Invitrogen

CD5 Monoclonal Antibody (YKIX322.3), PerCP-eFluor™ 710, eBioscience™

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CD5 Antibody (46-5050-42) in Flow

Staining of normal canine peripheral blood cells with Anti-Canine CD8a APC (Product # 17-5080-41) and Rat IgG2a kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or Anti-Canine CD5 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-5050-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog, Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YKIX322.3

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596668

Product Specific Information

Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines.

Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Comparative Immunogenomics of Canine Natural Killer Cells as Immunotherapy Target.

Frontiers in immunology

Gingrich AA,Reiter TE,Judge SJ,York D,Yanagisawa M,Razmara A,Sturgill I,Basmaci UN,Brady RV,Stoffel K,Murphy WJ,Rebhun RB,Brown CT,Canter RJ

46-5050-42 was used in Flow Cytometry to define canine NK cell gene expression under multiple conditions and across species, filling an important gap in translational NK studies.

Wed Sep 27 00:00:00 EDT 2023

Comparative Immunogenomics of Canine Natural Killer Cells as Immunotherapy Target.

Frontiers in immunology

Gingrich AA,Reiter TE,Judge SJ,York D,Yanagisawa M,Razmara A,Sturgill I,Basmaci UN,Brady RV,Stoffel K,Murphy WJ,Rebhun RB,Brown CT,Canter RJ

46-5050-42 was used in Flow Cytometry to define canine NK cell gene expression under multiple conditions and across species, filling an important gap in translational NK studies.

Wed Sep 27 00:00:00 EDT 2023

Genetic re-direction of canine primary T cells for clinical trial use in pet dogs with spontaneous cancer.

STAR protocols

Rotolo A,Atherton MJ,Kasper BT,Haran KP,Mason NJ

Published figure using CD5 monoclonal antibody (Product # 46-5050-42) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Establishing a model system for evaluating CAR T cell therapy using dogs with spontaneous diffuse large B cell lymphoma.

Oncoimmunology

Panjwani MK,Atherton MJ,MaloneyHuss MA,Haran KP,Xiong A,Gupta M,Kulikovsaya I,Lacey SF,Mason NJ

Published figure using CD5 monoclonal antibody (Product # 46-5050-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Clonal relationships of CSF B cells in treatment-naive multiple sclerosis patients.

JCI insight

Eggers EL,Michel BA,Wu H,Wang SZ,Bevan CJ,Abounasr A,Pierson NS,Bischof A,Kazer M,Leitner E,Greenfield AL,Demuth S,Wilson MR,Henry RG,Cree BA,Hauser SL,von Büdingen HC

46-5050-42 was used in Flow Cytometry to find increased CSF lymphocytes with a disproportionate increase of B cells compared with T cells in patients with gadolinium-enhancing (Gd+) lesions on brain MRI.

Thu Nov 16 00:00:00 EST 2017

Bioinformatics

Protein Aliases: CD antigen CD5; CD5 antigen (p56 62); CD5 antigen p56-62; fCD5; Ly12; LyA; Pan T cell

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Entrez Gene ID: (Dog) 612338