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Invitrogen
CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Ultra Violet™ 737, eBioscience™
FIGURE: 1 / 1
CD80 (B7-1) Antibody (367-0801-82) in Flow
BALB/c mouse splenocytes were unstimulated (left) or stimulated for 72 hours with LPS (Product # 00-4976-03) (right). Cells were then stained with 0.06 µg of Armenian Hamster IgG Isotype Control, Brilliant Ultra Violet 737 (Product # 367-4888-81) (blue histogram) or 0.06 µg of CD80 (B7-1) Monoclonal Antibody, Brilliant Ultra Violet 737 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
367-0801-82
Product Specifications
Species Reactivity
Dog,
Mouse,
Pig
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
16-10A1
Conjugate
Excitation/Emission Max
350/740 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2942136
Product Specific Information
Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.
Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation B7-1 antigen; B7; B7 protein; B7-1 protein; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); sCD 80; sCD80; soluble CD 80; soluble CD80; T-cell co-stimulatory protein B7-1; T-cell costimulatory molecule; T-lymphocyte activation antigen CD80; TS/A-1
Gene Aliases: B7; B7-1; B71; Cd28l; Cd80; Ly-53; Ly53; MIC17; TSA1
UniProt ID: (Mouse) Q00609
Entrez Gene ID: (Pig) 397161, (Dog) 403765, (Mouse) 12519
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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