Invitrogen

CD66 Monoclonal Antibody (CD66a-B1.1), Super Bright™ 702, eBioscience™

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View all (43) CD66 (CEACAM) antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

67-0668-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 702, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD66a-B1.1

Conjugate

Super Bright™ 702 Super Bright™ 702 Super Bright™ 702

Excitation/Emission Max

413/702 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762578

Product Specific Information

Description: The CD66a-B1.1 monoclonal antibody reacts with human CD66a, c, d, and e, which are also known as CEACAM1, CEACAM6, CEACAM3 and CEA, respectively. CD66 is expressed on endothelial and epithelial cells, as well as on neutrophils and monocytes. Myeloma and cancer cells also express CD66. Moreover, expression of this receptor can be induced on T cells, B cells, and CD16-negative NK cells. CD66 exists as multiple splice variants that differ in the length of their cytoplasmic domain, which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that mediates the inhibitory functions of this cell surface protein on T and NK cells. CD66 is involved in cell adhesion and growth, neutrophil activation, and signaling. Finally, studies have shown that the CD66 variants interact with each other homo- and heterotypically.

Applications Reported: This CD66a-B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD66a-B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: antigen CD66; BGP-1; Biliary glycoprotein 1; Carcinoembryonic antigen; Carcinoembryonic antigen CGM1; Carcinoembryonic antigen CGM7; carcinoembryonic antigen gene family member 1; carcinoembryonic antigen gene family member 7; Carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); Carcinoembryonic antigen-related cell adhesion molecule 3; Carcinoembryonic antigen-related cell adhesion molecule 4; carcinoembryonic antigen-related cell adhesion molecule 4-sv1; carcinoembryonic antigen-related cell adhesion molecule 4-sv2; Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 6; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); CD66a; CD66a antigen; CD66c; CD66d; CD66d antigen; CD66e; CEA; CEA cell adhesion molecule 4; CEACAM-1; CEACAM-3; CEACAM-5; CEACAM-6; Cluster of Differentiation 66c; Meconium antigen 100; Non-specific cross-reacting antigen W236; Non-specific crossreacting antigen; nonspecific cross-reacting antigen; Nonspecific cross-reacting antigen (NCA); nonspecific cross-reacting antigen W236; Normal cross-reacting antigen; pan CEACAM

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Gene Aliases: BGP; BGP1; BGPI; CD66c; CD66D; CD66e; CEA; CEACAM1; CEACAM3; CEACAM4; CEACAM5; CEACAM6; CEAL; CGM1; CGM7; CGM7_HUMAN; NCA; W264; W282

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UniProt ID: (Human) A6NE38, (Human) P40198, (Human) O75871, (Human) P06731, (Human) P40199

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Entrez Gene ID: (Human) 634, (Human) 1084, (Human) 1089, (Human) 1048, (Human) 4680

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Function(s)
molecular_function actin binding protein binding bile acid transmembrane transporter activity kinase binding protein phosphatase binding filamin binding protein homodimerization activity protein dimerization activity protein tyrosine kinase binding GPI anchor binding identical protein binding
Process(es)
phagocytosis angiogenesis regulation of cell growth blood vessel development negative regulation of T cell mediated cytotoxicity negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of protein kinase activity cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules integrin-mediated signaling pathway regulation of endothelial cell migration regulation of phosphatidylinositol 3-kinase signaling bile acid and bile salt transport cell migration regulation of cell migration negative regulation of granulocyte differentiation negative regulation of interleukin-1 production cellular response to insulin stimulus common myeloid progenitor cell proliferation insulin receptor internalization granulocyte colony-stimulating factor signaling pathway regulation of epidermal growth factor receptor signaling pathway negative regulation of vascular permeability negative regulation of cytotoxic T cell degranulation wound healing, spreading of cells regulation of endothelial cell differentiation negative regulation of fatty acid biosynthetic process negative regulation of T cell receptor signaling pathway leukocyte migration negative regulation of lipid biosynthetic process regulation of blood vessel remodeling regulation of ERK1 and ERK2 cascade negative regulation of platelet aggregation cell-cell adhesion insulin catabolic process regulation of homophilic cell adhesion regulation of sprouting angiogenesis negative regulation of hepatocyte proliferation positive regulation of vasculogenesis signal transduction cell-cell signaling negative regulation of myotube differentiation homotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of anoikis
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