Invitrogen

CD63 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

9 References

View all (139) CD63 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD63 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

CD63 Antibody (PA5-92370) in ICC/IF

Immunofluorescence analysis of CD63 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD63 Polyclonal Antibody (Product # PA5-92370) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More

CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CD63 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of the target protein. U-87 MG cells were transfected with CD63 siRNA and a decrease in signal intensity was observed in ICC application using Anti-CD63 Polyclonal Antibody (Product # PA5-92370). {KD}

Product Details

PA5-92370

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:100

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_0017711)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806456

Product Specific Information

Immunogen sequence: AGYVFRDKVM SEFNNNFRQQ MENYPKNNHT ASILDRMQAD FKCCGAANYT DWEKIPSMSK NRVPDSCCIN VTVGCGINFN EKAIHKEGCV EKIGGWLRKN V; Positive Samples: A375, 22Rv1, Mouse kidney, Mouse spleen; Cellular Location: Cell membrane, Endosome, Late endosome membrane, Lysosome membrane, Melanosome, Multi-pass membrane protein, multivesicular body

Target Information

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bone marrow stromal cell-derived exosomes improve oxidative stress and pyroptosis in doxorubicin-induced myocardial injury in vitro by regulating the transcription of GSDMD through the PI3K-AKT-Foxo1 pathway.

Immunity, inflammation and disease

Zeng H,Yang Y,Tou F,Zhan Y,Liu S,Zou P,Chen Y,Shao L

Published figure using CD63 polyclonal antibody (Product # PA5-92370) in Western Blot

Wed Mar 01 00:00:00 EST 2023

MicroRNA Cargo in Wharton's Jelly MSC Small Extracellular Vesicles: Key Functionality to In Vitro Prevention and Treatment of Premature White Matter Injury.

Stem cell reviews and reports

Tscherrig V,Cottagnoud S,Haesler V,Renz P,Surbek D,Schoeberlein A,Joerger-Messerli MS

Published figure using CD63 polyclonal antibody (Product # PA5-92370) in Western Blot

Sun Oct 01 00:00:00 EDT 2023

Muscle-derived stem cell exosomes with overexpressed miR-214 promote the regeneration and repair of rat sciatic nerve after crush injury to activate the JAK2/STAT3 pathway by targeting PTEN.

Frontiers in molecular neuroscience

Zeng X,Bian W,Liu Z,Li J,Ren S,Zhang J,Zhang H,Tegeleqi B,He G,Guan M,Gao Z,Huang C,Liu J

PA5-92370 was used in Western Blot to investigate the effect of muscle-derived stem cell (MDSC) exosomes with overexpressed miR-214 on the regeneration and repair of rat sciatic nerve after crush injury and its molecular mechanism.

Tue Jun 13 00:00:00 EDT 2023

Muscle-derived stem cell exosomes with overexpressed miR-214 promote the regeneration and repair of rat sciatic nerve after crush injury to activate the JAK2/STAT3 pathway by targeting PTEN.

Frontiers in molecular neuroscience

Zeng X,Bian W,Liu Z,Li J,Ren S,Zhang J,Zhang H,Tegeleqi B,He G,Guan M,Gao Z,Huang C,Liu J

Tue Jun 13 00:00:00 EDT 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

PA5-92370 was used in Western Blot to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 EST 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

Wed Mar 01 00:00:00 EST 2023

Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats.

Neural regeneration research

Li C,Liu SY,Zhang M,Pi W,Wang B,Li QC,Lu CF,Zhang PX

Published figure using CD63 polyclonal antibody (Product # PA5-92370) in Western Blot

Thu Sep 01 00:00:00 EDT 2022

Neural stem cell-derived exosomes suppress neuronal cell apoptosis by activating autophagy via miR-374-5p/STK-4 axis in spinal cord injury.

Journal of musculoskeletal & neuronal interactions

Zhang L,Han P

PA5-92370 was used in Western Blot to evaluate the roles of MicroRNAs (miRNAs) enclosed in the neuron-derived exosomes in the recovery of the spinal cord injury (SCI) and the mechanism.

Thu Sep 01 00:00:00 EDT 2022

Neural stem cell-derived exosomes suppress neuronal cell apoptosis by activating autophagy via miR-374-5p/STK-4 axis in spinal cord injury.

Journal of musculoskeletal & neuronal interactions

Zhang L,Han P

PA5-92370 was used in Western Blot to evaluate the roles of MicroRNAs (miRNAs) enclosed in the neuron-derived exosomes in the recovery of the spinal cord injury (SCI) and the mechanism.

Thu Sep 01 00:00:00 EDT 2022

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

Laso-García F,Piniella D,Gómez-de Frutos MC,Casado-Fernández L,Pérez-Mato M,Alonso-López E,Otero-Ortega L,Bravo SB,Chantada-Vázquez MDP,Trilla-Fuertes L,Fresno-Vara JÁ,Fuentes B,Díez-Tejedor E,Gutiérrez-Fernández M,Alonso De Leciñana M

PA5-92370 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to describe the number and protein content of blood-derived EVs following an intracerebral hemorrhage (ICH),.

Tue Feb 14 00:00:00 EST 2023

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

PA5-92370 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to describe the number and protein content of blood-derived EVs following an intracerebral hemorrhage (ICH),.

Tue Feb 14 00:00:00 EST 2023

A Novel Localization in Human Large Extracellular Vesicles for the EGF-CFC Founder Member CRIPTO and Its Biological and Therapeutic Implications.

Cancers

Mantile F,Kisovec M,Adamo G,Romancino DP,Hočevar M,Božič D,Bedina Zavec A,Podobnik M,Stoppelli MP,Kisslinger A,Bongiovanni A,Kralj-Iglič V,Liguori GL

PA5-92370 was used in Western Blotting to investigate the effect of EVs released by teratocarcinoma cells on glioblastoma (GBM) cells.

Fri Jul 29 00:00:00 EDT 2022

Macrophage secretion of miR-106b-5p causes renin-dependent hypertension.

Nature communications

Oh J,Matkovich SJ,Riek AE,Bindom SM,Shao JS,Head RD,Barve RA,Sands MS,Carmeliet G,Osei-Owusu P,Knutsen RH,Zhang H,Blumer KJ,Nichols CG,Mecham RP,Baldán Á,Benitez BA,Sequeira-Lopez ML,Gomez RA,Bernal-Mizrachi C

PA5-92370 was used in Western Blotting to confirm that macrophage miR-106b-5p secretion from impaired vitamin D receptor signalling causes inflammation-induced hypertension.

Wed Sep 23 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

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Gene Aliases: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

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UniProt ID: (Human) P08962

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Entrez Gene ID: (Human) 967

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Function(s)

protein binding protein complex binding

Process(es)

positive regulation of receptor internalization platelet degranulation cell-matrix adhesion negative regulation of epithelial cell migration protein transport cell migration epithelial cell differentiation cellular protein localization endosome to melanosome transport positive regulation of cell adhesion pigment granule maturation regulation of vascular endothelial growth factor signaling pathway regulation of rubidium ion transport positive regulation of integrin-mediated signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD63 Polyclonal Antibody

CD63 Antibody (PA5-92370) in ICC/IF

Product Details

PA5-92370

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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