Invitrogen

CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor™ 450, eBioscience™

CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)

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CD49f (Integrin alpha 6) Antibody (48-0495-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.06 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) eFluor® 450 (right). Total viable cells were used for analysis.

CD49f (Integrin alpha 6) Antibody in Immunohistochemistry (IHC)

CD49f (Integrin alpha 6) Antibody in Immunocytochemistry (ICC/IF)

CD49f (Integrin alpha 6) Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

48-0495-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 46 publications 46 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioGoH3 (GoH3)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042564

Product Specific Information

Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Integrin expression and extracellular matrix adhesion of septoclasts, pericytes, and endothelial cells at the chondro-osseous junction and the metaphysis of the proximal tibia in young mice.

Journal of anatomy

Bando Y,Nagasaka A,Onozawa G,Sakiyama K,Owada Y,Amano O

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunohistochemistry

Mon May 01 00:00:00 EDT 2023

Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection.

Biomaterials

Kim HK,Kim H,Lee MK,Choi WH,Jang Y,Shin JS,Park JY,Bae DH,Hyun SI,Kim KH,Han HW,Lim B,Choi G,Kim M,Chang Lim Y,Yoo J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium.

Conservation physiology

Wang DH,Wu XM,Chen JS,Cai ZG,An JH,Zhang MY,Li Y,Li FP,Hou R,Liu YL

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2022

Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis.

Cell reports

Wei X,Zhang L,Zhang Y,Cooper C,Brewer C,Tsai CF,Wang YT,Glaz M,Wessells HB,Que J,Titus MA,Cirulli V,Glaser A,Liu T,Reder NP,Creighton CJ,Xin L

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

48-0495-82 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 EDT 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

48-0495-82 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 EDT 2022

Embigin is a fibronectin receptor that affects sebaceous gland differentiation and metabolism.

Developmental cell

Sipilä K,Rognoni E,Jokinen J,Tewary M,Vietri Rudan M,Talvi S,Jokinen V,Dahlström KM,Liakath-Ali K,Mobasseri A,Du-Harpur X,Käpylä J,Nutt SL,Salminen TA,Heino J,Watt FM

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunohistochemistry

Mon Jun 20 00:00:00 EDT 2022

ROR2 regulates self-renewal and maintenance of hair follicle stem cells.

Nature communications

Veltri A,Lang CMR,Cangiotti G,Chan CK,Lien WH

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota.

Cell

Lima-Junior DS,Krishnamurthy SR,Bouladoux N,Collins N,Han SJ,Chen EY,Constantinides MG,Link VM,Lim AI,Enamorado M,Cataisson C,Gil L,Rao I,Farley TK,Koroleva G,Attig J,Yuspa SH,Fischbach MA,Kassiotis G,Belkaid Y

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Thu Jul 08 00:00:00 EDT 2021

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

48-0495 was used in Flow cytometry/Cell sorting to determine that canonical Wnt signaling was aberrantly activated in HaCaT cells transfected with lentivirus expressing Piwil2 and in cervical lesion specimens of low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and invasive carcinoma.

Fri Jan 01 00:00:00 EST 2021

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

Fri Jan 01 00:00:00 EST 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI insight

Kashgari G,Venkatesh S,Refuerzo S,Pham B,Bayat A,Klein RH,Ramos R,Ta AP,Plikus MV,Wang PH,Andersen B

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

Generation and functional characterization of murine mammary organoids.

STAR protocols

Yip HYK,Papa A

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunocytochemistry

Fri Sep 17 00:00:00 EDT 2021

Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

Science advances

Abu El Maaty MA,Grelet E,Keime C,Rerra AI,Gantzer J,Emprou C,Terzic J,Lutzing R,Bornert JM,Laverny G,Metzger D

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity.

Cell stem cell

Gupta R,Turati V,Brian D,Thrussel C,Wilbourn B,May G,Enver T

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Neutralization

Thu Apr 02 00:00:00 EDT 2020

An IFT20 mechanotrafficking axis is required for integrin recycling, focal adhesion dynamics, and polarized cell migration.

Molecular biology of the cell

Su S,Begum S,Ezratty EJ

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Tang C,Span PN,Rowan AE,Aalders TW,Schalken JA,Adema GJ,Kouwer PHJ,Zegers MMP,Ansems M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Fri May 22 00:00:00 EDT 2020

Evolutionarily conserved sequence motif analysis guides development of chemically defined hydrogels for therapeutic vascularization.

Science advances

Jia J,Jeon EJ,Li M,Richards DJ,Lee S,Jung Y,Barrs RW,Coyle R,Li X,Chou JC,Yost MJ,Gerecht S,Cho SW,Mei Y

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Neutralization

Wed Jul 01 00:00:00 EDT 2020

The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor.

Disease models & mechanisms

Pfefferle AD,Darr DB,Calhoun BC,Mott KR,Rosen JM,Perou CM

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Fri Jul 05 00:00:00 EDT 2019

A basal-enriched microRNA is required for prostate tumorigenesis in a Pten knockout mouse model.

Molecular carcinogenesis

Fan X,Bjerke GA,Riemondy K,Wang L,Yi R

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Integrin-Rac signalling for mammary epithelial stem cell self-renewal.

Breast cancer research : BCR

Olabi S,Ucar A,Brennan K,Streuli CH

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Wed May 23 00:00:00 EDT 2018

Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer.

Cell reports

Barros-Silva JD,Linn DE,Steiner I,Guo G,Ali A,Pakula H,Ashton G,Peset I,Brown M,Clarke NW,Bronson RT,Yuan GC,Orkin SH,Li Z,Baena E

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.

Nature

Naik S,Larsen SB,Gomez NC,Alaverdyan K,Sendoel A,Yuan S,Polak L,Kulukian A,Chai S,Fuchs E

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Thu Oct 26 00:00:00 EDT 2017

Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.

Cell host & microbe

Zhu F,Willette-Brown J,Song NY,Lomada D,Song Y,Xue L,Gray Z,Zhao Z,Davis SR,Sun Z,Zhang P,Wu X,Zhan Q,Richie ER,Hu Y

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Distinct signaling programs control human hematopoietic stem cell survival and proliferation.

Blood

Knapp DJ,Hammond CA,Aghaeepour N,Miller PH,Pellacani D,Beer PA,Sachs K,Qiao W,Wang W,Humphries RK,Sauvageau G,Zandstra PW,Bendall SC,Nolan GP,Hansen C,Eaves CJ

48-0495 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms by which distinct signalling programs control human hematopoietic stem cell survival and proliferation.

Thu Jan 19 00:00:00 EST 2017

Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny.

The EMBO journal

Chacón-Martínez CA,Klose M,Niemann C,Glauche I,Wickström SA

48-0495 was used in Flow cytometry/Cell sorting to uncovers regulatory dynamics by which phenotypic plasticity of cells drives population-level homeostasis within a niche, and provides a discovery tool for studies on adult stem cell fate.

Tue Jan 17 00:00:00 EST 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Induced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.

Nature communications

Tao L,Xiang D,Xie Y,Bronson RT,Li Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Stem cell function and stress response are controlled by protein synthesis.

Nature

Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M

48-0495 was used in Flow cytometry/Cell sorting to study how stem cells can revoke translation inhibition pathways to control their function and stress response..

Thu Jun 16 00:00:00 EDT 2016

Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation.

Nature cell biology

Welte T,Kim IS,Tian L,Gao X,Wang H,Li J,Holdman XB,Herschkowitz JI,Pond A,Xie G,Kurley S,Nguyen T,Liao L,Dobrolecki LE,Pang L,Mo Q,Edwards DP,Huang S,Xin L,Xu J,Li Y,Lewis MT,Wang T,Westbrook TF,Rosen JM,Zhang XH

48049582 was used in flow cytometry to describe the role of mTOR signalling in recruiting pro-tumorigenic myeloid-derived suppressor cells.

Wed Jun 01 00:00:00 EDT 2016

Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.

Oncotarget

Duru N,Gernapudi R,Lo PK,Yao Y,Wolfson B,Zhang Y,Zhou Q

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2016

Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.

Oncotarget

Rane JK,Erb HH,Nappo G,Mann VM,Simms MS,Collins AT,Visakorpi T,Maitland NJ

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.

Nature communications

Yosef R,Pilpel N,Tokarsky-Amiel R,Biran A,Ovadya Y,Cohen S,Vadai E,Dassa L,Shahar E,Condiotti R,Ben-Porath I,Krizhanovsky V

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Wed Apr 06 00:00:00 EDT 2016

Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.

Oncotarget

Sun JG,Chen XW,Zhang LP,Wang J,Diehn M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

Epidermal keratinocytes initiate wound healing and pro-inflammatory immune responses following percutaneous schistosome infection.

International journal for parasitology

Bourke CD,Prendergast CT,Sanin DE,Oulton TE,Hall RJ,Mountford AP

48-0495 was used in Flow cytometry/Cell sorting to indicate that S.mansoni cercariae and their excretory/secretory products act directly upon epidermal keratinocytes.

Sun Mar 01 00:00:00 EST 2015

The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.

The Journal of biological chemistry

Shahi P,Slorach EM,Wang CY,Chou J,Lu A,Ruderisch A,Werb Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Fri Feb 06 00:00:00 EST 2015

Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.

PloS one

Zoch A,Mayerl S,Schulz A,Greither T,Frappart L,Rübsam J,Heuer H,Giovannini M,Morrison H

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon May 23 00:00:00 EDT 2016

Integrin-linked kinase regulates the niche of quiescent epidermal stem cells.

Nature communications

Morgner J,Ghatak S,Jakobi T,Dieterich C,Aumailley M,Wickström SA

48-0495 was used in Flow cytometry/Cell sorting to uncover a critical role for the basement membrane niche in regulating stem cell activation and thereby skin homeostasis.

Tue Sep 08 00:00:00 EDT 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.

Development (Cambridge, England)

Zhang X,Martinez D,Koledova Z,Qiao G,Streuli CH,Lu P

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunofluorescence

Mon Dec 08 00:00:00 EST 2014

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Immunofluorescence

Mon Dec 08 00:00:00 EST 2014

Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.

Genesis (New York, N.Y. : 2000)

Shiura H,Ikeda R,Lee J,Sato T,Ogonuki N,Hirose M,Ogura A,Ogawa T,Abe K

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.

PloS one

Mukhopadhyay C,Zhao X,Maroni D,Band V,Naramura M

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Fri Jul 11 00:00:00 EDT 2014

Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.

PloS one

Bohr S,Patel SJ,Vasko R,Shen K,Huang G,Yarmush ML,Berthiaume F

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Mon Dec 23 00:00:00 EST 2013

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD49f (Integrin alpha 6) monoclonal antibody (Product # 48-0495-82) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6

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Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6

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UniProt ID: (Human) P23229, (Mouse) Q61739

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Entrez Gene ID: (Human) 3655, (Mouse) 16403

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Function(s)

integrin binding protein binding insulin-like growth factor I binding neuregulin binding metal ion binding cadherin binding involved in cell-cell adhesion protein complex binding laminin binding integrin

Process(es)

cell-substrate junction assembly cell-matrix adhesion integrin-mediated signaling pathway ectodermal cell differentiation positive regulation of cell-cell adhesion extracellular matrix organization hemidesmosome assembly cell-substrate adhesion cellular response to extracellular stimulus cell adhesion mediated by integrin nail development positive regulation of phosphorylation positive regulation of apoptotic process positive regulation of GTPase activity skin development positive regulation of transcription from RNA polymerase II promoter filopodium assembly digestive tract development brown fat cell differentiation leukocyte migration renal system development amelogenesis cell-cell adhesion negative regulation of extrinsic apoptotic signaling pathway cell adhesion positive regulation of cell-substrate adhesion single organismal cell-cell adhesion odontogenesis of dentin-containing tooth

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CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor™ 450, eBioscience™

CD49f (Integrin alpha 6) Antibody (48-0495-82) in Flow

Product Details

48-0495-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Type

Clone

Conjugate

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