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Invitrogen
CD51 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
CD51 Antibody (PA5-47096) in ICC/IF
Immunofluorescence analysis of CD51 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD51 Polyclonal Antibody (Product # PA5-47096) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a dilution... View More
Product Details
PA5-47096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human Integrin alpha V/CD51 Phe31-Val992
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609681
Product Specific Information
In Western blots, less than 1% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse Integrin E is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha v integrin; antigen identified by monoclonal antibody L230; CD51; Dnmt3l-ps1 pseudogene; GP3A; integrin alpha V; Integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGB3; Vitronectin receptor; vitronectin receptor alpha polypeptide (VNRA); Vitronectin receptor subunit alpha; VNR
Gene Aliases: 1110004F14Rik; 2610028E01Rik; CD51; D430040G12Rik; ITGAV; MSK8; VNRA; VTNR
UniProt ID: (Human) P06756, (Mouse) P43406
Entrez Gene ID: (Human) 3685, (Rat) 296456, (Mouse) 16410
virus receptor activity
opsonin binding
fibronectin binding
protease binding
protein kinase C binding
voltage-gated calcium channel activity
protein binding
coreceptor activity
fibroblast growth factor binding
C-X3-C chemokine binding
insulin-like growth factor I binding
neuregulin binding
metal ion binding
transforming growth factor beta binding
extracellular matrix binding
extracellular matrix protein binding
receptor binding
peptide binding
protein heterodimerization activity
integrin
angiogenesis
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
positive regulation of cell proliferation
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
cell growth
cell migration
extracellular matrix organization
positive regulation of cell migration
cell-substrate adhesion
negative regulation of lipid transport
positive regulation of osteoblast proliferation
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
apolipoprotein A-I-mediated signaling pathway
apoptotic cell clearance
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of cell adhesion
viral entry into host cell
vascular endothelial growth factor receptor signaling pathway
negative regulation of lipoprotein metabolic process
regulation of phagocytosis
leukocyte migration
negative chemotaxis
entry of symbiont into host cell by promotion of host phagocytosis
ERK1 and ERK2 cascade
calcium ion transmembrane transport
extrinsic apoptotic signaling pathway in absence of ligand
regulation of apoptotic cell clearance
negative regulation of entry of bacterium into host cell
negative regulation of extrinsic apoptotic signaling pathway
blood vessel development
vasculogenesis
cellular calcium ion homeostasis
positive regulation of cytosolic calcium ion concentration
positive regulation of MAPK cascade
inflammatory response
multicellular organism development
fertilization
cell differentiation
detection of molecule of bacterial origin
regulation of ion transmembrane transport
gonad morphogenesis
T cell activation
endothelial cell migration
regulation of bone resorption
focal adhesion assembly
release of sequestered calcium ion into cytosol
trophoblast giant cell differentiation
response to nitric oxide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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