Invitrogen

CD64 Monoclonal Antibody (X54-5/7.1), APC, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

4 References

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FIGURE: 1 / 3

CD64 Antibody (17-0641-82) in Flow

BALB/c mouse bone marrow cells were Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or 0.25 µg of CD64 Monoclonal Antibody, APC (right). All cells were used for analysis.

Product Image Gallery

CD64 Antibody — Staining of mouse bone marrow. As expected based on the known relative expression patterns, CD64 clone X54-5/7.1 stains monocytes with the highest intensity (purple histogram) and B cell precursors with lower intensity (orange and blue histograms). Details: Mouse bone marrow was Fc blocked, stained with CD64 (clone X54-5/7.1), and co-stained with CD45R (B220) (clone RA3-6B2) and CD43 (clone eBioR2/60). Cells in the indicated color gates were used for analysis. {RE}

Product Details

17-0641-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

X54-5/7.1

Immunogen

BALB/c mouse CD64-human IgG Fc fusion protein

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735010

Product Specific Information

Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line.

Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Science advances

Liu J,Lane S,Lall R,Russo M,Farrell L,Debreli Coskun M,Curtin C,Araujo-Gutierrez R,Scherrer-Crosbie M,Trachtenberg BH,Kim J,Tolosano E,Ghigo A,Gerszten RE,Asnani A

Published figure using CD64 monoclonal antibody (Product # 17-0641-82) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2022

Protective role of cortistatin in pulmonary inflammation and fibrosis.

British journal of pharmacology

Barriga M,Benitez R,Ferraz-de-Paula V,Garcia-Frutos M,Caro M,Robledo G,O'Valle F,Campos-Salinas J,Delgado M

17-0641-82 was used in Flow Cytometry to conclude that cortistatin-based therapies could emerge as attractive candidates to treat severe ALI/ARDS, including SARS-CoV-2-associated ARDS.

Mon Nov 01 00:00:00 EDT 2021

The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching.

Immunity

Xu J,Zanvit P,Hu L,Tseng PY,Liu N,Wang F,Liu O,Zhang D,Jin W,Guo N,Han Y,Yin J,Cain A,Hoon MA,Wang S,Chen W

17-0641 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying itching associated with cutaneous wound healing, focusing on the contribution of soluble factors released during healing.

Tue Aug 18 00:00:00 EDT 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

17-0641-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

Bioinformatics

Protein Aliases: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2

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Gene Aliases: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC

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UniProt ID: (Mouse) P26151

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Entrez Gene ID: (Mouse) 14129

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Function(s)

IgG receptor activity IgG binding leukotriene receptor binding immunoglobulin receptor superfamily

Process(es)

antibody-dependent cellular cytotoxicity positive regulation of type IIa hypersensitivity positive regulation of type III hypersensitivity immune system process receptor-mediated endocytosis phagocytosis, recognition phagocytosis, engulfment cell surface receptor signaling pathway response to bacterium antigen processing and presentation of exogenous antigen Fc-gamma receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium innate immune response positive regulation of phagocytosis regulation of immune response positive regulation of phagocytosis, engulfment

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CD64 Monoclonal Antibody (X54-5/7.1), APC, eBioscience™

CD64 Antibody (17-0641-82) in Flow

Product Details

17-0641-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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