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CD55 Antibody (MA5-47652) in ELISA
ELISA capture of a Standard Curve using CD55 monoclonal antibody (Product # MA5-47652) in human recombinant CD55 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Product Details
MA5-47652
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M033
Immunogen
Clone M033 was generated from a proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
Conjugate
Form
Liquid
Concentration
~0.25 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942633
Product Specific Information
Clone M033 was purified using Protein G chromatography. The antibody. The antibody also detects a "native" human recombinant CD55 protein that includes the extracellular region. The antibody can be used for native western blot, immunoprecipitation, protein and capture ELISA, and immunocytochemistry, as well for detecting CD55 in live, unfixed cells.
Target Information
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD55; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Complement decay-accelerating factor; RP11-357P18.1
Gene Aliases: CD55; CR; CROM; DAF; TC
UniProt ID: (Human) P08174
Entrez Gene ID: (Human) 1604
ER to Golgi vesicle-mediated transport
complement activation, classical pathway
positive regulation of cytosolic calcium ion concentration
regulation of complement activation
regulation of lipopolysaccharide-mediated signaling pathway
CD4-positive, alpha-beta T cell cytokine production
innate immune response
respiratory burst
negative regulation of complement activation
viral entry into host cell
positive regulation of CD4-positive, alpha-beta T cell activation
positive regulation of CD4-positive, alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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