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Invitrogen
CD58 (LFA-3) Monoclonal Antibody (TS2/9), APC, eBioscience™
-Flow-20170216130641.jpg?time=20220826 "CD58 (LFA-3) Antibody in Flow Cytometry (Flow)")
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD58 (LFA-3) Antibody (17-0578-42) in Flow")
Product Details
17-0578-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TS2/9
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10667895
Product Specific Information
Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity.
The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region.
Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TS2/9 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Ag3; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); FLJ23181; FLJ43722; LAIR1; Lymphocyte function-associated antigen 3; RP5-1086K13.2; Surface glycoprotein LFA-3
Gene Aliases: ag3; CD58; LFA-3; LFA3
UniProt ID: (Human) P19256
Entrez Gene ID: (Human) 965
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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