Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
CD65 Antibody (MA5-44114) in Flow
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD65 (VIM8) PE Monoclonal antibody (Product # MA5-44114) using a dilution of 10 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-44114
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
VIM8
Immunogen
THP-1 cell line
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913046
Product Specific Information
This antibody recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
Target Information
This type 2 fucosylated carbohydrate antigen is expressed on the surface of all myeloid cells during development. Moreover, CD65 is expressed highly on granulocytes and weakly on monocytes in peripheral blood. CD65 has been reported to be a ligand for CD62E (E-Selectin). However, the sialylated form of CD65 (designated CD65s) can be used as a marker for acute myeloid leukemia (AML) as its expression increases as CD34 decreases. Finally, CD65 is important for extravascular infiltration of AML cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Fucoganglioside Type 2; Fucoganglioside Type II
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support