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Invitrogen
CD68 Monoclonal Antibody (FA-11), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 9
CD68 Antibody (53-0681-82) in Flow
Mouse bone marrow-derived macrophages were surface stained with Mouse CD11b Monoclonal Antibody, eFluor 450 (Product # 48-0112-82) followed by intracellular staining with 0.125 µg of Rat IgG2a K Isotype Control Antibody, Alexa Fluor 488 (Product # 53-4321-80) (left) or 0.125 µg of CD68 Monoclonal Antibody, Alexa Fluor 488 (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
53-0681-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FA-11
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784745
Product Specific Information
Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin. CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP). Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells. CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. In the steady state, only about 10-15% of CD68 is present at the surface. Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling. CD68 binds oxidized LDL and is involved in the process of phagocytosis.
The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues.
Applications Reported: This FA-11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This FA-11 antibody has been tested by flow cytometric analysis of mouse bone marrow marcophages. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD68; Macrosialin
Gene Aliases: Cd68; gp110; Lamp4; Scard1
UniProt ID: (Mouse) P31996
Entrez Gene ID: (Mouse) 12514
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