Invitrogen

CD64 Monoclonal Antibody (10.1)

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CD64 Antibody (MA1-10270) in Flow

Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD64 (10.1) purified Monoclonal antibody (Product # MA1-10270) (concentration in sample 4 µg/mL) GAM APC.

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Product Details

MA1-10270

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

1 - 5 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

10.1

Immunogen

Rheumatoid synovial fluid cells and fibronectin purified human monocytes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152864

Product Specific Information

This antibody recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to formalin fixation.

Target Information

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System.

Journal of immunology (Baltimore, Md. : 1950)

Waschbisch A,Schröder S,Schraudner D,Sammet L,Weksler B,Melms A,Pfeifenbring S,Stadelmann C,Schwab S,Linker RA

MA110270 was used in flow cytometry to analyze the frequency and phenotype of monocyte subpopulations in peripheral blood, cerebrospinal fluid, and brain biopsy material derived from multiple sclerosis patients

Mon Feb 15 00:00:00 EST 2016

Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.

Human immunology

Garbe K,Bratke K,Wagner S,Virchow JC,Lommatzsch M

MA1-10270 was used in flow cytometry to examine the effects of age and gender on TLR9 expression on human DCs.

Tue May 01 00:00:00 EDT 2012

Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake.

The Journal of experimental medicine

Willcocks LC,Lyons PA,Clatworthy MR,Robinson JI,Yang W,Newland SA,Plagnol V,McGovern NN,Condliffe AM,Chilvers ER,Adu D,Jolly EC,Watts R,Lau YL,Morgan AW,Nash G,Smith KG

MA1-10270 was used in flow cytometry to examine how FCGR3B copy number affects immune responses

Mon Jul 07 00:00:00 EDT 2008

Engagement of CD83 ligand induces prolonged expansion of CD8+ T cells and preferential enrichment for antigen specificity.

Blood

Hirano N,Butler MO,Xia Z,Ansén S,von Bergwelt-Baildon MS,Neuberg D,Freeman GJ,Nadler LM

MA1-10270 was used in flow cytometry to examine the consequences of CD83 ligand engagement on antigen-specific CD8+ T cells

Wed Feb 15 00:00:00 EST 2006

Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).

Journal of immunological methods

Contamin H,Loizon S,Bourreau E,Michel JC,Garraud O,Mercereau-Puijalon O,Behr C

MA1-10270 was used in flow cytometry to discuss available reagents for studying the neotropical primate squirrel monkey.

Tue Feb 01 00:00:00 EST 2005

Prognostic significance of CD56 antigen expression in acute myeloid leukemia.

Haematologica

Di Bona E,Sartori R,Zambello R,Guercini N,Madeo D,Rodeghiero F

MA1-10270 was used in flow cytometry to examine CD56 expression in a cohort of acute myeloid leukemia patients in to assess its frequency and prognostic relevance.

Fri Mar 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: CD64; CD64 antigen; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; RP11-196G18.2

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Gene Aliases: CD64; CD64A; FCG1; FCGR1; FCGR1A; FCRI; IGFR1

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UniProt ID: (Human) P12314

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Entrez Gene ID: (Human) 2209

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin receptor superfamily

Process(es)

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent receptor-mediated endocytosis phagocytosis, engulfment immune response signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD64 Monoclonal Antibody (10.1)

CD64 Antibody (MA1-10270) in Flow

Product Details

MA1-10270

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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