Invitrogen

CD68 Monoclonal Antibody (FA-11)

CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD68 Antibody (MA5-16674) in IHC (F)

Immunohistochemical staining of mouse spleen cryosection with CD68/gp110 Antibody (Product # MA5-16674) followed by Goat anti Rat IgG, HRP conjugated secondary showing Immunohistochemical staining of macrophages in the red pulp

CD68 Antibody in Immunohistochemistry (IHC)

CD68 Antibody in Immunohistochemistry, Immunohistochemistry - Free Floating (IHC, IHC (Free))

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Product Details

MA5-16674

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

FA-11

Immunogen

purified Con A acceptor glycoprotein from P815 cell line

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4°C or -20°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538168

Product Specific Information

Non-reducing conditions are recommended for Western blot applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysis.

PeerJ

Li JL,Fu GQ,Wang YY,Bian MM,Xu YM,Zhang L,Chen YQ,Zhang N,Ding SQ,Wang R,Fang R,Tang J,Hu JG,Lü HZ

MA5-16674 was used in Immunohistochemistry-immunofluorescence to study if M1 cells in injured spinal cords were mainly derived from MG rather than infiltrating Mø.

Tue Feb 28 00:00:00 EST 2023

Exploring the Effect and Mechanism of Si-Miao-Yong-An Decoction on Abdominal Aortic Aneurysm Based on Mice Experiment and Bioinformatics Analysis.

Evidence-based complementary and alternative medicine : eCAM

Xu Z,Zhang L,Huangfu N,Jiang F,Ji K,Wang S

Published figure using CD68 monoclonal antibody (Product # MA5-16674) in Immunohistochemistry

Sat Jul 16 00:00:00 EDT 2022

A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach.

Cellular and molecular gastroenterology and hepatology

Douchi D,Yamamura A,Matsuo J,Lee JW,Nuttonmanit N,Melissa Lim YH,Suda K,Shimura M,Chen S,Pang S,Kohu K,Kaneko M,Kiyonari H,Kaneda A,Yoshida H,Taniuchi I,Osato M,Yang H,Unno M,Bok-Yan So J,Yeoh KG,Chuang LSH,Bae SC,Ito Y

Published figure using CD68 monoclonal antibody (Product # MA5-16674) in Immunohistochemistry

Mon Apr 18 00:00:00 EDT 2022

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

MA5-16674 was used in Immunohistochemistry to suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18.

Wed Sep 01 00:00:00 EDT 2021

H3.3 K36M Mutation as a Clinical Diagnosis Method of Suspected Chondroblastoma Cases.

Orthopaedic surgery

Mu H,Jiang Y,Xue L,Hua Y,Lin J,Cai Z

MA5-16674 was used in Immunohistochemistry to distinguish suspected CB from other giant cell-rich or cartilage matrix-diffuse bone tumors.

Thu Apr 01 00:00:00 EDT 2021

Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β.

The Journal of biological chemistry

Nies SH,Takahashi H,Herber CS,Huttner A,Chase A,Strittmatter SM

MA5-16674 was used in Immunohistochemistry to assesses the impact of Aβ copathology, of deleting loci known to modify Alzheimer's disease risk (Ptk2b, Grn, and Tmem106b) and of pharmacological intervention with an Fyn kinase inhibitor on tau spreading after injection of AD tau extracts.

Fri Oct 01 00:00:00 EDT 2021

Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β.

The Journal of biological chemistry

Nies SH,Takahashi H,Herber CS,Huttner A,Chase A,Strittmatter SM

Fri Oct 01 00:00:00 EDT 2021

Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β.

The Journal of biological chemistry

Nies SH,Takahashi H,Herber CS,Huttner A,Chase A,Strittmatter SM

Fri Oct 01 00:00:00 EDT 2021

Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm.

Theranostics

Toczek J,Boodagh P,Sanzida N,Ghim M,Salarian M,Gona K,Kukreja G,Rajendran S,Wei L,Han J,Zhang J,Jung JJ,Graham M,Liu X,Sadeghi MM

MA5-16674 was used in Immunohistochemistry to investigate the feasibility of X-ray computed tomography (CT) imaging of phagocytic activity using nanoparticle contrast agents to predict AAA outcome.

Fri Jul 30 00:00:00 EDT 2021

CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironment.

Journal of neuroinflammation

Chen YQ,Wang SN,Shi YJ,Chen J,Ding SQ,Tang J,Shen L,Wang R,Ding H,Hu JG,Lü HZ

MA5-16674 was used in Immunohistochemistry (Frozen) to study the effect of CRID3 on the local microenvironment and the possible role in neuroprotection following spinal cord injury.

Sat Aug 29 00:00:00 EDT 2020

Lymphangiogenic therapy prevents cardiac dysfunction by ameliorating inflammation and hypertension.

eLife

Song L,Chen X,Swanson TA,LaViolette B,Pang J,Cunio T,Nagle MW,Asano S,Hales K,Shipstone A,Sobon H,Al-Harthy SD,Ahn Y,Kreuser S,Robertson A,Ritenour C,Voigt F,Boucher M,Sun F,Sessa WC,Roth Flach RJ

MA5-16674 was used in Immunohistochemistry to demonstrate that VEGFCc156s had a multifaceted therapeutic effect to prevent angiotensin II-induced cardiac dysfunction by improving cardiac lymphatic function, alleviating fibrosis and inflammation, and ameliorating hypertension.

Tue Nov 17 00:00:00 EST 2020

Myeloperoxidase inhibition in mice alters atherosclerotic lesion composition.

PloS one

Roth Flach RJ,Su C,Bollinger E,Cortes C,Robertson AW,Opsahl AC,Coskran TM,Maresca KP,Keliher EJ,Yates PD,Kim AM,Kalgutkar AS,Buckbinder L

Published figure using CD68 monoclonal antibody (Product # MA5-16674) in Immunohistochemistry

Mon Dec 09 00:00:00 EST 2019

Disease-modifying bioactivity of intravenous cardiosphere-derived cells and exosomes in mdx mice.

JCI insight

Rogers RG,Fournier M,Sanchez L,Ibrahim AG,Aminzadeh MA,Lewis MI,Marbán E

MA5-16674 was used in Immunohistochemistry to demonstrate regenerative responses of cardiac and skeletal muscle tissue to intravenous cardiosphere-derived cells and exosomes in mdx mice.

Thu Apr 04 00:00:00 EDT 2019

Interaction between Plasmodium Glycosylphosphatidylinositol and the Host Protein Moesin Has No Implication in Malaria Pathology.

Frontiers in cellular and infection microbiology

Dunst J,Azzouz N,Liu X,Tsukita S,Seeberger PH,Kamena F

MA5-16674 was used in Flow Cytometry to identify the host protein, moesin, as an interaction partner of Plasmodium glycosylphosphatidylinositol in vitro.

Mon Jan 15 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CD68; Macrosialin

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Gene Aliases: Cd68; gp110; Lamp4; Scard1

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UniProt ID: (Mouse) P31996

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Entrez Gene ID: (Mouse) 12514

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Function(s)

membrane trafficking regulatory protein

Process(es)

cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD68 Monoclonal Antibody (FA-11)

CD68 Antibody (MA5-16674) in IHC (F)

Product Details

MA5-16674

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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