki N,Hirose M,Ogura A,Ogawa T,Abe K

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.

PloS one

Mukhopadhyay C,Zhao X,Maroni D,Band V,Naramura M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Fri Jul 11 00:00:00 EDT 2014

Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.

PloS one

Bohr S,Patel SJ,Vasko R,Shen K,Huang G,Yarmush ML,Berthiaume F

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Mon Dec 23 00:00:00 EST 2013

Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.

PloS one

Turaç G,Hindley CJ,Thomas R,Davis JA,Deleidi M,Gasser T,Karaöz E,Pruszak J

12-0495 was used in Flow cytometry/Cell sorting to develop a validated protocol enabling the co-detection and co-expression of surface molecules on neural cell populations.

Tue Feb 04 00:00:00 EST 2014

Tumorigenic cells are common in mouse MPNSTs but their frequency depends upon tumor genotype and assay conditions.

Cancer cell

Buchstaller J,McKeever PE,Morrison SJ

12-0495 was used in Flow cytometry/Cell sorting to determine the frequency of malignant peripheral nerve sheath tumour (MPNST) cells with tumourigenic potential in mouse models of MPNST.

Tue Feb 14 00:00:00 EST 2012

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 12-0495-82) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2012

The Ras-like protein R-Ras2/TC21 is important for proper mammary gland development.

Molecular biology of the cell

Larive RM,Abad A,Cardaba CM,Hernández T,Cañamero M,de Álava E,Santos E,Alarcón B,Bustelo XR

12-0495 was used in Flow cytometry/Cell sorting to investigate the function of the GTPase, R-Ras2/TC21, in vivo.

Fri Jun 01 00:00:00 EDT 2012

Purification and direct transformation of epithelial progenitor cells from primary human prostate.

Nature protocols

Goldstein AS,Drake JM,Burnes DL,Finley DS,Zhang H,Reiter RE,Huang J,Witte ON

12-0495 was used in Flow cytometry/Cell sorting to describe a system to directly transform freshly isolated primary human prostate epithelial cells without previous culture or immortalisation.

Sun May 01 00:00:00 EDT 2011

Neural Wiskott-Aldrich syndrome protein modulates Wnt signaling and is required for hair follicle cycling in mice.

The Journal of clinical investigation

Lyubimova A,Garber JJ,Upadhyay G,Sharov A,Anastasoaie F,Yajnik V,Cotsarelis G,Dotto GP,Botchkarev V,Snapper SB

12-0495 was used in Flow cytometry/Cell sorting to propose that N-WASP acts as a positive regulator of beta-catenin-dependent transcription, modulating differentiation of hair follicle progenitor cells.

Mon Feb 01 00:00:00 EST 2010

Isolation, cultivation and characterization of adult murine prostate stem cells.

Nature protocols

Lukacs RU,Goldstein AS,Lawson DA,Cheng D,Witte ON

12-0495 was used in Flow cytometry/Cell sorting to develop a protocol to isolate, cultivate and characterise adult murine prostate stem cells, allowing further studies in organ replenishment and their role in cancer initiation.

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6

View more View less

Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6

View more View less

UniProt ID: (Human) P23229, (Mouse) Q61739

View more View less

Entrez Gene ID: (Human) 3655, (Mouse) 16403

View more View less

Function(s)

integrin binding protein binding insulin-like growth factor I binding neuregulin binding metal ion binding cadherin binding involved in cell-cell adhesion protein complex binding laminin binding integrin

Process(es)

cell-substrate junction assembly cell-matrix adhesion integrin-mediated signaling pathway ectodermal cell differentiation positive regulation of cell-cell adhesion extracellular matrix organization hemidesmosome assembly cell-substrate adhesion cellular response to extracellular stimulus cell adhesion mediated by integrin nail development positive regulation of phosphorylation positive regulation of apoptotic process positive regulation of GTPase activity skin development positive regulation of transcription from RNA polymerase II promoter filopodium assembly digestive tract development brown fat cell differentiation leukocyte migration renal system development amelogenesis cell-cell adhesion negative regulation of extrinsic apoptotic signaling pathway cell adhesion positive regulation of cell-substrate adhesion single organismal cell-cell adhesion odontogenesis of dentin-containing tooth

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), PE, eBioscience™

CD49f (Integrin alpha 6) Antibody (12-0495-82) in Flow

Product Details

12-0495-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!