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Invitrogen
CD5 Monoclonal Antibody (YKIX322.3), APC-eFluor™ 780, eBioscience™
FIGURE: 1 / 7
CD5 Antibody (47-5050-42) in Flow
Normal canine peripheral blood cells were stained with CD4 Monoclonal Antibody, APC (Product # 17-5040-82) and Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or CD5 Monoclonal Antibody, APC-eFluor 780 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
47-5050-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Published species
Dog
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
YKIX322.3
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784709
Product Specific Information
Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines.
Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YKIX322.3 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Target Information
CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD antigen CD5; CD5 antigen (p56 62); CD5 antigen p56-62; fCD5; Ly12; LyA; Pan T cell
Entrez Gene ID: (Dog) 612338
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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