Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

30 Published Figures

25 References

View all (139) Transferrin Receptor (CD71) antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 33

CD71 (Transferrin Receptor) Antibody (25-0719-42) in Flow

Staining of 3-day Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0039-81 and Product # 16-0289-81)-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD71 (Transferrin Receptor) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Prod... View More

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Product Details

25-0719-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT9 (OKT-9)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573366

Product Specific Information

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

CDKN1A is a target for phagocytosis-mediated cellular immunotherapy in acute leukemia.

Nature communications

Allouch A,Voisin L,Zhang Y,Raza SQ,Lecluse Y,Calvo J,Selimoglu-Buet D,de Botton S,Louache F,Pflumio F,Solary E,Perfettini JL

25-0719-42 was used in Flow cytometry/Cell sorting to reveal p21 as a trigger of phagocytosis-guided pro-inflammatory TAM reprogramming and highlight the potential for p21TD-Mo-based cellular therapy as a cancer immunotherapy.

Tue Nov 08 00:00:00 EST 2022

Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells.

Cell chemical biology

Mehta S,Buyanbat A,Kai Y,Karayel O,Goldman SR,Seruggia D,Zhang K,Fujiwara Y,Donovan KA,Zhu Q,Yang H,Nabet B,Gray NS,Mann M,Fischer ES,Adelman K,Orkin SH

25-0719-42 was used in Flow Cytometry to leverage the dTAG PROTAC degradation platform to acutely deplete BCL11A protein in erythroid cells and examined consequences by nascent transcriptomics, proteomics, chromatin accessibility, and histone profiling.

Thu Aug 18 00:00:00 EDT 2022

ZNF410 represses fetal globin by singular control of CHD4.

Nature genetics

Vinjamur DS,Yao Q,Cole MA,McGuckin C,Ren C,Zeng J,Hossain M,Luk K,Wolfe SA,Pinello L,Bauer DE

25-0719-42 was used in Flow cytometry/Cell sorting to identify zinc-finger protein (ZNF) 410 as an fetal hemoglobin repressor.

Sat May 01 00:00:00 EDT 2021

Common variants in signaling transcription-factor-binding sites drive phenotypic variability in red blood cell traits.

Nature genetics

Choudhuri A,Trompouki E,Abraham BJ,Colli LM,Kock KH,Mallard W,Yang ML,Vinjamur DS,Ghamari A,Sporrij A,Hoi K,Hummel B,Boatman S,Chan V,Tseng S,Nandakumar SK,Yang S,Lichtig A,Superdock M,Grimes SN,Bowman TV,Zhou Y,Takahashi S,Joehanes R,Cantor AB,Bauer DE,Ganesh SK,Rinn J,Albert PS,Bulyk ML,Chanock SJ,Young RA,Zon LI

25-0719 was used in Flow cytometry/Cell sorting to propose that the majority of the RBC-trait-associated variants that reside on transcription-factor-binding sequences fall in STF target sequences, suggesting that the phenotypic variation of RBC traits could stem from altered responsiveness to extracellular stimuli.

Tue Dec 01 00:00:00 EST 2020

The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.

Development (Cambridge, England)

Creed TM,Baldeosingh R,Eberly CL,Schlee CS,Kim M,Cutler JA,Pandey A,Civin CI,Fossett NG,Kingsbury TJ

25-0719 was used in Flow cytometry/Cell sorting to investigate novel PAX-SIX-EYA-DACH roles in vivo in Drosophila hematopoiesis and in human erythropoiesis in vitro Using Drosophila genetics.

Fri Jan 03 00:00:00 EST 2020

The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.

Development (Cambridge, England)

Creed TM,Baldeosingh R,Eberly CL,Schlee CS,Kim M,Cutler JA,Pandey A,Civin CI,Fossett NG,Kingsbury TJ

25-0719 was used in Flow cytometry/Cell sorting to investigate novel PAX-SIX-EYA-DACH roles in vivo in Drosophila hematopoiesis and in human erythropoiesis in vitro Using Drosophila genetics.

Fri Jan 03 00:00:00 EST 2020

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

25-0719 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Chronic Exposure to Malaria Is Associated with Inhibitory and Activation Markers on Atypical Memory B Cells and Marginal Zone-Like B Cells.

Frontiers in immunology

Ubillos I,Campo JJ,Requena P,Ome-Kaius M,Hanieh S,Rose H,Samol P,Barrios D,Jiménez A,Bardají A,Mueller I,Menéndez C,Rogerson S,Moncunill G,Dobaño C

25-0719 was used in Flow cytometry/Cell sorting to test whether the expansion of atypical memory B cells and reduction of activated peripheral marginal zone-like B cells in constantly exposed individuals might be accompanied by phenotypic changes that would confer a tolerogenic profile, helping to establish tolerance to infections.

Wed Nov 20 00:00:00 EST 2019

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 25-0719-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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Function(s)

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

Process(es)

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PE-Cyanine7, eBioscience™

CD71 (Transferrin Receptor) Antibody (25-0719-42) in Flow

Product Details

25-0719-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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