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Product Details
MA1-80430
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1.2B6
Immunogen
Human E-Selectin (ELAM-1).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_929204
Product Specific Information
MA1-80430 inhibits neutrophil adhesion to E-selectin and to P-selectin.
Non-reducing conditions are recommended for Western blot applications. A suggested positive control is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.
Target Information
CD62E represents the 97 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.CD62P is a 140 kDa membrane glycoprotein formerly known as platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. It is expressed on platelets, megakaryocytes, and epithelium. It is thought to mediate the adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses and of activated platelets to neutrophils and monocytes in hemostasis. The monoclonal antibody 1.2B6 recognizes a common epitope shared by CD62E and CD62P.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62 antigen-like family member P; CD62E; CD62P; CD62P; CD62 homolog; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM-2; LECAM-3; LECAM2; LECAM3; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-117P20.2; selectin E (endothelial adhesion molecule 1); selectin P (granule membrane protein 140kDa, antigen CD62)
Gene Aliases: CD62; CD62E; CD62P; ELAM; ELAM1; ESEL; GMP140; GMRP; GRMP; LECAM2; LECAM3; PADGEM; PSEL; SELE; SELP
UniProt ID: (Human) P16581, (Pig) P98110, (Human) P16109
Entrez Gene ID: (Human) 6401, (Pig) 397508, (Pig) 397150, (Human) 6403
transmembrane signaling receptor activity
protein binding
sialic acid binding
phospholipase binding
oligosaccharide binding
carbohydrate binding
metal ion binding
lipopolysaccharide binding
glycoprotein binding
heparin binding
fucose binding
glycosphingolipid binding
calcium-dependent protein binding
platelet degranulation
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
response to lipopolysaccharide
regulation of integrin activation
calcium-mediated signaling using intracellular calcium source
positive regulation of cell adhesion
defense response to Gram-negative bacterium
leukocyte migration
leukocyte tethering or rolling
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
activation of phospholipase C activity
calcium-mediated signaling
actin filament-based process
response to tumor necrosis factor
regulation of inflammatory response
response to interleukin-1
positive regulation of leukocyte tethering or rolling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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