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FIGURE: 1 / 1
CD58 Antibody (PA5-96389) in WB
Western blot analysis of CD58 in extracts of various cell lines. Samples were incubated with CD58 Polyclonal antibody (Product # PA5-96389) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96389
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-220 of human CD58 (NP_0017701)
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808191
Product Specific Information
Immunogen sequence: NVTFHVPSNV PLKEVLWKKQ KDKVAELENS EFRAFSSFKN RVYLDTVSGS LTIYNLTSSD EDEYEMESPN ITDTMKFFLY VLESLPSPTL TCALTNGSIE VQCMIPEHYN SHRGLIMYSW DCPMEQCKRN STSIYFKMEN DLPQKIQCTL SNPLFNTTSS IILTTCIPSS GHSRHRYALI P; Positive Samples: MCF7, 293T; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor, Single-pass type I membrane protein
Target Information
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ag3; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); FLJ23181; FLJ43722; LAIR1; Lymphocyte function-associated antigen 3; RP5-1086K13.2; Surface glycoprotein LFA-3
Gene Aliases: ag3; CD58; LFA-3; LFA3
UniProt ID: (Human) P19256
Entrez Gene ID: (Human) 965
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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