Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

26 Published Figures

28 References

View all (139) Transferrin Receptor (CD71) antibodies

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Datasheet

Protocols

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CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 29

CD71 (Transferrin Receptor) Antibody (12-0719-42) in Flow

Knockout of CD71 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR944258_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD71 was performed by staining HeLa CD71 knock out cells with 0.06 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-42, yellow histogram) and 0.2 mg/mL CD7... View More

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Product Details

12-0719-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT9 (OKT-9)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717077

Product Specific Information

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

A group B Streptococcus alpha-like protein subunit vaccine induces functionally active antibodies in humans targeting homotypic and heterotypic strains.

Cell reports. Medicine

Pawlowski A,Lannergård J,Gonzalez-Miro M,Cao D,Larsson S,Persson JJ,Kitson G,Darsley M,Rom AL,Hedegaard M,Fischer PB,Johansson-Lindbom B

12-0719-42 was used in Flow Cytometry to demonstrate robust IgG and IgA responses in relation to vaccine homotypic and heterotypic group B Streptococcus strains, which go on to prevent bacterial invasion of epithelial cells in neonates.

Tue Feb 15 00:00:00 EST 2022

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

Blocking transferrin receptor inhibits the growth of lung adenocarcinoma cells in vitro.

Thoracic cancer

Wu Y,Xu J,Chen J,Zou M,Rusidanmu A,Yang R

Thu Feb 01 00:00:00 EST 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication.

The EMBO journal

Amaral AJ,Andrade J,Foxall RB,Matoso P,Matos AM,Soares RS,Rocha C,Ramos CG,Tendeiro R,Serra-Caetano A,Guerra-Assunção JA,Santa-Marta M,Gonçalves J,Gama-Carvalho M,Sousa AE

12-0719 was used in Flow cytometry/Cell sorting to identify potential regulators of cell activation in T cell memory differentiation and HIV infection.

Wed Feb 01 00:00:00 EST 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

The tryptophan metabolite picolinic acid suppresses proliferation and metabolic activity of CD4+ T cells and inhibits c-Myc activation.

Journal of leukocyte biology

Prodinger J,Loacker LJ,Schmidt RL,Ratzinger F,Greiner G,Witzeneder N,Hoermann G,Jutz S,Pickl WF,Steinberger P,Marculescu R,Schmetterer KG

12-0719 was used in Flow cytometry/Cell sorting to investigate the effect of picolinic acid on cell viability, c-MYC activation and CD4+ T cell effector functions.

Fri Apr 01 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

Structure-function analysis of heterodimer formation, oligomerization, and receptor binding of the Staphylococcus aureus bi-component toxin LukGH.

The Journal of biological chemistry

Badarau A,Rouha H,Malafa S,Logan DT,Håkansson M,Stulik L,Dolezilkova I,Teubenbacher A,Gross K,Maierhofer B,Weber S,Jägerhofer M,Hoffman D,Nagy E

12-0719 was used in Flow cytometry/Cell sorting to detect weak binding of LukH, but not LukG, to the cellular receptor CD11b by biolayer interferometry.

Fri Jan 02 00:00:00 EST 2015

The cyclin dependent kinase inhibitor (R)-roscovitine mediates selective suppression of alloreactive human T cells but preserves pathogen-specific and leukemia-specific effectors.

Clinical immunology (Orlando, Fla.)

Nellore A,Liu B,Patsoukis N,Boussiotis VA,Li L

12-0719 was used in Flow cytometry/Cell sorting to examine the effects of pharmacologic Cdk2 inhibition on alloreactive human T cells.

Wed Jul 02 00:00:00 EDT 2014

Developmental transcriptome analysis of human erythropoiesis.

Human molecular genetics

Shi L,Lin YH,Sierant MC,Zhu F,Cui S,Guan Y,Sartor MA,Tanabe O,Lim KC,Engel JD

12-0719 was used in Flow cytometry/Cell sorting to perform RNA-seq on CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages.

Mon Sep 01 00:00:00 EDT 2014

Lysine-specific demethylase 1 is a therapeutic target for fetal hemoglobin induction.

Nature medicine

Shi L,Cui S,Engel JD,Tanabe O

12-0719 was used in Flow cytometry/Cell sorting to examine the effect of lysine-specific demethylase 1 inhibition on the expression of foetal γ-globin.

Fri Mar 01 00:00:00 EST 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 12-0719-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Dompe N,Rivers CS,Li L,Cordes S,Schwickart M,Punnoose EA,Amler L,Seshagiri S,Tang J,Modrusan Z,Davis DP

12-0719 was used in Flow cytometry/Cell sorting to highlight a 8q22 gene cluster-mediated mechanism by which cancer cells may evade apoptosis.

Tue Oct 25 00:00:00 EDT 2011

Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells.

PloS one

Sjeklocha LM,Park CW,Wong PY,Roney MJ,Belcher JD,Kaufman DS,Vercellotti GM,Hebbel RP,Steer CJ

12-0719 was used in Flow cytometry/Cell sorting to investigate methods for efficient delivery of tightly regulated and stably expressed gene products, showing erythroid-specific expression of β-globin using the Sleeping Beauty transposon system.

Mon May 21 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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Function(s)

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

Process(es)

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import

Performance Guarantee

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CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PE, eBioscience™

CD71 (Transferrin Receptor) Antibody (12-0719-42) in Flow

Product Details

12-0719-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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