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Invitrogen
CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Super Bright™ 780, eBioscience™
FIGURE: 1 / 21
CD49f (Integrin alpha 6) Antibody (78-0495-42) in Flow
Normal human peripheral blood cells cells were stained with CD3 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-0036-82) and Rat IgG2a kappa Isotype Control, Super Bright 780 (Product # 78-4321-82) (left) or CD49f Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
78-0495-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioGoH3 (GoH3)
Conjugate
Excitation/Emission Max
413/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744906
Product Specific Information
Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.
Applications Reported: This eBioGoH3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioGoH3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6
Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6
UniProt ID: (Human) P23229, (Mouse) Q61739
Entrez Gene ID: (Human) 3655, (Mouse) 16403
cell-substrate junction assembly
cell-matrix adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
positive regulation of cell-cell adhesion
extracellular matrix organization
hemidesmosome assembly
cell-substrate adhesion
cellular response to extracellular stimulus
cell adhesion mediated by integrin
nail development
positive regulation of phosphorylation
positive regulation of apoptotic process
positive regulation of GTPase activity
skin development
positive regulation of transcription from RNA polymerase II promoter
filopodium assembly
digestive tract development
brown fat cell differentiation
leukocyte migration
renal system development
amelogenesis
cell-cell adhesion
negative regulation of extrinsic apoptotic signaling pathway
cell adhesion
positive regulation of cell-substrate adhesion
single organismal cell-cell adhesion
odontogenesis of dentin-containing tooth
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