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Invitrogen
CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience™
FIGURE: 1 / 1
CD70 Antibody (16-0701-82) in Flow
Staining of 4-day LPS activated BALB/c splenocytes with 0.25 µg of Rat IgG2b kappa Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.25 µg of Anti-Mouse CD70 Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85)and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
16-0701-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FR70
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468997
Product Specific Information
Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis. FR70 has also been reported in blocking of CD70 in vitro .
Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD27 ligand; CD27-L; CD70; CD70 antigen; Ki-24 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Gene Aliases: Cd27l; CD27LG; Cd70; Tnfsf7
UniProt ID: (Mouse) O55237
Entrez Gene ID: (Mouse) 21948
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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