Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

28 Published Figures

26 References

View all (139) Transferrin Receptor (CD71) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 36

CD71 (Transferrin Receptor) Antibody (14-0719-82) in ICC/IF

Immunofluorescence analysis of CD71 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD71(Transferrin Receptor) Mouse Monoclonal Antibody (OKT9 (OKT-9)) (Product # 14... View More

CD71 (Transferrin Receptor) Antibody in Western Blot (WB)

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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Product Details

14-0719-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 21 publications 21 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OKT9 (OKT-9)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467338

Product Specific Information

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported: The OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT9 (OKT-9) antibody has been tested by flow cytometric analysis of unstimulated and CD3/CD28-stimulated (3 days) human blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

14-0719-82 was used in Flow Cytometry to reveal associations between cellular profiles and disease severity, opening opportunities to study immunopathology in dengue disease and the potential predictive value of these parameters.

Tue May 18 00:00:00 EDT 2021

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

JAK2/IDH-mutant-driven myeloproliferative neoplasm is sensitive to combined targeted inhibition.

The Journal of clinical investigation

McKenney AS,Lau AN,Somasundara AVH,Spitzer B,Intlekofer AM,Ahn J,Shank K,Rapaport FT,Patel MA,Papalexi E,Shih AH,Chiu A,Freinkman E,Akbay EA,Steadman M,Nagaraja R,Yen K,Teruya-Feldstein J,Wong KK,Rampal R,Vander Heiden MG,Thompson CB,Levine RL

14-0719 was used in Flow cytometry/Cell sorting to suggest that combined JAK and IDH inhibition may offer a therapeutic advantage in this high-risk MPN subtype.

Thu Feb 01 00:00:00 EST 2018

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Noninvasive detection of tumor-infiltrating T cells by PET reporter imaging.

The Journal of clinical investigation

McCracken MN,Vatakis DN,Dixit D,McLaughlin J,Zack JA,Witte ON

14-0719 was used in Flow cytometry/Cell sorting to evaluate the use of hdCK3mut reporter imaging in immunotherapy models.

Fri May 01 00:00:00 EDT 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Immunofluorescence

Thu May 02 00:00:00 EDT 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Ferristatin II promotes degradation of transferrin receptor-1 in vitro and in vivo.

PloS one

Byrne SL,Buckett PD,Kim J,Luo F,Sanford J,Chen J,Enns C,Wessling-Resnick M

14-0719 was used in Immunohistochemistry-immunofluorescence to show that ferristatin II degrades transferrin receptor-1 through a nystatin-sensitive lipid raft pathway in rats.

Thu Mar 27 00:00:00 EDT 2014

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 14-0719-82) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells.

Glycobiology

Pérez-Oliva AB,Martínez-Esparza M,Vicente-Fernández JJ,Corral-San Miguel R,García-Peñarrubia P,Hernández-Caselles T

14-0719 was used in Immunofluorescence to determine the expression and localisation of the two CD33 isoforms on several hematopoietic cell lines.

Wed Jun 01 00:00:00 EDT 2011

Inhibition of tumor metastasis: functional immune modulation of the CUB domain containing protein 1.

Molecular pharmaceutics

Fukuchi K,Steiniger SC,Deryugina E,Liu Y,Lowery CA,Gloeckner C,Zhou B,Kaufmann GF,Quigley JP,Janda KD

14-0719 was used in Functional assays to indicate a functional role for CDCP1 in human cancers and highlight the therapeutic potential of function-blocking anti-CDCP1 antibodies.

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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Function(s)

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

Process(es)

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), eBioscience™

CD71 (Transferrin Receptor) Antibody (14-0719-82) in ICC/IF

Product Details

14-0719-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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