Invitrogen

CD81 Monoclonal Antibody (1.3.3.22)

Advanced Verification

IP-mass spectrometry

13 Published Figures

21 References

View all (66) CD81 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

CD81 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

CD81 Antibody (MA5-13548) in ICC/IF

Immunofluorescent analysis of CD81 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD81 monoclonal antibody (Product # MA5-13548) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjuga... View More

CD81 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

CD81 Antibody — IP-MS enrichment of CD81 (LFQ intensity): CD81 was enriched 1611-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CD81 antibody (Product # MA5-13548). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA5-13548

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 17 publications 17 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1.3.3.22

Immunogen

B cell line derived from a Burkitt lymphoma

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987151

Product Specific Information

MA5-13548 targets CD81 in WB, FACS, FN, ICC/IF, and IHC (F) applications and shows reactivity with Human and Rat samples.

The MA5-13548 immunogen is b cell line derived from a Burkitt lymphoma.

Target Information

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal miR-23b from bone marrow mesenchymal stem cells alleviates oxidative stress and pyroptosis after intracerebral hemorrhage.

Neural regeneration research

Hu LT,Wang BY,Fan YH,He ZY,Zheng WX

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Wed Mar 01 00:00:00 EST 2023

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

MA5-13548 was used in Western Blotting to identify potential exosomal miRNAs that respond to radiotherapy, plasma exosomal miRNAs from ESCC patients undergoing radiotherapy were isolated and sequenced.

Thu Sep 01 00:00:00 EDT 2022

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

Thu Sep 01 00:00:00 EDT 2022

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells.

Cells

Santos MF,Rappa G,Fontana S,Karbanová J,Aalam F,Tai D,Li Z,Pucci M,Alessandro R,Morimoto C,Corbeil D,Lorico A

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Wed Aug 10 00:00:00 EDT 2022

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

Laso-García F,Piniella D,Gómez-de Frutos MC,Casado-Fernández L,Pérez-Mato M,Alonso-López E,Otero-Ortega L,Bravo SB,Chantada-Vázquez MDP,Trilla-Fuertes L,Fresno-Vara JÁ,Fuentes B,Díez-Tejedor E,Gutiérrez-Fernández M,Alonso De Leciñana M

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Tue Feb 14 00:00:00 EST 2023

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Tue Feb 14 00:00:00 EST 2023

Functional Characterization of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells.

International journal of molecular sciences

Fan X,Cyganek L,Nitschke K,Uhlig S,Nuhn P,Bieback K,Duerschmied D,El-Battrawy I,Zhou X,Akin I

MA5-13548 was used in Western Blotting to demonstrate that hiPSC-ECs are similar to native endothelial cells in ion channel function and membrane receptor-coupled signaling and physiological cell functions, although some differences exist.

Sun Jul 31 00:00:00 EDT 2022

Functional Characterization of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells.

International journal of molecular sciences

Fan X,Cyganek L,Nitschke K,Uhlig S,Nuhn P,Bieback K,Duerschmied D,El-Battrawy I,Zhou X,Akin I

Sun Jul 31 00:00:00 EDT 2022

CD14 and CD26 from serum exosomes are associated with type 2 diabetes, exosomal Cystatin C and CD14 are associated with metabolic syndrome and atherogenic index of plasma.

PeerJ

Pérez-Macedonio CP,Flores-Alfaro E,Alarcón-Romero LDC,Vences-Velázquez A,Castro-Alarcón N,Martínez-Martínez E,Ramirez M

MA5-13548 was used in Western Blotting to evaluate the association of cystatin C, CD26, and CD14 proteins in serum exosomes from patients with Type 2 Diabetes (T2D), metabolic syndrome (MetS), and atherogenic index of plasma (AIP).

Tue Feb 14 00:00:00 EST 2023

Late Breaking Orals.

Journal of extracellular vesicles

No authors listed

MA5-13548 was used in Western Blotting to investagate a hypothesis that extracellular vesicles (EVs) released from Leishmania-infected cells (LiEVs) that contain parasite derived molecules, induce cellular responses that promote tissue remodeling.

Mon Aug 01 00:00:00 EDT 2022

Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells.

Small (Weinheim an der Bergstrasse, Germany)

Zarubova J,Hasani-Sadrabadi MM,Norris SCP,Majedi FS,Xiao C,Kasko AM,Li S

MA5-13548 was used in Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to develop an in vitro platform based on inverted-pyramidal microwells to follow and quantify the collective migration of hundreds of tumor cell clusters at once.

Thu Dec 01 00:00:00 EST 2022

Differential Immunomodulatory Effects of Head and Neck Cancer-Derived Exosomes on B Cells in the Presence of ATP.

International journal of molecular sciences

Coray M,Göldi V,Schmid L,Benecke L,Figueiró F,Muller L

MA5-13548 was used in Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to indicate that TEX play an important, but complex, role in the tumor microenvironment.

Mon Nov 21 00:00:00 EST 2022

Exosomes in serum‑free cultures of THP‑1 macrophages infected with Mycobacterium tuberculosis.

Molecular medicine reports

Biadglegne F,Rademacher P,De Sulbaran YGJ,König B,Rodloff AC,Zedler U,Dorhoi A,Sack U

MA5-13548 was used in Western Blotting to characterize exosomes released from THP‚1 macrophages cultured in serum‚free, ultra‚centrifuged medium upon infection with the human pathogen Mycobacterium tuberculosis (Mtb).

Mon Nov 01 00:00:00 EDT 2021

Exosomes in serum‑free cultures of THP‑1 macrophages infected with Mycobacterium tuberculosis.

Molecular medicine reports

Biadglegne F,Rademacher P,De Sulbaran YGJ,König B,Rodloff AC,Zedler U,Dorhoi A,Sack U

Mon Nov 01 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

MA5-13548 was used in Western Blotting to test if MSC-derived exosomes could accelerate wound healing by enhancing the biological properties of the main cell types involved in the key phases of this process.

Wed Jun 09 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Wed Jun 09 00:00:00 EDT 2021

Optimized method for extraction of exosomes from human primary muscle cells.

Skeletal muscle

Le Gall L,Ouandaogo ZG,Anakor E,Connolly O,Butler Browne G,Laine J,Duddy W,Duguez S

MA5-13548 was used in Western Blotting to optimise conditions for the in vitro cultivation of human myoblasts, and the quality and yield of exosomes extracted using an ultracentrifugation protocol are compared with a modified polymer-based precipitation strategy combined with extra washing steps.

Wed Jul 08 00:00:00 EDT 2020

Optimized method for extraction of exosomes from human primary muscle cells.

Skeletal muscle

Le Gall L,Ouandaogo ZG,Anakor E,Connolly O,Butler Browne G,Laine J,Duddy W,Duguez S

Wed Jul 08 00:00:00 EDT 2020

Electro-Acupuncture Promotes the Differentiation of Endogenous Neural Stem Cells via Exosomal microRNA 146b After Ischemic Stroke.

Frontiers in cellular neuroscience

Zhang S,Jin T,Wang L,Liu W,Zhang Y,Zheng Y,Lin Y,Yang M,He X,Lin H,Chen L,Tao J

MA5-13548 was used in Western Blotting to investigate the role of exosomal miRNAs involved electro-acupuncture and its regulation of endogenous neural stem cells for stroke recovery.

Mon Sep 28 00:00:00 EDT 2020

Electro-Acupuncture Promotes the Differentiation of Endogenous Neural Stem Cells via Exosomal microRNA 146b After Ischemic Stroke.

Frontiers in cellular neuroscience

Zhang S,Jin T,Wang L,Liu W,Zhang Y,Zheng Y,Lin Y,Yang M,He X,Lin H,Chen L,Tao J

MA5-13548 was used in Western Blotting to investigate the role of exosomal miRNAs involved electro-acupuncture and its regulation of endogenous neural stem cells for stroke recovery.

Mon Sep 28 00:00:00 EDT 2020

Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening.

Frontiers in genetics

Sorop A,Iacob R,Iacob S,Constantinescu D,Chitoiu L,Fertig TE,Dinischiotu A,Chivu-Economescu M,Bacalbasa N,Savu L,Gheorghe L,Dima S,Popescu I

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Sat Apr 16 00:00:00 EDT 2022

Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening.

Frontiers in genetics

Sorop A,Iacob R,Iacob S,Constantinescu D,Chitoiu L,Fertig TE,Dinischiotu A,Chivu-Economescu M,Bacalbasa N,Savu L,Gheorghe L,Dima S,Popescu I

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Sat Apr 16 00:00:00 EDT 2022

Integrated Dual-Mode Chromatography to Enrich Extracellular Vesicles from Plasma.

Advanced biosystems

Van Deun J,Jo A,Li H,Lin HY,Weissleder R,Im H,Lee H

MA5-13548 was used in Flow Cytometry to show a new separation approach, known as dual-mode chromatography (DMC), capable of enriching plasma EVs, and depleting LPPs is reported.

Tue Dec 01 00:00:00 EST 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Dinh PC,Paudel D,Brochu H,Popowski KD,Gracieux MC,Cores J,Huang K,Hensley MT,Harrell E,Vandergriff AC,George AK,Barrio RT,Hu S,Allen TA,Blackburn K,Caranasos TG,Peng X,Schnabel LV,Adler KB,Lobo LJ,Goshe MB,Cheng K

MA5-13548 was used in Western Blotting to show that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.

Fri Feb 28 00:00:00 EST 2020

Serum milk fat globule-EGF factor 8 (MFG-E8) as a diagnostic and prognostic biomarker in patients with hepatocellular carcinoma.

Scientific reports

Shimagaki T,Yoshio S,Kawai H,Sakamoto Y,Doi H,Matsuda M,Mori T,Osawa Y,Fukai M,Yoshida T,Ma Y,Akita T,Tanaka J,Taketomi A,Hanayama R,Yoshizumi T,Mori M,Kanto T

Published figure using CD81 monoclonal antibody (Product # MA5-13548) in Western Blot

Thu Oct 31 00:00:00 EDT 2019

JC Polyomavirus Uses Extracellular Vesicles To Infect Target Cells.

mBio

Morris-Love J,Gee GV,O'Hara BA,Assetta B,Atkinson AL,Dugan AS,Haley SA,Atwood WJ

MA5-13548 was used in Western Blotting to study the mechanism of human JC polyomavirus infection in vivo.

Tue Apr 09 00:00:00 EDT 2019

Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke.

Frontiers in molecular neuroscience

Kerr N,García-Contreras M,Abbassi S,Mejias NH,Desousa BR,Ricordi C,Dietrich WD,Keane RW,de Rivero Vaccari JP

MA5-13548 was used in Western Blotting to analyse serum and serum-derived extracellular vesicle samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain, IL-1Β, and IL-18.

Thu Oct 01 00:00:00 EDT 2020

Human embryonic stem cells extracellular vesicles and their effects on immortalized human retinal Müller cells.

PloS one

Peng Y,Baulier E,Ke Y,Young A,Ahmedli NB,Schwartz SD,Farber DB

MA5-13548 was used in Western Blotting to characterise extracellular vesicles derived from human embryonic stem cells and investigate the effects that they have on cultured retinal glial, progenitor Müller cells.

Fri Jun 22 00:00:00 EDT 2018

Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression.

Aging

Bhome R,Goh RW,Bullock MD,Pillar N,Thirdborough SM,Mellone M,Mirnezami R,Galea D,Veselkov K,Gu Q,Underwood TJ,Primrose JN,De Wever O,Shomron N,Sayan AE,Mirnezami AH

MA5-13548 was used in Western Blotting to identify and characterise exosomal microRNAs as mediators of stromal-tumour signalling.

Thu Dec 28 00:00:00 EST 2017

Bioinformatics

Protein Aliases: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28

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UniProt ID: (Human) P60033

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Entrez Gene ID: (Human) 975, (Rat) 25621

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Function(s)

protein binding MHC class II protein complex binding transferrin receptor binding

Process(es)

activation of MAPK activity phosphatidylinositol biosynthetic process protein localization positive regulation of cell proliferation response to wounding positive regulation of B cell proliferation regulation of growth regulation of cell proliferation positive regulation of 1-phosphatidylinositol 4-kinase activity phosphatidylinositol metabolic process positive regulation of peptidyl-tyrosine phosphorylation regulation of cell motility cell surface receptor signaling pathway cell proliferation receptor internalization regulation of protein stability positive regulation of transcription from RNA polymerase II promoter viral entry into host cell receptor-mediated virion attachment to host cell regulation of immune response protein localization to lysosome cellular response to low-density lipoprotein particle stimulus positive regulation of protein catabolic process in the vacuole

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CD81 Monoclonal Antibody (1.3.3.22)

CD81 Antibody (MA5-13548) in ICC/IF

Product Details

MA5-13548

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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