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Invitrogen
CD5 Monoclonal Antibody (UCHT2), eBioscience™
Product Details
14-0059-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
UCHT2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467083
Product Specific Information
Description: The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.
The monoclonal antibody UCHT2 recognizes primate CD5.
Applications Reported: The UCHT2 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: The UCHT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD antigen CD5; CD5; CD5 antigen (p56 62); CD5 antigen (p56-62); CD5 antigen p56-62; fCD5; Ly12; LyA; Lymphocyte antigen T1/Leu-1; Pan T cell; T-cell surface glycoprotein CD5
Gene Aliases: CD5; LEU1; T1
UniProt ID: (Human) P06127
Entrez Gene ID: (Human) 921
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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