Invitrogen

CD69 Monoclonal Antibody (FN50), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 21

CD69 Antibody (46-0699-42) in Flow

Staining of overnight PHA-stimulated normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0037-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD69 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

46-0699-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FN50

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573694

Product Specific Information

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

An extended SARS-CoV-2 mRNA vaccine prime-boost interval enhances B cell immunity with limited impact on T cells.

iScience

Nicolas A,Sannier G,Dubé M,Nayrac M,Tauzin A,Painter MM,Goel RR,Laporte M,Gendron-Lepage G,Medjahed H,Williams JC,Brassard N,Niessl J,Gokool L,Morrisseau C,Arlotto P,Tremblay C,Martel-Laferrière V,Finzi A,Greenplate AR,Wherry EJ,Kaufmann DE

46-0699-42 was used in flow cytometry to compare SARS-CoV-2 naive donors B and T cell responses to two mRNA vaccine doses administered 3-4 versus 16 weeks apart.

Fri Jan 20 00:00:00 EST 2023

A third SARS-CoV-2 mRNA vaccine dose in people receiving hemodialysis overcomes B cell defects but elicits a skewed CD4⁺ T cell profile.

Cell reports. Medicine

Sannier G,Nicolas A,Dubé M,Marchitto L,Nayrac M,Tastet O,Chatterjee D,Tauzin A,Lima-Barbosa R,Laporte M,Cloutier R,Sreng Flores AM,Boutin M,Gong SY,Benlarbi M,Ding S,Bourassa C,Gendron-Lepage G,Medjahed H,Goyette G,Brassard N,Delgado GG,Niessl J,Gokool L,Morrisseau C,Arlotto P,Rios N,Tremblay C,Martel-Laferrière V,Prat A,Bélair J,Beaubien-Souligny W,Goupil R,Nadeau-Fredette AC,Lamarche C,Finzi A,Suri RS,Kaufmann DE

46-0699-42 was used in Flow cytometry/Cell sorting to show that a third vaccine dose is critical to achieving robust multifaceted immunity in hemodialysis patients, although some distinct TH characteristics endure.

Tue Mar 21 00:00:00 EDT 2023

SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans.

Cell

Mudd PA,Minervina AA,Pogorelyy MV,Turner JS,Kim W,Kalaidina E,Petersen J,Schmitz AJ,Lei T,Haile A,Kirk AM,Mettelman RC,Crawford JC,Nguyen THO,Rowntree LC,Rosati E,Richards KA,Sant AJ,Klebert MK,Suessen T,Middleton WD,Wolf J,Teefey SA,O'Halloran JA,Presti RM,Kedzierska K,Rossjohn J,Thomas PG,Ellebedy AH

46-0699-42 was used in Flow Cytometry to evaluate the T cell receptor sequences and phenotype of SARS-CoV-2 vaccine-induced lymph node follicular helper CD4+ T cells.

Thu Feb 17 00:00:00 EST 2022

SARS CoV-2 mRNA vaccination exposes latent HIV to Nef-specific CD8⁺ T-cells.

Nature communications

Stevenson EM,Terry S,Copertino D,Leyre L,Danesh A,Weiler J,Ward AR,Khadka P,McNeil E,Bernard K,Miller IG,Ellsworth GB,Johnston CD,Finkelsztein EJ,Zumbo P,Betel D,Dündar F,Duncan MC,Lapointe HR,Speckmaier S,Moran-Garcia N,Papa MP,Nicholes S,Stover CJ,Lynch RM,Caskey M,Gaebler C,Chun TW,Bosque A,Wilkin TJ,Lee GQ,Brumme ZL,Jones RB

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen.

Cell reports

Nayrac M,Dubé M,Sannier G,Nicolas A,Marchitto L,Tastet O,Tauzin A,Brassard N,Lima-Barbosa R,Beaudoin-Bussières G,Vézina D,Gong SY,Benlarbi M,Gasser R,Laumaea A,Prévost J,Bourassa C,Gendron-Lepage G,Medjahed H,Goyette G,Ortega-Delgado GG,Laporte M,Niessl J,Gokool L,Morrisseau C,Arlotto P,Richard J,Bélair J,Prat A,Tremblay C,Martel-Laferrière V,Finzi A,Kaufmann DE

46-0699-42 was used in Flow cytometry/Cell sorting to characterized the molecular and behavioral changes in aged 14-3-3γ heterozygous mice to investigate the role of 14-3-3γ in the brain.

Tue Jun 28 00:00:00 EDT 2022

A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responses.

Cell host & microbe

Tauzin A,Nayrac M,Benlarbi M,Gong SY,Gasser R,Beaudoin-Bussières G,Brassard N,Laumaea A,Vézina D,Prévost J,Anand SP,Bourassa C,Gendron-Lepage G,Medjahed H,Goyette G,Niessl J,Tastet O,Gokool L,Morrisseau C,Arlotto P,Stamatatos L,McGuire AT,Larochelle C,Uchil P,Lu M,Mothes W,De Serres G,Moreira S,Roger M,Richard J,Martel-Laferrière V,Duerr R,Tremblay C,Kaufmann DE,Finzi A

46-0699-42 was used in Flow Cytometry to highlight the potential role of Fc-mediated effector functions and T cell responses in vaccine efficacy.

Wed Jul 14 00:00:00 EDT 2021

Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhou C,Wei W,Ma J,Yang Y,Liang L,Zhang Y,Wang Z,Chen X,Huang L,Wang W,Wu S

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Wed Apr 07 00:00:00 EDT 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screen.

PloS one

Liu H,Hu PW,Dubrulle J,Stossi F,Nikolai BC,Mancini MA,Rice AP

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Tue Sep 14 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

Coagulation factor V is a marker of tumor-infiltrating immune cells in breast cancer.

Oncoimmunology

Tinholt M,Stavik B,Tekpli X,Garred Ø,Borgen E,Kristensen V,Sahlberg KK,Sandset PM,Iversen N

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Unique natural killer cell subpopulations are associated with exacerbation risk in chronic obstructive pulmonary disease.

Scientific reports

Osterburg AR,Lach L,Panos RJ,Borchers MT

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon Jan 27 00:00:00 EST 2020

Unique natural killer cell subpopulations are associated with exacerbation risk in chronic obstructive pulmonary disease.

Scientific reports

Osterburg AR,Lach L,Panos RJ,Borchers MT

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon Jan 27 00:00:00 EST 2020

Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.

eLife

Gonzalo-Gil E,Rapuano PB,Ikediobi U,Leibowitz R,Mehta S,Coskun AK,Porterfield JZ,Lampkin TD,Marconi VC,Rimland D,Walker BD,Deeks S,Sutton RE

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Tue Apr 09 00:00:00 EDT 2019

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase.

Chinese medicine

Houng JY,Tai TS,Hsu SC,Hsu HF,Hwang TS,Lin CJ,Fang LW

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Scientific reports

Albert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells.

eLife

Reeder JE,Kwak YT,McNamara RP,Forst CV,D'Orso I

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Thu Apr 10 00:00:00 EDT 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD69 monoclonal antibody (Product # 46-0699-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

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Gene Aliases: AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3

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UniProt ID: (Human) Q07108

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Entrez Gene ID: (Human) 969

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Function(s)

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding

Process(es)

signal transduction cellular response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD69 Monoclonal Antibody (FN50), PerCP-eFluor™ 710, eBioscience™

CD69 Antibody (46-0699-42) in Flow

Product Details

46-0699-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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