Invitrogen

CD5 Monoclonal Antibody (53-7.3), eFluor™ 450, eBioscience™

FIGURE: 1 / 10

CD5 Antibody (48-0051-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 (Product # 47-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.06 µg of Anti-Mouse CD5 eFluor® 450 (right). Total viable cells were used for analysis.

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Product Details

48-0051-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-7.3

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603250

Product Specific Information

Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.

Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cissus quadrangularis (Hadjod) Inhibits RANKL-Induced Osteoclastogenesis and Augments Bone Health in an Estrogen-Deficient Preclinical Model of Osteoporosis Via Modulating the Host Osteoimmune System.

Cells

Azam Z,Sapra L,Baghel K,Sinha N,Gupta RK,Soni V,Saini C,Mishra PK,Srivastava RK

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Wed Jan 04 00:00:00 EST 2023

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

A novel ZsGreen knock-in melanoma cell line reveals the function of CD11b in tumor phagocytosis.

Immunology and cell biology

Zhang L,Feng X,Shen Y,Wang Y,Liu Z,Ma Y,Gu Y,Guo G,Duan L,Lu L,Liang Y,Lawrence T,Huang R

48-0051-82 was used in Immunohistochemistry to demonstrate that the ZsGreen-expressing YUMM1.7 melanoma model provides a valuable tool for the study of phagocytosis in vivo.

Sat Oct 01 00:00:00 EDT 2022

cDC1 coordinate innate and adaptive responses in the omentum required for T cell priming and memory.

Science immunology

Christian DA,Adams TA,Shallberg LA,Phan AT,Smith TE,Abraha M,Perry J,Ruthel G,Clark JT,Pritchard GH,Aronson LR,Gossa S,McGavern DB,Kedl RM,Hunter CA

48-0051-82 was used in Flow Cytometry to investigate how conventional type 1 dendritic cells (cDC1s) in the peritoneal cavity's omentum coordinate innate responses to microbial challenge and provide secondary signals required for CD8+ T cell expansion and memory formation, particularly after intraperitoneal immunization with Toxoplasma gondii.

Fri Sep 30 00:00:00 EDT 2022

The Influence of B Cell Depletion Therapy on Naturally Acquired Immunity to Streptococcus pneumoniae.

Frontiers in immunology

Ercoli G,Ramos-Sevillano E,Nakajima R,de Assis RR,Jasinskas A,Goldblatt D,Felgner P,Weckbecker G,Brown J

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Tue Jun 22 00:00:00 EDT 2021

β4GALT1 controls β1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis.

Nature communications

Giannini S,Lee-Sundlov MM,Rivadeneyra L,Di Buduo CA,Burns R,Lau JT,Falet H,Balduini A,Hoffmeister KM

48-0051 was used in Flow cytometry/Cell sorting to identify the conserved B4galt1 gene as a critical regulator of thrombopoiesis in megakaryocytes.

Fri Jan 17 00:00:00 EST 2020

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

48-0051 was used in Flow cytometry/Cell sorting to study the activation of Bcl11b, which controls T-cell fate commitment.

Tue Nov 20 00:00:00 EST 2018

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2017

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

48-0051 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

Naïve helper T cells with high CD5 expression have increased calcium signaling.

PloS one

Freitas CMT,Hamblin GJ,Raymond CM,Weber KS

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2017

Mast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile.

Arthritis research & therapy

van der Velden D,Lagraauw HM,Wezel A,Launay P,Kuiper J,Huizinga TW,Toes RE,Bot I,Stoop JN

48-0051 was used in Flow cytometry/Cell sorting to suggest that mast cells may play a role in the regulation of the adaptive immune response during the development of arthritis.

Mon Jun 13 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Efficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI.

Infection and immunity

Dickinson GS,Sun G,Bram RJ,Alugupalli KR

48-0051 was used in Flow cytometry/Cell sorting to examine the role of BAFFR and TACI in T cell-independent antibody responses to an active Borrelia hermsii infection.

Wed Jan 01 00:00:00 EST 2014

Themis sets the signal threshold for positive and negative selection in T-cell development.

Nature

Fu G,Casas J,Rigaud S,Rybakin V,Lambolez F,Brzostek J,Hoerter JA,Paster W,Acuto O,Cheroutre H,Sauer K,Gascoigne NR

48-0051 was used in Flow cytometry/Cell sorting to show that the T-cell-specific protein Themis can specifically attenuate TCR signal strength via SHP1 recruitment and activation in response to low-affinity TCR engagement.

Thu Dec 19 00:00:00 EST 2013

Clonally diverse T cell homeostasis is maintained by a common program of cell-cycle control.

Journal of immunology (Baltimore, Md. : 1950)

Hogan T,Shuvaev A,Commenges D,Yates A,Callard R,Thiebaut R,Seddon B

48-0051 was used in Flow cytometry/Cell sorting to analyse the heterogenous proliferative response of T cell clones to lymphopenia using in vivo and mathematical models.

Mon Apr 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD antigen CD5; CD5; CD5 antigen (p56 62); CD5 antigen p56-62; fCD5; Ly-1; Ly12; LyA; Lymphocyte antigen 1; Pan T cell; T-cell surface glycoprotein CD5

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Gene Aliases: Cd5; Ly-1; Ly-12; Ly-A; Lyt-1

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UniProt ID: (Mouse) P13379

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Entrez Gene ID: (Mouse) 12507

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Function(s)

glycoprotein binding scavenger receptor activity protein binding

Process(es)

T cell costimulation apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD5 Monoclonal Antibody (53-7.3), eFluor™ 450, eBioscience™

CD5 Antibody (48-0051-82) in Flow

Product Details

48-0051-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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