Invitrogen

CD69 Monoclonal Antibody (FN50), eFluor™ 450, eBioscience™

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CD69 Antibody (48-0699-42) in Flow

Staining of PHA stimulated normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD69 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

CD69 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

Product Details

48-0699-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FN50

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574025

Product Specific Information

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of PHA stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

SARS CoV-2 mRNA vaccination exposes latent HIV to Nef-specific CD8⁺ T-cells.

Nature communications

Stevenson EM,Terry S,Copertino D,Leyre L,Danesh A,Weiler J,Ward AR,Khadka P,McNeil E,Bernard K,Miller IG,Ellsworth GB,Johnston CD,Finkelsztein EJ,Zumbo P,Betel D,Dündar F,Duncan MC,Lapointe HR,Speckmaier S,Moran-Garcia N,Papa MP,Nicholes S,Stover CJ,Lynch RM,Caskey M,Gaebler C,Chun TW,Bosque A,Wilkin TJ,Lee GQ,Brumme ZL,Jones RB

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhou C,Wei W,Ma J,Yang Y,Liang L,Zhang Y,Wang Z,Chen X,Huang L,Wang W,Wu S

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Wed Apr 07 00:00:00 EDT 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screen.

PloS one

Liu H,Hu PW,Dubrulle J,Stossi F,Nikolai BC,Mancini MA,Rice AP

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Tue Sep 14 00:00:00 EDT 2021

Distinct populations of antigen-specific tissue-resident CD8+ T cells in human cervix mucosa.

JCI insight

Peng T,Phasouk K,Bossard E,Klock A,Jin L,Laing KJ,Johnston C,Williams NA,Czartoski JL,Varon D,Long AN,Bielas JH,Snyder TM,Robins H,Koelle DM,McElrath MJ,Wald A,Corey L,Zhu J

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Immunocytochemistry

Mon Aug 09 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

48-0699-42 was used in Flow Cytometry to demonstrate that due to 17q gain, BIRC5/survivin expression is upregulated in resistant MCL tumor cells and targeting BIRC5 results in marked tumor inhibition in preclinical models.

Mon May 17 00:00:00 EDT 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Mon May 17 00:00:00 EDT 2021

Coagulation factor V is a marker of tumor-infiltrating immune cells in breast cancer.

Oncoimmunology

Tinholt M,Stavik B,Tekpli X,Garred Ø,Borgen E,Kristensen V,Sahlberg KK,Sandset PM,Iversen N

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Unique natural killer cell subpopulations are associated with exacerbation risk in chronic obstructive pulmonary disease.

Scientific reports

Osterburg AR,Lach L,Panos RJ,Borchers MT

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Mon Jan 27 00:00:00 EST 2020

Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.

eLife

Gonzalo-Gil E,Rapuano PB,Ikediobi U,Leibowitz R,Mehta S,Coskun AK,Porterfield JZ,Lampkin TD,Marconi VC,Rimland D,Walker BD,Deeks S,Sutton RE

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Tue Apr 09 00:00:00 EDT 2019

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+ and CD8+ T Cells.

Frontiers in pharmacology

Gerner MC,Niederstaetter L,Ziegler L,Bileck A,Slany A,Janker L,Schmidt RLJ,Gerner C,Del Favero G,Schmetterer KG

48-0699 was used in Flow cytometry/Cell sorting to identify new targets for the control of immune cell properties using proteomics.

Sun Jan 10 00:00:00 EST 2021

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

48-0699 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes.

Fri Mar 01 00:00:00 EST 2019

Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase.

Chinese medicine

Houng JY,Tai TS,Hsu SC,Hsu HF,Hwang TS,Lin CJ,Fang LW

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Scientific reports

Albert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

The tryptophan metabolite picolinic acid suppresses proliferation and metabolic activity of CD4+ T cells and inhibits c-Myc activation.

Journal of leukocyte biology

Prodinger J,Loacker LJ,Schmidt RL,Ratzinger F,Greiner G,Witzeneder N,Hoermann G,Jutz S,Pickl WF,Steinberger P,Marculescu R,Schmetterer KG

48-0699 was used in Flow cytometry/Cell sorting to investigate the effect of picolinic acid on cell viability, c-MYC activation and CD4+ T cell effector functions.

Fri Apr 01 00:00:00 EDT 2016

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells.

eLife

Reeder JE,Kwak YT,McNamara RP,Forst CV,D'Orso I

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Thu Apr 10 00:00:00 EDT 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

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Gene Aliases: AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3

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UniProt ID: (Human) Q07108

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Entrez Gene ID: (Human) 969

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Function(s)

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding

Process(es)

signal transduction cellular response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD69 Monoclonal Antibody (FN50), eFluor™ 450, eBioscience™

CD69 Antibody (48-0699-42) in Flow

Product Details

48-0699-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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