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Product Details
MA5-46830
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
076
Immunogen
Recombinant protein encoding Mouse CD55 (Met1-Thr361).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937902
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD55 (Met1-Thr361) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD55; Complement decay-accelerating factor, GPI-anchored; complement-glycosylphosphatidylinositol; Cromer blood group; DAF-GPI; decay accelerating factor 1; GPI anchor addition signal; RP11-357P18.1
Gene Aliases: Cd55; Cd55a; Daf; Daf-GPI; Daf1; GPI-DAF
UniProt ID: (Mouse) Q61475
Entrez Gene ID: (Mouse) 13136
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