Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD68 Monoclonal Antibody (514H12)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CD68 Antibody (MA1-80133) in ICC/IF
Immunofluorescence analysis of CD68 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0 phase). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD68 Mouse Monoclonal Antibody (514H12) (Product # MA1-80133) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibo... View More
Product Details
MA1-80133
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
514H12
Immunogen
Fusion protein of external domain of human CD68.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_929283
Product Specific Information
Heat-mediated antigen retrieval using Tris/EDTA (pH 8) is recommended for the staining of paraffin sections. The epitope recognized by clone 514H12 is sensitive to peroxide and so any peroxidase-blocking steps should be performed after incubation with this antibody. A suggested positive control for immunohistochemical applications is human tonsil.
Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a approximately 110 kDa glycoprotein primarily expressed by macrophages and monocytes.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1
Gene Aliases: CD68; GP110; LAMP4; SCARD1
UniProt ID: (Human) P34810
Entrez Gene ID: (Human) 968
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support